Detailed information of regulator


Summary

Regulator ID REG0334 experimental
Regulator type protein
Regulatory pathway (from literatures) IAA regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Tryptophan metabolism (map00380)
Related T6SS T6SS01041 insolico (Type i4b)
Strain Pseudomonas savastanoi pv. savastanoi NCPPB 3335
Replicon chromosome
Sequence Protein sequence (558 a.a.); Nucleotide sequence (1674 bp)
Description The transcript levels of vgrG doubled in the presence of IAA, indicating a positive effect of IAA on the expression of the T6SS in Psv. A similar effect on the transcript levels of T6SS genes has been reported in Azospirillum brasilense by the addition of exogenous IAA.
Reference
External database links

Locus tag (Gene) PSA3335_RS05390 (iaaM)
Coordinates (Strand) 1090961..1092634 (-)
NCBI ID 488619197
RefSeq NZ_CP008742
Uniprot ID TR2M_PSESS, A0A0Q0B337_PSESS
KEGG ID ag:AAA25852
KEGG pathway (determined by BlastKOALA) map01100; map00380
PDB ID 4IV9
  Target
Target Regulation (↓/↑) Target sequence
The transcript levels of vgrG ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Amino_oxidase PF01593.26 1.8e-99 48..535
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-557 MYDHFNSPSIDILYDYGPFLKKCEMTGGIGSYSAGTPTPRVAIVGAGISGLVAATELLRAGVKDVVLYESRDRIGGRVWSQVFDQTRPRYIAEMGAMRFPPSATGLFHYLKKFGISTSTTFPDPGVVDTELHYRGKRYHWPAGKKPPELFRRVYEGWQSLLSEGYLLEGGSLVAPLDITAMLKSGRLEEAAIAWQGWLNVFRDCSFYNAIVCIFTGRHPPGGDRWARPEDFELFGSLGIGSGGFLPVFQAGFTEILRMVINGYQSDQRLIPDGISSLAARLADQSFDGKALRDRVCFSRVGRISREAEKIIIQTEAGEQRVFDRVIVTSSNRAMQMIHCLTDSESFLSRDVARAVRETHLTGSSKLFILTRTKFWIKNKLPTTIQSDGLVRGVYCLDYQPDEPEGHGVVLLSYTWEDDAQKMLAMPDKKTRCQVLVDDLAAIHPTFASYLLPVDGDYERYVLHHDWLTDPHSAGAFKLNYPGEDVYSQRLFFQPMTANSPNKDTGLYLAGCSCSFAGGWIEGAVQTALNSACAVLRSTGGQLSKGNPLDCINASYRY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9199 - -


  Protein sequence of regulator: 558 a.a.    .

>REG0334 NZ_CP008742:c1092634-1090961 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MYDHFNSPSIDILYDYGPFLKKCEMTGGIGSYSAGTPTPRVAIVGAGISGLVAATELLRAGVKDVVLYESRDRIGGRVWS
QVFDQTRPRYIAEMGAMRFPPSATGLFHYLKKFGISTSTTFPDPGVVDTELHYRGKRYHWPAGKKPPELFRRVYEGWQSL
LSEGYLLEGGSLVAPLDITAMLKSGRLEEAAIAWQGWLNVFRDCSFYNAIVCIFTGRHPPGGDRWARPEDFELFGSLGIG
SGGFLPVFQAGFTEILRMVINGYQSDQRLIPDGISSLAARLADQSFDGKALRDRVCFSRVGRISREAEKIIIQTEAGEQR
VFDRVIVTSSNRAMQMIHCLTDSESFLSRDVARAVRETHLTGSSKLFILTRTKFWIKNKLPTTIQSDGLVRGVYCLDYQP
DEPEGHGVVLLSYTWEDDAQKMLAMPDKKTRCQVLVDDLAAIHPTFASYLLPVDGDYERYVLHHDWLTDPHSAGAFKLNY
PGEDVYSQRLFFQPMTANSPNKDTGLYLAGCSCSFAGGWIEGAVQTALNSACAVLRSTGGQLSKGNPLDCINASYRY*

  Nucleotide sequence of regulator: 1674 bp    .

