Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   AACH29_RS00730 Genome accession   NZ_AP027239
Coordinates   145723..146139 (+) Length   138 a.a.
NCBI ID   WP_014024177.1    Uniprot ID   -
Organism   Lactococcus garvieae strain L21-12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 140723..151139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH29_RS00705 (LG21E12_01350) guaB 141555..143036 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  AACH29_RS00710 (LG21E12_01360) rpsU 143159..143335 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH29_RS00715 (LG21E12_01370) comGA 143522..144466 (+) 945 WP_265150863.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH29_RS00720 (LG21E12_01380) comGB 144405..145424 (+) 1020 WP_016171027.1 competence type IV pilus assembly protein ComGB Machinery gene
  AACH29_RS00725 (LG21E12_01390) comYC 145443..145748 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  AACH29_RS00730 comGD 145723..146139 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  AACH29_RS00735 comGE 146171..146404 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  AACH29_RS00740 (LG21E12_01410) comYF 146385..146825 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  AACH29_RS00745 (LG21E12_01420) - 147058..147903 (+) 846 WP_023889974.1 zinc ABC transporter substrate-binding protein -
  AACH29_RS00750 (LG21E12_01430) - 147989..148858 (-) 870 WP_023889973.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 15820.51 Da        Isoelectric Point: 10.0791

>NTDB_id=100200 AACH29_RS00730 WP_014024177.1 145723..146139(+) (comGD) [Lactococcus garvieae strain L21-12]
MKNVMYRIKALTLLESLLVLFLCSFVLFLFSGSVKQSVRIARAEIFILQFERLYKDTQRMAGLKGQQQTLSASNGALYSQ
EEMLKVPEEAEINDFSITFDQKSGNSSLQKITIFLPYQKKTISYQLEIGSGKYKKRIT

Nucleotide


Download         Length: 417 bp        

>NTDB_id=100200 AACH29_RS00730 WP_014024177.1 145723..146139(+) (comGD) [Lactococcus garvieae strain L21-12]
ATGAAAAACGTAATGTACCGAATTAAAGCCTTGACTTTACTGGAGAGTTTACTCGTTCTTTTCCTATGCTCTTTTGTTCT
TTTTCTATTTTCAGGTTCGGTCAAACAAAGTGTGAGGATAGCACGCGCAGAGATTTTTATTTTACAATTTGAACGTCTTT
ATAAGGATACTCAACGCATGGCGGGACTAAAGGGACAGCAACAGACGTTGAGTGCGAGTAATGGTGCACTCTATAGCCAA
GAGGAAATGCTGAAAGTCCCAGAGGAGGCTGAAATAAATGATTTCTCTATTACTTTTGATCAAAAATCAGGCAATTCGAG
CCTACAAAAAATCACAATATTTCTTCCCTATCAGAAAAAAACAATCTCTTATCAATTGGAGATTGGAAGCGGAAAATATA
AGAAAAGAATCACTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Lactococcus lactis subsp. cremoris KW2

48.551

100

0.486
  comYD Streptococcus mutans UA140

41.667

95.652

0.399
  comYD Streptococcus mutans UA159

41.667

95.652

0.399
  comGD/cglD Streptococcus mitis SK321

40.31

93.478

0.377
  comGD/cglD Streptococcus pneumoniae TIGR4

39.535

93.478

0.37
  comGD/cglD Streptococcus mitis NCTC 12261

38.462

94.203

0.362
  comGD/cglD Streptococcus pneumoniae Rx1

38.76

93.478

0.362
  comGD/cglD Streptococcus pneumoniae D39

38.76

93.478

0.362
  comGD/cglD Streptococcus pneumoniae R6

38.76

93.478

0.362

Multiple sequence alignment