Detailed information of regulator


Summary

Regulator ID REG0313 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01112 experimental (Type i1)
Strain Vibrio cholerae strain A1552
Replicon chromosome
Sequence Protein sequence (628 a.a.); Nucleotide sequence (1884 bp)
Description LonA tempers motility and T6SS-dependent killing in V. cholerae by controlling cellular levels of TfoY. LonA regulates T6SS-dependent killing in WT, Δ2PDE, and Δ4DGC strains and that LonA-dependent regulation is most prominent when cellular c-di-GMP levels are low.
Reference
External database links

Locus tag (Gene) VCA1552_RS17835 (cdgD)
Coordinates (Strand) 633312..635195 (+)
NCBI ID 446210820
RefSeq NZ_CP024868
Uniprot ID UPI00005F4684
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
the levels of c-di-GMP ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
GGDEF PF00990.23 1e-36 466..614
PAS_9 PF13426.9 6.1e-14 351..451
dCache_1 PF02743.20 3.2e-05 40..273
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MYTSARKY
Transmembrane helix 9-31 FIQFAIVAFVLGFIPTLYFIHAA
Outside 32-627 SQLETQAVSSVEKQTRLQLEFSQHDLLRMLESTHQATQLLAKNDLLFTAVTTPSKEALSQLKTLWDVTLRSQAIFSSFRLLDRQGKEQLKAIYDGHQVTFVESAQTTDPFSQQIVAQYAQLTTPQVWATQVAMSADTPSGMLPTFRFVTGIEHQGQRQGFLVVTVKLQSLYQRLSFIYDQFDSPDILNSAGELLLSEHKPSGTRSTSSLHFSAQHPELWQKIQLNQQGFALSNQTWFSYIKVDLSSVLPDFKPLVLVLRINKAEIDKTYANARWALMSQAVTVLSLLSIIAAGFAAWNINHLKNSLDSKLARAAMDGMSAVVITDRQNRIIKVNNEFTRLSGYTFEDVKGKQPSIFASGLHKVEFYMQMWKALQDNGVWEGEVINKRKDGESITEILRIQSIRDEDNVIQFYVASFVDISHRKALENRLRELSEKDALTDLWNRRKFDQTISLECAKRRRYPDQAQSCLAIIDIDHFKRINDKFGHNEGDLVLRTVAKGIQDQLRESDFIARIGGEEFAIIFPYTSIEEAEQVLNRVRLHIASLHHQQVTLSGGVTDVCTSPDQSYKRADLALYESKTSGRNQISVLTAMEMHHFA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8155 - -


  Protein sequence of regulator: 628 a.a.    .

>REG0313 NZ_CP024868:633312-635195 [Vibrio cholerae]
MYTSARKYFIQFAIVAFVLGFIPTLYFIHAASQLETQAVSSVEKQTRLQLEFSQHDLLRMLESTHQATQLLAKNDLLFTA
VTTPSKEALSQLKTLWDVTLRSQAIFSSFRLLDRQGKEQLKAIYDGHQVTFVESAQTTDPFSQQIVAQYAQLTTPQVWAT
QVAMSADTPSGMLPTFRFVTGIEHQGQRQGFLVVTVKLQSLYQRLSFIYDQFDSPDILNSAGELLLSEHKPSGTRSTSSL
HFSAQHPELWQKIQLNQQGFALSNQTWFSYIKVDLSSVLPDFKPLVLVLRINKAEIDKTYANARWALMSQAVTVLSLLSI
IAAGFAAWNINHLKNSLDSKLARAAMDGMSAVVITDRQNRIIKVNNEFTRLSGYTFEDVKGKQPSIFASGLHKVEFYMQM
WKALQDNGVWEGEVINKRKDGESITEILRIQSIRDEDNVIQFYVASFVDISHRKALENRLRELSEKDALTDLWNRRKFDQ
TISLECAKRRRYPDQAQSCLAIIDIDHFKRINDKFGHNEGDLVLRTVAKGIQDQLRESDFIARIGGEEFAIIFPYTSIEE
AEQVLNRVRLHIASLHHQQVTLSGGVTDVCTSPDQSYKRADLALYESKTSGRNQISVLTAMEMHHFA*

  Nucleotide sequence of regulator: 1884 bp    .

