Detailed information of regulator
Summary
| Regulator ID | REG0360  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01047  (Type i4b) |
| Strain | Acinetobacter baumannii strain Ab04-mff |
| Replicon | plasmid |
| Sequence | Protein sequence (190 a.a.); Nucleotide sequence (570 bp) |
| Description | Introduction of TetR1 or TetR2 dramatically decreased expression and secretion of Hcp in all T6+ Acinetobacter baumannii strains |
| Reference |
External database links
| Locus tag (Gene) | ACX61_19655 (tetR2) |
| Coordinates (Strand) | 144108..144677 (+) |
| NCBI ID | 446104352 |
| RefSeq | NZ_CP012007 |
| Uniprot ID | A0A009J8A1_ACIBA, A0A090BGE8_ACIBA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| hcp secretion | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TetR_N | PF00440.25 | 4.4e-08 | 21..66 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-189 | MTNKASQPRTFTLSDEKRIHILTTALNLFDQFGFQHISIMRIITEAKIAKQSFYMAFPSKDSFIIECLDMEISRLKQSLSNLLGQCGKDDHTSILKSFYQWHVDLAYREGYNGTLLTKAVIEYWNLPDIKMKVEDFNKWKYEIIAEYLAEEINNARLRIIVSAMNGILLPASTYLPTWEDIESLYGEQF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9976 | - | - |
Protein sequence of regulator: 190 a.a. .
>REG0360 NZ_CP012007:144108-144677 [Acinetobacter baumannii]
MTNKASQPRTFTLSDEKRIHILTTALNLFDQFGFQHISIMRIITEAKIAKQSFYMAFPSKDSFIIECLDMEISRLKQSLS
NLLGQCGKDDHTSILKSFYQWHVDLAYREGYNGTLLTKAVIEYWNLPDIKMKVEDFNKWKYEIIAEYLAEEINNARLRII
VSAMNGILLPASTYLPTWEDIESLYGEQF*
MTNKASQPRTFTLSDEKRIHILTTALNLFDQFGFQHISIMRIITEAKIAKQSFYMAFPSKDSFIIECLDMEISRLKQSLS
NLLGQCGKDDHTSILKSFYQWHVDLAYREGYNGTLLTKAVIEYWNLPDIKMKVEDFNKWKYEIIAEYLAEEINNARLRII
VSAMNGILLPASTYLPTWEDIESLYGEQF*
Nucleotide sequence of regulator: 570 bp .
>REG0360 NZ_CP012007:144108-144677 [Acinetobacter baumannii]
ATGACAAATAAAGCTTCACAGCCTAGAACATTTACATTATCTGATGAGAAGAGGATCCATATCTTAACGACAGCTCTAAA
CTTATTTGATCAGTTTGGTTTTCAGCATATAAGCATTATGCGTATTATCACTGAAGCAAAAATTGCTAAACAAAGTTTCT
ACATGGCTTTCCCTTCCAAAGATTCCTTCATCATTGAATGTTTAGATATGGAAATCTCTAGACTGAAGCAATCACTAAGC
AACCTCTTGGGCCAGTGTGGTAAAGATGACCATACAAGTATTTTAAAATCCTTCTATCAGTGGCACGTGGACCTAGCTTA
TAGAGAAGGATATAACGGTACGCTGTTAACAAAAGCTGTGATCGAATATTGGAATTTACCAGATATAAAAATGAAGGTTG
AAGACTTCAACAAATGGAAATATGAAATTATTGCAGAGTATCTGGCGGAAGAAATCAACAATGCTCGACTAAGAATTATA
GTAAGCGCAATGAATGGGATTTTATTGCCGGCTAGTACTTACTTGCCTACATGGGAAGATATTGAAAGTTTATATGGTGA
GCAATTTTGA
ATGACAAATAAAGCTTCACAGCCTAGAACATTTACATTATCTGATGAGAAGAGGATCCATATCTTAACGACAGCTCTAAA
CTTATTTGATCAGTTTGGTTTTCAGCATATAAGCATTATGCGTATTATCACTGAAGCAAAAATTGCTAAACAAAGTTTCT
ACATGGCTTTCCCTTCCAAAGATTCCTTCATCATTGAATGTTTAGATATGGAAATCTCTAGACTGAAGCAATCACTAAGC
AACCTCTTGGGCCAGTGTGGTAAAGATGACCATACAAGTATTTTAAAATCCTTCTATCAGTGGCACGTGGACCTAGCTTA
TAGAGAAGGATATAACGGTACGCTGTTAACAAAAGCTGTGATCGAATATTGGAATTTACCAGATATAAAAATGAAGGTTG
AAGACTTCAACAAATGGAAATATGAAATTATTGCAGAGTATCTGGCGGAAGAAATCAACAATGCTCGACTAAGAATTATA
GTAAGCGCAATGAATGGGATTTTATTGCCGGCTAGTACTTACTTGCCTACATGGGAAGATATTGAAAGTTTATATGGTGA
GCAATTTTGA