Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AACH63_RS00780 Genome accession   NZ_AP027240
Coordinates   153875..154807 (+) Length   310 a.a.
NCBI ID   WP_286210749.1    Uniprot ID   -
Organism   Lactococcus formosensis strain L21-20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 136295..153372 153875..154807 flank 503


Gene organization within MGE regions


Location: 136295..154807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH63_RS00700 (LG21E20_01340) - 136295..137125 (+) 831 WP_096368812.1 glycosyltransferase -
  AACH63_RS00705 (LG21E20_01350) - 137224..137925 (+) 702 WP_096368811.1 glycosyltransferase family 2 protein -
  AACH63_RS00710 (LG21E20_01360) - 137933..138283 (+) 351 WP_017368733.1 DUF2304 domain-containing protein -
  AACH63_RS00715 (LG21E20_01370) - 138270..139214 (+) 945 WP_096368810.1 NAD-dependent epimerase/dehydratase family protein -
  AACH63_RS00720 (LG21E20_01380) - 139211..140644 (+) 1434 WP_096368809.1 lipopolysaccharide biosynthesis protein -
  AACH63_RS00725 (LG21E20_01390) - 140637..141776 (+) 1140 WP_185767487.1 glycosyltransferase family 4 protein -
  AACH63_RS00730 (LG21E20_01400) - 141773..142597 (+) 825 WP_096368807.1 phosphorylcholine transferase LicD -
  AACH63_RS00735 (LG21E20_01410) - 142618..143610 (+) 993 WP_096368806.1 glycosyltransferase -
  AACH63_RS00740 (LG21E20_01420) - 143612..144499 (+) 888 WP_270740735.1 glycosyltransferase family A protein -
  AACH63_RS00745 (LG21E20_01430) tagD 144524..144937 (+) 414 WP_096368804.1 glycerol-3-phosphate cytidylyltransferase -
  AACH63_RS00750 (LG21E20_01440) - 145022..146689 (+) 1668 WP_231951076.1 surface protein -
  AACH63_RS00755 (LG21E20_01450) - 146699..148066 (+) 1368 WP_096368802.1 DUF2142 domain-containing protein -
  AACH63_RS00760 (LG21E20_01460) - 148096..149700 (-) 1605 WP_096368801.1 GH25 family lysozyme -
  AACH63_RS00765 (LG21E20_01470) guaB 149874..151355 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  AACH63_RS00770 (LG21E20_01480) - 151711..153372 (+) 1662 WP_096369379.1 S-layer homology domain-containing protein -
  AACH63_RS00775 (LG21E20_01490) rpsU 153501..153677 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH63_RS00780 (LG21E20_01500) comGA 153875..154807 (+) 933 WP_286210749.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 310 a.a.        Molecular weight: 35338.39 Da        Isoelectric Point: 6.3944

>NTDB_id=100241 AACH63_RS00780 WP_286210749.1 153875..154807(+) (comGA) [Lactococcus formosensis strain L21-20]
MAKTLLQKAVDQMVHDLYLVALEGRYSLYFRTATERRFEEEVSLEQGQALIAHMKFLSGMNLGESRRVQLGACTYSLDKG
KQRLRLSTVGDFHGQESMVIRFLHHQQNQLYFWNSELFNTFMGGRGLYLFSGPVGSGKTSLMYKFAREYFKDQQVICIED
PVELVEAEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVVARLKEL
GLTDWELQSSLQRVIYQRLIAGKGLFVCEKEKFEEWQPDEWNGQIDQLVADGFISAATAAREKIEFSEAD

Nucleotide


Download         Length: 933 bp        

>NTDB_id=100241 AACH63_RS00780 WP_286210749.1 153875..154807(+) (comGA) [Lactococcus formosensis strain L21-20]
TTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTACTTAGTAGCATTAGAAGGGAGGTATTC
TCTATATTTTCGTACAGCAACAGAAAGGAGATTTGAAGAAGAAGTATCCCTTGAACAAGGGCAAGCTCTGATCGCTCATA
TGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGGGTACAGTTGGGGGCTTGCACTTATAGCTTAGATAAAGGT
AAGCAACGCTTACGTTTATCTACTGTCGGGGATTTTCATGGTCAAGAAAGTATGGTTATTCGATTCTTGCATCATCAACA
AAACCAACTTTATTTTTGGAACTCAGAGCTCTTTAACACTTTTATGGGTGGAAGAGGACTTTATCTGTTTTCAGGACCTG
TGGGGTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGAGAGTATTTTAAGGATCAACAAGTTATTTGTATAGAAGAT
CCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTTCAAGTCAATAAAGTTATAGGGAATGATTACGATGCCTTGATTAA
ACTTTCTCTTCGCCATAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCTAAGGCTGTGCTTCGTG
CAAGCTTGACTGGGTATACGGTGTTCTCCACTGTTCATGCACGCTCAATATCGGGTGTAGTTGCACGCCTAAAAGAACTT
GGATTAACTGATTGGGAACTACAATCCAGTCTTCAGCGAGTGATTTATCAGCGATTAATTGCAGGAAAGGGATTATTTGT
TTGTGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTGCAGATGGATTTA
TCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.871

99.677

0.597
  comYA Streptococcus gordonii str. Challis substr. CH1

52.885

100

0.532
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

51.78

99.677

0.516
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.78

99.677

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.456

99.677

0.513
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.456

99.677

0.513
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.456

99.677

0.513
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.456

99.677

0.513
  comYA Streptococcus mutans UA159

50.974

99.355

0.506
  comYA Streptococcus mutans UA140

50.974

99.355

0.506

Multiple sequence alignment