Detailed information of regulator
Summary
| Regulator ID | REG0361  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01047  (Type i4b) |
| Strain | Acinetobacter baumannii strain Ab04-mff |
| Replicon | plasmid |
| Sequence | Protein sequence (191 a.a.); Nucleotide sequence (573 bp) |
| Description | Introduction of TetR1 or TetR2 dramatically decreased expression and secretion of Hcp in all T6+ Acinetobacter baumannii strains |
| Reference |
External database links
| Locus tag (Gene) | ACX61_19615 (tetR1) |
| Coordinates (Strand) | 139526..140098 (+) |
| NCBI ID | 487938137 |
| RefSeq | NZ_CP012007 |
| Uniprot ID | A0A1Y1P915_ACIBA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| hcp secretion | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TetR_N | PF00440.25 | 9.3e-10 | 16..62 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-190 | MEIEIMTKVISKRKTILDTALSLFKQYSFKFVGVDRIINESQVAKMTFYKHFPSKTLLIQACLCEEQKTIEESILSELSLLSEAGNIDRLKALLNWHVTYINQQNFNGCLFQKAVYENEVSEEVLSVIQAHKQWKFKLVSDLMEVPECSTAFVSSSMVYSMLEGMLLPANINPCVDHETAIKSLIQTFEA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9966 | - | - |
Protein sequence of regulator: 191 a.a. .
>REG0361 NZ_CP012007:139526-140098 [Acinetobacter baumannii]
MEIEIMTKVISKRKTILDTALSLFKQYSFKFVGVDRIINESQVAKMTFYKHFPSKTLLIQACLCEEQKTIEESILSELSL
LSEAGNIDRLKALLNWHVTYINQQNFNGCLFQKAVYENEVSEEVLSVIQAHKQWKFKLVSDLMEVPECSTAFVSSSMVYS
MLEGMLLPANINPCVDHETAIKSLIQTFEA*
MEIEIMTKVISKRKTILDTALSLFKQYSFKFVGVDRIINESQVAKMTFYKHFPSKTLLIQACLCEEQKTIEESILSELSL
LSEAGNIDRLKALLNWHVTYINQQNFNGCLFQKAVYENEVSEEVLSVIQAHKQWKFKLVSDLMEVPECSTAFVSSSMVYS
MLEGMLLPANINPCVDHETAIKSLIQTFEA*
Nucleotide sequence of regulator: 573 bp .
>REG0361 NZ_CP012007:139526-140098 [Acinetobacter baumannii]
ATGGAGATTGAAATCATGACTAAAGTTATTTCAAAAAGAAAAACCATTTTGGATACCGCTTTATCACTCTTTAAGCAGTA
CAGCTTTAAGTTTGTCGGTGTAGATCGGATCATCAATGAGTCACAAGTTGCGAAAATGACTTTTTATAAACACTTTCCGT
CTAAGACTTTGTTGATTCAGGCTTGTTTATGTGAAGAGCAAAAGACAATTGAAGAGTCTATTTTAAGTGAGCTGTCTTTA
CTTTCTGAGGCTGGAAATATCGACCGTTTAAAAGCATTGCTAAATTGGCATGTCACGTATATCAATCAGCAAAATTTTAA
TGGCTGTCTTTTTCAAAAAGCAGTGTATGAAAATGAGGTGAGTGAGGAAGTTTTATCCGTCATTCAAGCCCATAAACAAT
GGAAGTTCAAATTAGTTTCAGACTTAATGGAAGTTCCAGAATGCAGTACTGCCTTTGTCTCATCTTCGATGGTGTATAGC
ATGTTAGAAGGCATGCTCTTGCCTGCAAATATAAATCCATGTGTGGACCATGAAACAGCAATTAAGAGTCTAATTCAGAC
ATTTGAGGCTTAA
ATGGAGATTGAAATCATGACTAAAGTTATTTCAAAAAGAAAAACCATTTTGGATACCGCTTTATCACTCTTTAAGCAGTA
CAGCTTTAAGTTTGTCGGTGTAGATCGGATCATCAATGAGTCACAAGTTGCGAAAATGACTTTTTATAAACACTTTCCGT
CTAAGACTTTGTTGATTCAGGCTTGTTTATGTGAAGAGCAAAAGACAATTGAAGAGTCTATTTTAAGTGAGCTGTCTTTA
CTTTCTGAGGCTGGAAATATCGACCGTTTAAAAGCATTGCTAAATTGGCATGTCACGTATATCAATCAGCAAAATTTTAA
TGGCTGTCTTTTTCAAAAAGCAGTGTATGAAAATGAGGTGAGTGAGGAAGTTTTATCCGTCATTCAAGCCCATAAACAAT
GGAAGTTCAAATTAGTTTCAGACTTAATGGAAGTTCCAGAATGCAGTACTGCCTTTGTCTCATCTTCGATGGTGTATAGC
ATGTTAGAAGGCATGCTCTTGCCTGCAAATATAAATCCATGTGTGGACCATGAAACAGCAATTAAGAGTCTAATTCAGAC
ATTTGAGGCTTAA