Detailed information of regulator
Summary
| Regulator ID | REG0335  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | IAA regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Tryptophan metabolism (map00380) |
| Related T6SS | T6SS01041  (Type i4b) |
| Strain | Pseudomonas savastanoi pv. savastanoi NCPPB 3335 |
| Replicon | chromosome |
| Sequence | Protein sequence (447 a.a.); Nucleotide sequence (1341 bp) |
| Description | β-Galactosidase activity shows the transcript levels of vgrG doubled in the presence of IAA, indicating a positive effect of IAA on the expression of the T6SS. |
| Reference |
External database links
| Locus tag (Gene) | PSA3335_RS05385 (iaaH) |
| Coordinates (Strand) | 1089578..1090918 (-) |
| NCBI ID | 488619198 |
| RefSeq | NZ_CP008742 |
| Uniprot ID | A0A2S6IZB9_PSESX, A0A0Q0B7D4_PSESS, A0A0P9W3P1_PSESS, A0A0N8QZM8_PSECA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map01100; map00380 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| The transcript levels of vgrG | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Amidase | PF01425.23 | 2.4e-81 | 45..434 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-446 | MHEIITLESLCQALADGEIAAAELRERALDTEARLARLNCFIREGDAVSQFGEADHAMKGTPLWGMPVSFKDNICVRGLPLTAGTRGMSGFVSDQDAAIVSQLRALGAVVAGKNNMHELSFGVTSINPHWGTVGNPVAPGYCAGGSSGGSAAAVASGIVPLSVGTDTGGSIRIPAAFCGITGFRPTTGRWSTAGIIPVSHTKDCVGLLTRTAGDAGFLYGLLSGKQQSFPLSRTAPCRIGLPVSMWSDLDGEVERACVNALSLLRKTGFEFIEIDDADIVELNQTLTFTVPLYEFFADLAQSLLSLGWKHGIHHIFAQVDDANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHNIELLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLASNAMLPSITIPVGPEGRLPVGLSFDALRGRDALLLSRVSAIEQVLER |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9607 | - | - |
Protein sequence of regulator: 447 a.a. .
>REG0335 NZ_CP008742:c1090918-1089578 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MHEIITLESLCQALADGEIAAAELRERALDTEARLARLNCFIREGDAVSQFGEADHAMKGTPLWGMPVSFKDNICVRGLP
LTAGTRGMSGFVSDQDAAIVSQLRALGAVVAGKNNMHELSFGVTSINPHWGTVGNPVAPGYCAGGSSGGSAAAVASGIVP
LSVGTDTGGSIRIPAAFCGITGFRPTTGRWSTAGIIPVSHTKDCVGLLTRTAGDAGFLYGLLSGKQQSFPLSRTAPCRIG
LPVSMWSDLDGEVERACVNALSLLRKTGFEFIEIDDADIVELNQTLTFTVPLYEFFADLAQSLLSLGWKHGIHHIFAQVD
DANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHNIELLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLAS
NAMLPSITIPVGPEGRLPVGLSFDALRGRDALLLSRVSAIEQVLER*
MHEIITLESLCQALADGEIAAAELRERALDTEARLARLNCFIREGDAVSQFGEADHAMKGTPLWGMPVSFKDNICVRGLP
LTAGTRGMSGFVSDQDAAIVSQLRALGAVVAGKNNMHELSFGVTSINPHWGTVGNPVAPGYCAGGSSGGSAAAVASGIVP
LSVGTDTGGSIRIPAAFCGITGFRPTTGRWSTAGIIPVSHTKDCVGLLTRTAGDAGFLYGLLSGKQQSFPLSRTAPCRIG
LPVSMWSDLDGEVERACVNALSLLRKTGFEFIEIDDADIVELNQTLTFTVPLYEFFADLAQSLLSLGWKHGIHHIFAQVD
DANVKGIINHHLGEGAIKPAHYLSSLQNGELLKRKMDELFARHNIELLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLAS
NAMLPSITIPVGPEGRLPVGLSFDALRGRDALLLSRVSAIEQVLER*
Nucleotide sequence of regulator: 1341 bp .
