Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU32_RS07200 Genome accession   NZ_CP155982
Coordinates   1716182..1717312 (+) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas campestris pv. campestris strain CFBP 1121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1711182..1722312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU32_RS07175 (ABFU32_07185) - 1711449..1712039 (-) 591 WP_011037887.1 M23 family metallopeptidase -
  ABFU32_RS07180 (ABFU32_07190) - 1712183..1712605 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU32_RS07185 (ABFU32_07195) proC 1713150..1714010 (-) 861 WP_016944106.1 pyrroline-5-carboxylate reductase -
  ABFU32_RS07190 (ABFU32_07200) - 1714051..1714734 (-) 684 WP_011037884.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU32_RS07195 (ABFU32_07205) pilT 1715022..1716059 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU32_RS07200 (ABFU32_07210) pilU 1716182..1717312 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU32_RS07205 (ABFU32_07215) - 1717592..1718266 (+) 675 WP_014508435.1 YitT family protein -
  ABFU32_RS07210 (ABFU32_07220) - 1718280..1719179 (-) 900 WP_011037882.1 DUF72 domain-containing protein -
  ABFU32_RS07215 (ABFU32_07225) - 1719402..1720676 (-) 1275 WP_011037881.1 hypothetical protein -
  ABFU32_RS07220 (ABFU32_07230) - 1720673..1721212 (-) 540 WP_011037880.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1000691 ABFU32_RS07200 WP_005990431.1 1716182..1717312(+) (pilU) [Xanthomonas campestris pv. campestris strain CFBP 1121]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1000691 ABFU32_RS07200 WP_005990431.1 1716182..1717312(+) (pilU) [Xanthomonas campestris pv. campestris strain CFBP 1121]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGAATGGTGCTGCGTCGGATCGAGACGCGCATTCC
CACCGTGGAGGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCCATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATTGATACCGATAG
CTGGGAAAATGCGTTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTCGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment