Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU49_RS15010 Genome accession   NZ_CP156008
Coordinates   3427884..3429014 (-) Length   376 a.a.
NCBI ID   WP_012437803.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain NBC831     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3422884..3434014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU49_RS14990 (ABFU49_14960) - 3423971..3424510 (+) 540 WP_011037880.1 DNA-3-methyladenine glycosylase I -
  ABFU49_RS14995 (ABFU49_14965) - 3424507..3425781 (+) 1275 WP_274375585.1 hypothetical protein -
  ABFU49_RS15000 (ABFU49_14970) - 3426005..3426916 (+) 912 WP_274375584.1 DUF72 domain-containing protein -
  ABFU49_RS15005 (ABFU49_14975) - 3426930..3427604 (-) 675 WP_014508435.1 YitT family protein -
  ABFU49_RS15010 (ABFU49_14980) pilU 3427884..3429014 (-) 1131 WP_012437803.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU49_RS15015 (ABFU49_14985) pilT 3429151..3430188 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU49_RS15020 (ABFU49_14990) - 3430475..3431158 (+) 684 WP_349749514.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU49_RS15025 (ABFU49_14995) proC 3431199..3432059 (+) 861 WP_162808585.1 pyrroline-5-carboxylate reductase -
  ABFU49_RS15030 (ABFU49_15000) - 3432386..3432601 (-) 216 WP_165349445.1 hypothetical protein -
  ABFU49_RS15035 (ABFU49_15005) - 3432600..3433022 (+) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU49_RS15040 (ABFU49_15010) - 3433166..3433756 (+) 591 WP_011037887.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41982.19 Da        Isoelectric Point: 6.6612

>NTDB_id=1001117 ABFU49_RS15010 WP_012437803.1 3427884..3429014(-) (pilU) [Xanthomonas campestris pv. campestris strain NBC831]
MSTIDFTSFLKLMAHQNASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1001117 ABFU49_RS15010 WP_012437803.1 3427884..3429014(-) (pilU) [Xanthomonas campestris pv. campestris strain NBC831]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAATGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGAATGGTGCTGCGTCGGATCGAGACGCGCATTCC
CACCGTGGAGGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCCATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATTGATACCGATAG
CTGGGAAAATGCGTTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTACGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment