Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFT82_RS21780 Genome accession   NZ_CP156749
Coordinates   4398002..4399705 (-) Length   567 a.a.
NCBI ID   WP_347505899.1    Uniprot ID   -
Organism   Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4393002..4404705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT82_RS21745 (ABFT82_21745) - 4393170..4393799 (-) 630 WP_160013159.1 DUF1780 domain-containing protein -
  ABFT82_RS21750 (ABFT82_21750) - 4393933..4394622 (+) 690 WP_090381696.1 energy-coupling factor ABC transporter permease -
  ABFT82_RS21755 (ABFT82_21755) - 4394727..4394942 (+) 216 WP_090381702.1 hypothetical protein -
  ABFT82_RS21760 (ABFT82_21760) yacG 4394946..4395146 (-) 201 WP_090381707.1 DNA gyrase inhibitor YacG -
  ABFT82_RS21765 (ABFT82_21765) coaE 4395143..4395757 (-) 615 WP_090381713.1 dephospho-CoA kinase -
  ABFT82_RS21770 (ABFT82_21770) pilD 4395908..4396777 (-) 870 WP_347505897.1 A24 family peptidase Machinery gene
  ABFT82_RS21775 (ABFT82_21775) pilC 4396781..4397998 (-) 1218 WP_347505898.1 type II secretion system F family protein Machinery gene
  ABFT82_RS21780 (ABFT82_21780) pilB 4398002..4399705 (-) 1704 WP_347505899.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFT82_RS21785 (ABFT82_21785) pilA2 4399927..4400361 (+) 435 WP_347505901.1 pilin Machinery gene
  ABFT82_RS21790 (ABFT82_21790) - 4400417..4402348 (+) 1932 WP_347505902.1 tetratricopeptide repeat protein -
  ABFT82_RS21795 (ABFT82_21795) - 4402345..4403193 (+) 849 WP_090381748.1 glycosyltransferase family 2 protein -
  ABFT82_RS21800 (ABFT82_21800) - 4403194..4404363 (+) 1170 WP_347505903.1 DegT/DnrJ/EryC1/StrS family aminotransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62574.40 Da        Isoelectric Point: 5.3352

>NTDB_id=1002534 ABFT82_RS21780 WP_347505899.1 4398002..4399705(-) (pilB) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
MNDSVSLTGLARQMVLAELLDEKTAQQAQSQAQRNKLSLVTYLVQNKLVKSRVLAELASEQFGVSFVDLNSIDKESQPKD
LVTEKLVRQHRVLPLWRRGNKLFIAVSDPTNHQAVTDIQFSTGMNTEAILVEDDKLGEAIEKFFDSTTSGIDDLGDIDLD
GLNVEAVSDDAQDNGDSNAADDAPVVRFVNKILLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEIARPPIQLSPRISAR
LKVMAGLDISERRKPQDGRIKMRISKSKSIDFRVSTCPTLWGEKIVMRILDSSSAQMGIDALGYEESQKELYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNNCKKEIQVPREALLEEGFP
ENKIDTFKIYEPTGCENCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDISIQMRKDGFNDLRTSALLKAMQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1002534 ABFT82_RS21780 WP_347505899.1 4398002..4399705(-) (pilB) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
ATGAACGATAGCGTGTCCCTTACCGGCCTTGCCCGCCAGATGGTTCTGGCAGAACTACTGGATGAAAAAACCGCTCAACA
GGCGCAGAGCCAGGCCCAGCGCAATAAACTGTCGCTGGTCACTTATCTGGTGCAGAACAAACTGGTTAAAAGTCGCGTGC
TTGCCGAGCTTGCCTCCGAGCAGTTTGGCGTATCCTTCGTCGATCTGAATTCGATAGACAAAGAAAGCCAACCCAAGGAT
CTAGTAACCGAAAAGCTGGTGCGCCAACACCGCGTGCTGCCGCTATGGCGTCGCGGCAACAAGCTATTTATCGCTGTTTC
CGACCCCACCAATCACCAAGCTGTCACCGACATCCAGTTCAGCACCGGAATGAACACCGAAGCCATCCTGGTGGAAGACG
ACAAGCTTGGTGAGGCTATCGAAAAATTCTTCGACTCGACCACAAGCGGAATAGACGACCTAGGGGATATAGATCTCGAC
GGCCTGAATGTTGAAGCGGTCAGTGACGATGCGCAGGACAACGGGGACAGCAATGCTGCCGATGACGCGCCAGTAGTACG
CTTTGTCAATAAGATATTGCTGGATGCCATTAAAGGCGGCTCATCTGACCTACACTTTGAGCCCTACGAGAAGGCATATC
GCGTACGCTTTCGCACAGACGGCATTCTGCATGAGATAGCTCGGCCGCCCATCCAGCTATCACCACGCATTTCTGCTCGA
TTGAAGGTTATGGCAGGCCTGGATATTTCCGAGCGCCGCAAGCCGCAGGATGGACGCATCAAAATGCGCATATCTAAAAG
CAAATCAATCGACTTTCGTGTAAGTACCTGCCCAACCCTGTGGGGCGAAAAGATCGTAATGCGGATTCTTGACTCCTCCA
GCGCGCAGATGGGTATCGACGCACTCGGTTATGAAGAGTCGCAAAAAGAGCTCTATATGTCCGCCCTGAAGCAGCCACAG
GGCATGATCCTGGTGACAGGCCCTACCGGCTCTGGCAAAACGGTCTCTCTCTATACAGGCCTGAATATCCTCAATACCGT
TGACGTAAACATCTCCACCGCTGAAGACCCCGTGGAAATCAATCTGGAAGGCATCAACCAAGTCAACGTAAACCCCAAAC
AGGGCATGGATTTTACTGCCGCACTGAAGTCATTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAGATTCGCGAC
CTGGATACCGCGTCCATCGCTATCAAAGCAGCGCAAACCGGCCACATGGTGATGTCCACGCTGCACACCAATAGCGCAGC
AGAAACCCTGACTCGCCTGCGCAATATGGGCGTGCCGTCGTTCAATATTGCTACGTCGGTCAACCTGATTATTGCTCAGC
GTCTGGCGCGCAAACTCTGTAATAACTGCAAAAAGGAAATACAGGTTCCCCGCGAGGCCCTACTCGAGGAAGGTTTCCCG
GAAAACAAAATCGACACCTTCAAGATTTATGAACCTACAGGCTGCGAAAACTGCAATGGCGGTTACAAAGGCCGTGTTGG
GATTTATGAAGTGGTTAAAAACACTCCAGCCCTGCAACGGATTATCATGGAGGAAGGCAACTCTATTGATATCTCCATCC
AGATGCGCAAAGACGGCTTTAATGACCTGCGTACCTCAGCGCTGCTGAAGGCCATGCAAGGCGTTACCAGCCTCGCTGAA
GTGAACCGCGTGACTAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.772

100

0.591
  pilB Acinetobacter baylyi ADP1

58.615

99.295

0.582
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.556
  pilB Vibrio cholerae strain A1552

50.714

98.765

0.501
  pilB Vibrio campbellii strain DS40M4

49.468

99.471

0.492
  pilF Neisseria gonorrhoeae MS11

48.936

99.471

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

51.02

95.062

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

93.651

0.369
  pilF Thermus thermophilus HB27

36.602

100

0.369

Multiple sequence alignment