Detailed information of regulator


Summary

Regulator ID REG0370 experimental
Regulator type protein
Regulatory pathway (from literatures) Environmental signal
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Glycine, serine and threonine metabolism (map00260); Cysteine and methionine metabolism (map00270); Lysine degradation (map00310); D-Amino acid metabolism (map00470)
Related T6SS T6SS01046 experimental (Type i4b)
Strain Acinetobacter baumannii strain ATCC 17978
Replicon chromosome
Sequence Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Description A ΔracK derivative of the Δ123 strain (Δ123ΔracK) lost most of its killing capacity, indicating that RacK enhances Tse4 toxicity. D-Lys secretion enhances the T6SS-mediated killing of A. baumannii.
Reference
External database links

Locus tag (Gene) ACX60_RS11355 (racK)
Coordinates (Strand) 2382673..2383965 (+)
NCBI ID WP_000769882.1
RefSeq NZ_CP012004
Uniprot ID A0A1E3M5A9_ACIBA
KEGG ID -
KEGG pathway (determined by BlastKOALA) map01100; map00260; map00270; map00310; map00470
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
ACX60_RS00605 (Tse4) ↓ WP_049594793.1
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Ala_racemase_N PF01168.22 7.1e-45 51..273
Ala_racemase_C PF00842.23 4.8e-35 283..408
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-430 MKLKTNFSHLAYSCALVLFGSMQFAHAAPLLTSHITNEVSPAPTANAWIEVDTQAFEDNIKRTNQLLNGKTQLCVVMKANAYGHSIDLLMPSVMKLNVSCIGITSNAEAAMVRKHGYKGKITRLRTATDSEIINAHALNIEELLGNYDQATRISKWAEANNTTVHYSLALNSGGMDRNGLEMSTAQGKEQAVAITKLPNLKIDGIMTHYAVEDEKYVRERLAQFNEQTAWLIKKAKLNRKELTLHTANSFATVNVPESHLDMVRAGAIIYGDFPNHPEYKRMMAFKTQIAAVNNYLKGSTVGYDQTYTLTRDSKLANLPVGYSDGYRRSFSNKAFVLVNGQKAPVVGRVSMNTVMVDVTDIPEAKAGDEVVLFGRQGNAEVQSSDLTSLTGHILTEAYTPWSNSNPQVIKPIQSPQQVILTNSKVAATAE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9955 pos: 27-28. AHA-AP. MKLKTNFSHLAYSCALVLFGSMQFAHA


  Protein sequence of regulator: 431 a.a.    .

>REG0370 NZ_CP012004:2382673-2383965 [Acinetobacter baumannii]
MKLKTNFSHLAYSCALVLFGSMQFAHAAPLLTSHITNEVSPAPTANAWIEVDTQAFEDNIKRTNQLLNGKTQLCVVMKAN
AYGHSIDLLMPSVMKLNVSCIGITSNAEAAMVRKHGYKGKITRLRTATDSEIINAHALNIEELLGNYDQATRISKWAEAN
NTTVHYSLALNSGGMDRNGLEMSTAQGKEQAVAITKLPNLKIDGIMTHYAVEDEKYVRERLAQFNEQTAWLIKKAKLNRK
ELTLHTANSFATVNVPESHLDMVRAGAIIYGDFPNHPEYKRMMAFKTQIAAVNNYLKGSTVGYDQTYTLTRDSKLANLPV
GYSDGYRRSFSNKAFVLVNGQKAPVVGRVSMNTVMVDVTDIPEAKAGDEVVLFGRQGNAEVQSSDLTSLTGHILTEAYTP
WSNSNPQVIKPIQSPQQVILTNSKVAATAE*

  Nucleotide sequence of regulator: 1293 bp    .

>REG0370 NZ_CP012004:2382673-2383965 [Acinetobacter baumannii]
ATGAAACTTAAAACAAATTTTTCGCATCTCGCTTATAGTTGTGCATTGGTATTATTCGGTTCCATGCAATTTGCCCATGC
TGCACCTTTACTCACGTCTCATATCACAAATGAAGTCAGCCCTGCACCTACAGCAAATGCATGGATTGAAGTTGATACTC
AAGCTTTTGAAGATAATATTAAAAGGACAAATCAATTACTTAATGGCAAAACACAACTTTGTGTAGTCATGAAAGCAAAT
GCATATGGGCATAGTATTGACCTACTTATGCCGAGTGTTATGAAGTTAAACGTATCGTGTATTGGCATCACCAGTAATGC
AGAAGCGGCGATGGTGCGTAAGCATGGCTATAAAGGTAAAATTACACGTTTACGTACAGCCACAGATAGTGAAATCATCA
ATGCACATGCGTTAAATATCGAAGAGCTTTTGGGTAATTATGATCAGGCTACCCGTATCTCTAAATGGGCAGAAGCGAAC
AATACAACTGTTCATTATAGCTTGGCTTTAAATTCGGGTGGTATGGATCGTAATGGTCTTGAAATGTCTACAGCTCAAGG
TAAAGAACAAGCTGTTGCAATCACTAAGCTTCCAAATCTTAAAATTGATGGCATTATGACCCATTATGCTGTAGAAGATG
AAAAATATGTTCGTGAGCGCCTAGCTCAATTTAATGAACAAACAGCATGGTTGATTAAAAAAGCCAAACTAAATCGTAAA
GAATTAACATTACATACAGCAAATTCATTTGCTACCGTAAATGTTCCTGAATCACATTTAGATATGGTCCGTGCAGGCGC
AATTATTTATGGAGATTTTCCTAATCACCCAGAATATAAACGCATGATGGCATTTAAAACACAAATTGCTGCAGTTAATA
ATTACCTTAAAGGTTCAACTGTAGGTTATGACCAAACTTATACGTTGACACGTGACTCCAAACTTGCAAACCTGCCTGTC
GGTTATTCGGATGGCTACCGTCGTAGTTTTAGTAATAAAGCTTTTGTCTTAGTCAATGGACAAAAAGCGCCAGTGGTTGG
TCGAGTATCAATGAATACTGTTATGGTTGATGTAACAGATATTCCAGAAGCCAAAGCGGGTGATGAAGTTGTATTATTTG
GACGTCAAGGCAATGCTGAGGTTCAATCTAGTGATTTAACGAGTCTTACAGGCCATATTTTGACAGAGGCCTATACTCCA
TGGAGTAACTCTAATCCACAAGTGATTAAACCTATTCAATCTCCACAACAAGTGATATTAACCAATAGTAAAGTTGCAGC
AACCGCTGAGTAA