>REG0313 NZ_CP024868:633312-635195 [Vibrio cholerae]
ATGTACACCTCAGCCCGTAAATATTTCATACAATTTGCCATTGTTGCGTTTGTACTTGGTTTCATTCCTACACTGTATTT
CATACATGCTGCTAGCCAGCTTGAGACTCAAGCGGTCAGCAGCGTTGAAAAACAGACTCGCTTACAGCTTGAGTTCAGTC
AGCATGACTTGTTACGAATGCTGGAAAGCACACACCAAGCCACCCAGCTGTTAGCTAAAAATGACCTTTTATTCACGGCT
GTCACCACACCAAGCAAAGAAGCACTCAGTCAACTCAAAACATTGTGGGATGTGACGTTAAGATCGCAAGCGATTTTCTC
TTCATTCAGATTGCTGGATAGACAAGGAAAAGAACAACTTAAAGCGATTTACGATGGGCACCAAGTCACCTTTGTTGAAT
CTGCTCAAACGACAGATCCGTTCAGCCAGCAAATTGTGGCTCAATACGCCCAACTCACGACGCCTCAAGTTTGGGCAACG
CAAGTCGCGATGTCAGCAGATACGCCTTCTGGTATGCTGCCGACCTTTCGTTTTGTGACGGGTATTGAGCATCAAGGCCA
ACGGCAAGGTTTTCTTGTCGTGACGGTGAAGCTACAGTCTCTCTATCAACGTCTCTCTTTTATTTATGATCAGTTTGATT
CACCGGATATTTTGAATTCGGCAGGAGAATTACTGCTCAGTGAACACAAGCCATCCGGTACACGTTCAACCTCTTCACTC
CACTTTTCAGCCCAACACCCAGAGCTTTGGCAAAAAATCCAACTCAACCAACAAGGCTTTGCTCTATCCAATCAAACCTG
GTTTAGCTATATCAAAGTGGATCTCAGTTCTGTCTTACCTGACTTTAAACCTTTGGTATTGGTACTGCGCATCAATAAGG
CAGAAATAGATAAGACCTACGCAAATGCGCGCTGGGCACTGATGAGTCAAGCGGTGACAGTGTTATCGCTACTCTCTATC
ATTGCGGCTGGATTTGCGGCATGGAACATCAACCATTTAAAAAATAGCCTTGACAGTAAATTGGCTCGAGCAGCGATGGA
TGGCATGTCAGCGGTGGTCATTACCGACCGCCAGAATCGCATCATCAAAGTAAACAACGAATTTACCCGCCTAAGTGGTT
ACACTTTTGAAGATGTCAAAGGTAAGCAGCCGTCCATTTTTGCTTCTGGATTACACAAAGTCGAATTCTATATGCAGATG
TGGAAAGCTCTGCAAGACAATGGCGTATGGGAAGGTGAAGTGATCAACAAACGCAAAGATGGCGAAAGCATCACCGAAAT
TCTCCGTATTCAAAGCATCCGCGATGAAGACAATGTCATTCAATTCTACGTTGCCTCTTTTGTGGATATTTCACATCGCA
AGGCGCTGGAGAATCGCCTGCGTGAGCTGAGCGAAAAAGATGCGTTAACCGATTTGTGGAATCGACGTAAATTCGATCAA
ACCATCTCTTTAGAGTGCGCTAAGCGTCGCCGTTATCCCGATCAAGCCCAGAGCTGCCTTGCTATCATTGATATCGACCA
CTTTAAACGCATTAACGACAAATTCGGACACAACGAAGGGGACCTAGTGTTACGGACCGTTGCGAAAGGCATCCAAGATC
AGTTACGGGAATCGGATTTTATCGCACGGATTGGCGGAGAAGAGTTTGCCATTATTTTCCCCTACACTTCCATTGAAGAA
GCCGAACAAGTACTTAACCGCGTACGCCTGCATATCGCTTCATTACACCATCAACAAGTGACCCTAAGTGGTGGTGTTAC
CGATGTTTGCACATCACCCGACCAAAGCTACAAAAGAGCCGATCTGGCTTTATATGAATCCAAAACATCGGGACGCAACC
AAATATCAGTACTCACCGCCATGGAAATGCATCACTTTGCGTGA