Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   AACH29_RS00740 Genome accession   NZ_AP027239
Coordinates   146385..146825 (+) Length   146 a.a.
NCBI ID   WP_014024179.1    Uniprot ID   -
Organism   Lactococcus garvieae strain L21-12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 141385..151825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH29_RS00705 (LG21E12_01350) guaB 141555..143036 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  AACH29_RS00710 (LG21E12_01360) rpsU 143159..143335 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH29_RS00715 (LG21E12_01370) comGA 143522..144466 (+) 945 WP_265150863.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH29_RS00720 (LG21E12_01380) comGB 144405..145424 (+) 1020 WP_016171027.1 competence type IV pilus assembly protein ComGB Machinery gene
  AACH29_RS00725 (LG21E12_01390) comYC 145443..145748 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  AACH29_RS00730 comGD 145723..146139 (+) 417 WP_014024177.1 competence type IV pilus minor pilin ComGD Machinery gene
  AACH29_RS00735 comGE 146171..146404 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  AACH29_RS00740 (LG21E12_01410) comYF 146385..146825 (+) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  AACH29_RS00745 (LG21E12_01420) - 147058..147903 (+) 846 WP_023889974.1 zinc ABC transporter substrate-binding protein -
  AACH29_RS00750 (LG21E12_01430) - 147989..148858 (-) 870 WP_023889973.1 RluA family pseudouridine synthase -
  AACH29_RS00755 (LG21E12_01440) - 148934..151222 (+) 2289 WP_014024183.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16835.45 Da        Isoelectric Point: 9.6906

>NTDB_id=100201 AACH29_RS00740 WP_014024179.1 146385..146825(+) (comYF) [Lactococcus garvieae strain L21-12]
MFLKKNKVTAFTLLESLLALLVLVGTFSLFLGMTKMFHEEVKRATTDHTQDWQLFCSLLRSELEGASLDKVENNYLYVRK
HVNLRFGLSSQGDFRKTNANGRGYQPMIHHLKNAKISQEGEQIKIILTFEKGGDRTFLYTFPEKES

Nucleotide


Download         Length: 441 bp        

>NTDB_id=100201 AACH29_RS00740 WP_014024179.1 146385..146825(+) (comYF) [Lactococcus garvieae strain L21-12]
TTGTTCTTGAAAAAAAATAAAGTTACAGCCTTTACACTTTTGGAATCACTCCTTGCTTTACTCGTGTTAGTGGGCACTTT
TTCTCTTTTTCTAGGTATGACTAAAATGTTTCATGAGGAAGTGAAACGTGCTACAACAGACCATACCCAAGACTGGCAAC
TTTTTTGTAGTCTACTGAGAAGTGAACTTGAGGGAGCAAGTTTAGATAAGGTAGAAAATAACTATCTTTATGTACGCAAA
CATGTTAACCTAAGGTTTGGCCTTTCCTCTCAAGGGGATTTTCGAAAAACTAACGCGAATGGACGAGGCTATCAGCCCAT
GATTCATCATTTAAAAAATGCAAAAATCAGCCAAGAGGGAGAGCAGATAAAAATTATCTTGACTTTTGAAAAAGGAGGAG
ACCGCACATTTCTTTACACGTTCCCAGAGAAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.355

96.575

0.486
  comYF Streptococcus mutans UA159

49.645

96.575

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

49.64

95.205

0.473
  comGF/cglF Streptococcus pneumoniae TIGR4

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae D39

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae Rx1

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae R6

40.972

98.63

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

38.889

98.63

0.384
  comGF/cglF Streptococcus mitis SK321

38.194

98.63

0.377

Multiple sequence alignment