Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF85_RS13800 Genome accession   NZ_CP156776
Coordinates   2829586..2831295 (+) Length   569 a.a.
NCBI ID   WP_347453472.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7955     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2824586..2836295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF85_RS13755 (ABEF85_13755) nusA 2825173..2826657 (-) 1485 WP_347453476.1 transcription termination factor NusA -
  ABEF85_RS13760 (ABEF85_13760) rimP 2826693..2827217 (-) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF85_RS13790 (ABEF85_13790) secG 2828168..2828497 (-) 330 WP_347453474.1 preprotein translocase subunit SecG -
  ABEF85_RS13795 (ABEF85_13795) tpiA 2828511..2829302 (-) 792 WP_347453473.1 triose-phosphate isomerase -
  ABEF85_RS13800 (ABEF85_13800) pilB 2829586..2831295 (+) 1710 WP_347453472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF85_RS13805 (ABEF85_13805) pilC 2831327..2832556 (+) 1230 WP_347456717.1 type II secretion system F family protein Machinery gene
  ABEF85_RS13810 (ABEF85_13810) pilD 2832556..2833416 (+) 861 WP_347456716.1 A24 family peptidase Machinery gene
  ABEF85_RS13815 (ABEF85_13815) coaE 2833418..2834017 (+) 600 WP_347453469.1 dephospho-CoA kinase -
  ABEF85_RS13820 (ABEF85_13820) - 2834042..2834962 (-) 921 WP_347453468.1 DMT family transporter -
  ABEF85_RS13825 (ABEF85_13825) rlmB 2834999..2835748 (-) 750 WP_347453467.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF85_RS13830 (ABEF85_13830) - 2835901..2836224 (-) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63472.81 Da        Isoelectric Point: 4.8735

>NTDB_id=1002649 ABEF85_RS13800 WP_347453472.1 2829586..2831295(+) (pilB) [Acinetobacter thermotolerans strain ANC 7955]
MTAIQGSLRFTGFIRRLVDEGVISAPDMQSALVGAKKEKIDIVPYLIERHNISPTKIAETISTEFGEPLFDINAYDPALI
IREDFDEKIITKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDVDTSDPNKEEEETNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002649 ABEF85_RS13800 WP_347453472.1 2829586..2831295(+) (pilB) [Acinetobacter thermotolerans strain ANC 7955]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGAGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTGGTCGGCGCCAAAAAAGAAAAAATAGACATCGTTCCATATCTGATTGAACGCCATAATATTTCAC
CGACTAAAATCGCTGAAACCATTTCGACTGAATTTGGCGAACCGCTGTTTGACATCAATGCCTATGATCCTGCTCTGATC
ATTCGTGAAGACTTTGATGAAAAGATCATCACCAAACATCGCATCTTACCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCCACCAGCAACCCAACCAATGTTGAAGCCATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACCATCATTG
TCGAGCACAATAAACTCGAAAAGCTGATTGAACAGAATTTTACTGAAGAAAGTACCTTCGATTTTGATGATGACTTTGAT
CTAGATGTTGATGTTGATACCTCTGATCCGAATAAAGAGGAAGAAGAGACCAATACCGGTGATGAAGCACCGATTGTTAA
GTACATCAACAAGCTGCTAATTGATGCAATTCGTATGGGTGCATCAGACTTACACTTTGAACCGTATGAGAAAATATACC
GAGTCCGTTATCGTATTGACGGCGTATTGAGACAAGTGGCGACACCACCGCTGCAGCTTGCAACCCGTCTTTCCTCACGC
TTGAAAGTCATGTCGCAAATGGACATTTCAGAAAAACGGGTACCACAAGACGGTCGTATCAAGCTAAAACTTTCTAAAAA
TAAAGCAATTGATTTCCGTGTTAACTCGCTGCCGACCCTATTTGGTGAGAAAATTGTACTGCGTATCCTAGATCCATCCA
GTGCCATGCTTGGGATTGATGCATTGGGTTATGAACCTGATCAAAAAGCCCTGTTTATGGAAGCGCTGGACAAACCACAA
GGCATGCTGTTGATCACCGGTCCGACTGGTTCTGGTAAAACGGTTTCCTTATATACCGGTCTGAATATTCTAAATCGTGA
AGATACCAATATTTCTACCGCTGAAGACCCGGTGGAAATCAACTTACAAGGCATTAACCAGGTCAACGTTAACCCTAAAG
TCGGTTTAACCTTTGCTGCAGCCTTGAAATCTTTCCTGCGTCAGGATCCAGACATTGTCATGGTCGGGGAGATCCGAGAT
CTGGAAACTGCAGAAATCGCAGTGAAAGCTGCACAGACCGGTCACATGGTAATGTCAACACTACACACAAATAGTGCGCC
AGAAACACTAACCCGCTTACGCAATATGGGCGTACCGTCTTTTAACATTGCCACTTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCCCGCCGTTTATGTTCACAATGTAAAAAACCGGCCGATATTCCAAAGCAGAGCCTGCTAGAAATGGGCTTTACT
GAAGAAGATTTACAGAATCCGGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAAGGCCG
TGTCGGTATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.825

100

0.8
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.963

94.903

0.503
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio cholerae strain A1552

48.936

99.121

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilF Thermus thermophilus HB27

37.5

98.418

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

33.605

100

0.362

Multiple sequence alignment