Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 474675..475209 Replicon chromosome
Accession NZ_CP039296
Organism Enterococcus faecalis strain VE14089

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag E7T02_RS02680 Protein ID WP_002360769.1
Coordinates 474934..475209 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag E7T02_RS02675 Protein ID WP_002369771.1
Coordinates 474675..474941 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7T02_RS02640 470533..471318 + 786 WP_002360778.1 replication-relaxation family protein -
E7T02_RS02645 471324..471812 + 489 WP_010710133.1 hypothetical protein -
E7T02_RS02650 472404..472670 + 267 WP_002377947.1 hypothetical protein -
E7T02_RS02655 472703..473203 + 501 WP_002360775.1 DnaJ domain-containing protein -
E7T02_RS02660 473216..473464 + 249 WP_002360773.1 DUF3850 domain-containing protein -
E7T02_RS02665 473540..473956 + 417 WP_010710134.1 single-stranded DNA-binding protein -
E7T02_RS02670 473980..474564 + 585 WP_002377949.1 thermonuclease family protein -
E7T02_RS02675 474675..474941 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
E7T02_RS02680 474934..475209 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
E7T02_RS02685 475400..476080 - 681 Protein_473 IS6-like element IS1216 family transposase -
E7T02_RS02690 476165..477059 + 895 Protein_474 IS256 family transposase -
E7T02_RS02695 477364..478401 - 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
E7T02_RS02700 478427..479365 - 939 WP_002370935.1 2-dehydropantoate 2-reductase -
E7T02_RS02705 479493..479822 - 330 WP_002358661.1 LPXTG cell wall anchor domain-containing protein -
E7T02_RS02710 479852..480037 + 186 WP_002358660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB 455110..505556 50446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T123751 WP_002360769.1 NZ_CP039296:474934-475209 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T123751 NZ_CP039296:474934-475209 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT123751 WP_002369771.1 NZ_CP039296:474675-474941 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT123751 NZ_CP039296:474675-474941 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References