>REG0334 NZ_CP008742:c1092634-1090961 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGTATGACCATTTTAATTCACCCAGTATTGATATTTTGTACGACTACGGTCCCTTTCTGAAAAAATGTGAAATGACGGG
AGGCATAGGCAGCTATTCAGCCGGAACGCCCACCCCTCGGGTAGCGATAGTCGGTGCCGGCATCAGTGGGCTGGTCGCTG
CAACTGAACTATTACGTGCGGGAGTCAAGGACGTTGTCTTATATGAATCGCGTGATCGAATCGGGGGACGGGTATGGTCT
CAAGTTTTCGATCAGACTCGTCCACGTTACATTGCAGAAATGGGTGCGATGCGCTTTCCTCCCAGTGCAACTGGCCTTTT
CCACTACCTGAAAAAGTTTGGTATTTCGACGTCGACCACCTTTCCGGATCCTGGTGTGGTGGACACGGAGCTGCATTACC
GTGGCAAGCGCTATCACTGGCCAGCGGGCAAAAAGCCGCCCGAATTATTCAGGCGAGTCTATGAGGGGTGGCAGTCTCTA
TTGTCCGAAGGTTACCTCCTTGAAGGCGGTTCTTTAGTTGCCCCGCTGGACATTACCGCAATGCTGAAGTCGGGTCGTCT
GGAAGAGGCAGCGATCGCATGGCAGGGATGGCTCAATGTATTCCGGGATTGTTCATTCTATAACGCGATTGTCTGTATTT
TTACTGGCCGCCATCCGCCAGGCGGCGACAGATGGGCTCGTCCTGAAGACTTTGAGCTGTTTGGCTCGCTTGGCATAGGC
TCGGGCGGGTTTTTGCCAGTCTTTCAGGCTGGCTTTACGGAAATACTGCGGATGGTTATCAACGGATACCAAAGTGACCA
GCGACTGATTCCGGACGGGATATCCAGTCTGGCCGCGAGACTCGCTGATCAGTCGTTTGACGGCAAAGCGTTAAGGGACC
GCGTTTGTTTTAGCCGGGTAGGTCGCATTTCCAGAGAAGCTGAAAAAATCATCATCCAGACGGAAGCAGGAGAACAGCGT
GTATTTGATCGAGTAATTGTCACTAGCAGTAATCGGGCCATGCAAATGATTCACTGCCTCACGGATAGCGAGAGCTTTCT
GAGTCGTGATGTCGCTCGTGCTGTCCGCGAAACCCATCTGACAGGTTCATCGAAGCTTTTCATTCTCACCCGAACCAAAT
TCTGGATAAAAAACAAGCTTCCCACCACCATCCAGTCGGACGGTCTGGTGCGCGGCGTCTATTGTCTGGATTATCAGCCC
GATGAACCTGAGGGGCATGGCGTTGTTCTGCTCAGTTACACGTGGGAAGACGACGCTCAAAAAATGCTGGCGATGCCTGA
CAAGAAAACGCGTTGCCAGGTACTGGTTGATGACCTTGCTGCGATACACCCGACGTTCGCCAGTTATCTCCTGCCCGTTG
ATGGGGATTATGAGCGGTATGTATTGCACCATGACTGGCTCACCGATCCCCATTCTGCGGGCGCTTTCAAACTCAATTAT
CCCGGCGAGGACGTTTACTCGCAGCGATTGTTTTTTCAACCAATGACAGCGAACAGTCCCAATAAAGACACGGGGCTCTA
TCTGGCTGGCTGCAGTTGCTCTTTTGCCGGAGGGTGGATCGAAGGTGCTGTCCAGACAGCATTGAACAGTGCTTGCGCGG
TGCTGCGCAGCACCGGAGGGCAACTGTCAAAAGGCAACCCGCTGGACTGTATCAACGCCTCCTATCGCTATTAA