Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU38_RS06985 Genome accession   NZ_CP155967
Coordinates   1661931..1663061 (+) Length   376 a.a.
NCBI ID   WP_039404792.1    Uniprot ID   A0AB34PCL6
Organism   Xanthomonas campestris pv. raphani strain BXC#141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656931..1668061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU38_RS06960 (ABFU38_06955) - 1657199..1657789 (-) 591 WP_080565893.1 M23 family metallopeptidase -
  ABFU38_RS06965 (ABFU38_06960) - 1657933..1658355 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU38_RS06970 (ABFU38_06965) proC 1658899..1659759 (-) 861 WP_274341798.1 pyrroline-5-carboxylate reductase -
  ABFU38_RS06975 (ABFU38_06970) - 1659800..1660483 (-) 684 WP_274341799.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU38_RS06980 (ABFU38_06975) pilT 1660771..1661808 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU38_RS06985 (ABFU38_06980) pilU 1661931..1663061 (+) 1131 WP_039404792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU38_RS06990 (ABFU38_06985) - 1663341..1664015 (+) 675 WP_228944705.1 YitT family protein -
  ABFU38_RS06995 (ABFU38_06990) - 1664029..1664928 (-) 900 WP_407298497.1 DUF72 domain-containing protein -
  ABFU38_RS07000 (ABFU38_06995) - 1665151..1666413 (-) 1263 WP_407469346.1 hypothetical protein -
  ABFU38_RS07005 (ABFU38_07000) - 1666410..1666949 (-) 540 WP_115577390.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41982.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1000430 ABFU38_RS06985 WP_039404792.1 1661931..1663061(+) (pilU) [Xanthomonas campestris pv. raphani strain BXC#141]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1000430 ABFU38_RS06985 WP_039404792.1 1661931..1663061(+) (pilU) [Xanthomonas campestris pv. raphani strain BXC#141]
ATGAGCACCATCGACTTCACATCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGCTGCGGCGGATCGAGACGCGTATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGTCAGGCGCCGGATGTGATCATGATCGGCGAAGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment