Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 2579472..2580006 Replicon chromosome
Accession NZ_CP040898
Organism Enterococcus faecalis strain HA-1

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag FHO10_RS13480 Protein ID WP_002360769.1
Coordinates 2579472..2579747 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag FHO10_RS13485 Protein ID WP_002369771.1
Coordinates 2579740..2580006 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHO10_RS13450 2574644..2574829 - 186 WP_002358660.1 hypothetical protein -
FHO10_RS13455 2574859..2575188 + 330 WP_002358661.1 LPXTG cell wall anchor domain-containing protein -
FHO10_RS13460 2575316..2576254 + 939 WP_002370935.1 2-dehydropantoate 2-reductase -
FHO10_RS13465 2576280..2577317 + 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
FHO10_RS13470 2577622..2578516 - 895 Protein_2399 IS256 family transposase -
FHO10_RS13475 2578601..2579281 + 681 Protein_2400 IS6-like element IS1216 family transposase -
FHO10_RS13480 2579472..2579747 - 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
FHO10_RS13485 2579740..2580006 - 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
FHO10_RS13490 2580117..2580701 - 585 WP_002377949.1 thermonuclease family protein -
FHO10_RS13495 2580725..2581141 - 417 WP_010710134.1 single-stranded DNA-binding protein -
FHO10_RS13500 2581217..2581465 - 249 WP_002360773.1 DUF3850 domain-containing protein -
FHO10_RS13505 2581478..2581978 - 501 WP_002360775.1 DnaJ domain-containing protein -
FHO10_RS13510 2582011..2582277 - 267 WP_002377947.1 hypothetical protein -
FHO10_RS13515 2582869..2583357 - 489 WP_010710133.1 hypothetical protein -
FHO10_RS13520 2583363..2584148 - 786 WP_002360778.1 replication-relaxation family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - esp / cylI / cylA / cylB / cylM / cylS / cylL / cylR1 / cylR2 / bsh 2535654..2599615 63961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T127201 WP_002360769.1 NZ_CP040898:c2579747-2579472 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T127201 NZ_CP040898:c2579747-2579472 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT127201 WP_002369771.1 NZ_CP040898:c2580006-2579740 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT127201 NZ_CP040898:c2580006-2579740 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References