>REG0335 NZ_CP008742:c1090918-1089578 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGCATGAAATAATCACACTTGAATCGCTGTGCCAGGCATTGGCTGATGGCGAGATTGCCGCTGCGGAGCTGCGCGAGCG
TGCGCTCGATACTGAGGCCCGCTTGGCCCGTTTGAACTGTTTCATACGTGAGGGTGATGCGGTCAGCCAGTTTGGCGAGG
CTGATCATGCAATGAAGGGTACTCCACTCTGGGGAATGCCGGTTTCCTTCAAGGATAATATCTGTGTTCGCGGTTTGCCG
TTGACAGCCGGGACGCGAGGAATGTCCGGTTTCGTATCTGATCAGGATGCTGCGATTGTCAGTCAACTCAGAGCCCTCGG
GGCTGTGGTCGCTGGCAAGAACAACATGCACGAACTCAGTTTCGGAGTGACCTCCATCAATCCTCATTGGGGAACCGTGG
GAAACCCCGTGGCCCCCGGTTATTGTGCCGGAGGTAGTAGTGGTGGAAGTGCCGCCGCAGTGGCAAGTGGAATTGTTCCG
CTGTCGGTGGGGACCGACACGGGGGGCTCGATAAGAATACCGGCGGCCTTCTGCGGCATTACGGGCTTCAGACCCACTAC
TGGACGCTGGTCAACGGCAGGCATTATCCCTGTTTCTCATACAAAGGATTGCGTTGGGTTGCTGACACGAACGGCAGGCG
ACGCCGGATTTTTGTACGGACTGTTGTCAGGCAAGCAGCAGTCTTTTCCCCTGAGCAGGACGGCCCCCTGTCGAATCGGC
CTACCGGTCTCCATGTGGTCCGATCTGGATGGCGAGGTGGAGAGGGCATGCGTGAATGCACTCAGCCTGCTGCGCAAGAC
AGGGTTCGAGTTTATTGAAATTGATGATGCCGATATTGTCGAACTGAACCAGACACTCACGTTCACCGTTCCGCTTTACG
AATTCTTTGCCGACCTTGCTCAGTCATTGCTCTCCTTGGGCTGGAAGCACGGTATCCATCATATTTTTGCACAGGTTGAT
GATGCCAACGTGAAAGGCATCATCAATCACCACCTTGGGGAGGGCGCTATAAAACCGGCTCACTATTTGAGTTCACTGCA
AAATGGCGAGCTGTTGAAAAGAAAAATGGATGAGCTATTTGCTCGTCATAATATCGAGCTCCTGGGGTACCCCACGGTAC
CTTGCCGGGTACCTCATCTGGATCATGCTGACCGACCAGAGTTTTTCAGTCAGGCAATTCGCAATACCGACCTGGCCAGC
AATGCGATGCTCCCCTCCATTACTATTCCTGTCGGGCCAGAAGGGCGATTGCCGGTCGGCTTGAGCTTTGATGCTCTAAG
AGGGCGAGACGCACTTTTGCTGAGCAGAGTCAGTGCCATAGAGCAGGTATTAGAACGTTGA
ATGCATGAAATAATCACACTTGAATCGCTGTGCCAGGCATTGGCTGATGGCGAGATTGCCGCTGCGGAGCTGCGCGAGCG
TGCGCTCGATACTGAGGCCCGCTTGGCCCGTTTGAACTGTTTCATACGTGAGGGTGATGCGGTCAGCCAGTTTGGCGAGG
CTGATCATGCAATGAAGGGTACTCCACTCTGGGGAATGCCGGTTTCCTTCAAGGATAATATCTGTGTTCGCGGTTTGCCG
TTGACAGCCGGGACGCGAGGAATGTCCGGTTTCGTATCTGATCAGGATGCTGCGATTGTCAGTCAACTCAGAGCCCTCGG
GGCTGTGGTCGCTGGCAAGAACAACATGCACGAACTCAGTTTCGGAGTGACCTCCATCAATCCTCATTGGGGAACCGTGG
GAAACCCCGTGGCCCCCGGTTATTGTGCCGGAGGTAGTAGTGGTGGAAGTGCCGCCGCAGTGGCAAGTGGAATTGTTCCG
CTGTCGGTGGGGACCGACACGGGGGGCTCGATAAGAATACCGGCGGCCTTCTGCGGCATTACGGGCTTCAGACCCACTAC
TGGACGCTGGTCAACGGCAGGCATTATCCCTGTTTCTCATACAAAGGATTGCGTTGGGTTGCTGACACGAACGGCAGGCG
ACGCCGGATTTTTGTACGGACTGTTGTCAGGCAAGCAGCAGTCTTTTCCCCTGAGCAGGACGGCCCCCTGTCGAATCGGC
CTACCGGTCTCCATGTGGTCCGATCTGGATGGCGAGGTGGAGAGGGCATGCGTGAATGCACTCAGCCTGCTGCGCAAGAC
AGGGTTCGAGTTTATTGAAATTGATGATGCCGATATTGTCGAACTGAACCAGACACTCACGTTCACCGTTCCGCTTTACG
AATTCTTTGCCGACCTTGCTCAGTCATTGCTCTCCTTGGGCTGGAAGCACGGTATCCATCATATTTTTGCACAGGTTGAT
GATGCCAACGTGAAAGGCATCATCAATCACCACCTTGGGGAGGGCGCTATAAAACCGGCTCACTATTTGAGTTCACTGCA
AAATGGCGAGCTGTTGAAAAGAAAAATGGATGAGCTATTTGCTCGTCATAATATCGAGCTCCTGGGGTACCCCACGGTAC
CTTGCCGGGTACCTCATCTGGATCATGCTGACCGACCAGAGTTTTTCAGTCAGGCAATTCGCAATACCGACCTGGCCAGC
AATGCGATGCTCCCCTCCATTACTATTCCTGTCGGGCCAGAAGGGCGATTGCCGGTCGGCTTGAGCTTTGATGCTCTAAG
AGGGCGAGACGCACTTTTGCTGAGCAGAGTCAGTGCCATAGAGCAGGTATTAGAACGTTGA