Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 474678..475212 Replicon chromosome
Accession NC_004668
Organism Enterococcus faecalis V583

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag EF_RS02510 Protein ID WP_002360769.1
Coordinates 474937..475212 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag EF_RS02505 Protein ID WP_002369771.1
Coordinates 474678..474944 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF_RS02470 (EF0505) 470728..471321 + 594 WP_002414802.1 replication-relaxation family protein -
EF_RS02475 (EF0506) 471327..471815 + 489 WP_010710133.1 hypothetical protein -
EF_RS02480 (EF0507) 472407..472673 + 267 WP_002377947.1 hypothetical protein -
EF_RS02485 (EF0508) 472706..473206 + 501 WP_002360775.1 DnaJ domain-containing protein -
EF_RS02490 (EF0509) 473219..473467 + 249 WP_002360773.1 DUF3850 domain-containing protein -
EF_RS02495 (EF0510) 473543..473959 + 417 WP_010710134.1 single-stranded DNA-binding protein -
EF_RS02500 (EF0511) 473983..474567 + 585 WP_002377949.1 thermonuclease family protein -
EF_RS02505 (EF0512) 474678..474944 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EF_RS02510 (EF0513) 474937..475212 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
EF_RS02515 (EF0514) 475403..476047 - 645 WP_002377952.1 IS6 family transposase -
EF_RS02520 476180..477062 + 883 Protein_477 IS256 family transposase -
EF_RS02525 (EF0516) 477367..478404 - 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
EF_RS02530 (EF0517) 478430..479368 - 939 WP_002370935.1 2-dehydropantoate 2-reductase -
EF_RS02535 (EF0518) 479496..479825 - 330 WP_002358661.1 LPXTG cell wall anchor domain-containing protein -
EF_RS02540 (EF0519) 479855..480040 + 186 WP_002358660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB 455113..505559 50446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T20900 WP_002360769.1 NC_004668:474937-475212 [Enterococcus faecalis V583]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T20900 NC_004668:474937-475212 [Enterococcus faecalis V583]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT20900 WP_002369771.1 NC_004668:474678-474944 [Enterococcus faecalis V583]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT20900 NC_004668:474678-474944 [Enterococcus faecalis V583]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References