Detailed information of regulator
Summary
| Regulator ID | REG0312  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | c-di-GMP regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Biofilm formation - Vibrio cholerae (map05111) |
| Related T6SS | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (409 a.a.); Nucleotide sequence (1227 bp) |
| Description | LonA tempers motility and T6SS-dependent killing in V. cholerae by controlling cellular levels of TfoY. LonA regulates T6SS-dependent killing in WT, Δ2PDE, and Δ4DGC strains and that LonA-dependent regulation is most prominent when cellular c-di-GMP levels are low. |
| Reference |
External database links
| Locus tag (Gene) | VCA1552_RS12850 (cdgJ) |
| Coordinates (Strand) | 2733319..2734545 (-) |
| NCBI ID | 446210986 |
| RefSeq | NZ_CP024867 |
| Uniprot ID | A0A0H3AJ37_VIBC3, A0A0K9UQD4_VIBCL, A0A0H3Q9V7_VIBCL, A0A0H6D5B8_VIBCL |
| KEGG ID | vco:VC0395_A2383, vcq:EN18_16885 |
| KEGG pathway (determined by BlastKOALA) | map05111 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| the levels of c-di-GMP | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| EAL | PF00563.22 | 3.9e-14 | 72..194 |
| HDOD | PF08668.14 | 5.3e-14 | 208..324 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-408 | MYTTYVARQPILNAKRHTLGYELLFRDGEKNAFPEYMDADRATYRLIVENFLSLGTNPRIARSRCFINFPHKSLIRRLPLTLPREQIVVEILETCQPTDDLFEAVQELSQRGYLLALDDFVYSPAWERFLPYVQIVKIDIMAMGLDKACEFVRGRLAQGSRRRFLAERVETEDEFHQARHAGFTFFQGYFFSKPEIIKQRYVSPEHVIAMQLFREVCQPEVDYVRVERLVAQDIALSYKLLRFVNTMSDRISVSISSFRQALVYLGQDKLRIFVSLAVASYISSKKPKELYNLSLQRAQFCQLMATHTHFKAHREQAFLIGMFSVLDALLDTSIEQLVEQLPLADDVKLALREREGPLGTLLDLEECFEKADWQGVEQHCLELGFDLEDVRQELIEAQRWSQDINRLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9929 | - | - |
Protein sequence of regulator: 409 a.a. .
>REG0312 NZ_CP024867:c2734545-2733319 [Vibrio cholerae]
MYTTYVARQPILNAKRHTLGYELLFRDGEKNAFPEYMDADRATYRLIVENFLSLGTNPRIARSRCFINFPHKSLIRRLPL
TLPREQIVVEILETCQPTDDLFEAVQELSQRGYLLALDDFVYSPAWERFLPYVQIVKIDIMAMGLDKACEFVRGRLAQGS
RRRFLAERVETEDEFHQARHAGFTFFQGYFFSKPEIIKQRYVSPEHVIAMQLFREVCQPEVDYVRVERLVAQDIALSYKL
LRFVNTMSDRISVSISSFRQALVYLGQDKLRIFVSLAVASYISSKKPKELYNLSLQRAQFCQLMATHTHFKAHREQAFLI
GMFSVLDALLDTSIEQLVEQLPLADDVKLALREREGPLGTLLDLEECFEKADWQGVEQHCLELGFDLEDVRQELIEAQRW
SQDINRLI*
MYTTYVARQPILNAKRHTLGYELLFRDGEKNAFPEYMDADRATYRLIVENFLSLGTNPRIARSRCFINFPHKSLIRRLPL
TLPREQIVVEILETCQPTDDLFEAVQELSQRGYLLALDDFVYSPAWERFLPYVQIVKIDIMAMGLDKACEFVRGRLAQGS
RRRFLAERVETEDEFHQARHAGFTFFQGYFFSKPEIIKQRYVSPEHVIAMQLFREVCQPEVDYVRVERLVAQDIALSYKL
LRFVNTMSDRISVSISSFRQALVYLGQDKLRIFVSLAVASYISSKKPKELYNLSLQRAQFCQLMATHTHFKAHREQAFLI
GMFSVLDALLDTSIEQLVEQLPLADDVKLALREREGPLGTLLDLEECFEKADWQGVEQHCLELGFDLEDVRQELIEAQRW
SQDINRLI*
Nucleotide sequence of regulator: 1227 bp .
>REG0312 NZ_CP024867:c2734545-2733319 [Vibrio cholerae]
ATGTACACAACTTATGTAGCACGCCAACCGATATTGAATGCAAAGCGACACACGCTAGGTTATGAGCTGCTGTTTCGAGA
TGGAGAAAAAAACGCGTTTCCTGAGTACATGGATGCCGATCGTGCTACCTACCGTCTGATTGTTGAAAACTTTTTATCCC
TTGGTACCAACCCGAGAATTGCCCGCTCGCGCTGTTTTATCAACTTTCCCCATAAAAGCTTAATTCGACGTTTGCCGCTG
ACTCTGCCGCGTGAGCAGATCGTGGTAGAAATTTTGGAAACGTGCCAACCGACCGATGATCTTTTTGAGGCGGTACAAGA
GCTGAGTCAGCGAGGTTATCTGCTCGCGTTAGATGATTTTGTGTATTCACCCGCTTGGGAGCGCTTTTTACCCTACGTAC
AGATAGTCAAAATCGACATCATGGCAATGGGGCTCGATAAAGCGTGTGAATTTGTGCGTGGTCGACTGGCTCAGGGAAGT
CGTCGCCGATTTTTGGCAGAACGAGTCGAAACCGAAGATGAGTTTCATCAAGCGCGTCATGCGGGTTTTACCTTCTTCCA
AGGCTATTTTTTCAGTAAGCCAGAAATCATTAAACAGCGCTACGTCAGTCCAGAGCATGTGATTGCCATGCAGCTGTTTC
GCGAAGTCTGTCAGCCTGAAGTCGATTATGTGCGAGTAGAGCGCTTGGTCGCGCAGGACATTGCGCTCTCCTACAAGCTG
CTGCGTTTCGTAAACACTATGTCGGATCGGATCTCGGTCTCCATCTCCTCATTTCGCCAAGCCTTGGTTTATCTTGGACA
GGACAAACTGCGCATTTTCGTCTCTTTGGCGGTCGCGTCTTATATCTCCTCCAAAAAGCCCAAGGAGCTTTACAACTTGT
CGTTACAAAGAGCGCAGTTTTGCCAGTTGATGGCGACGCATACACATTTTAAAGCGCATCGTGAACAAGCGTTTCTGATT
GGCATGTTTTCTGTGCTGGATGCCTTGCTCGATACCTCAATTGAGCAATTGGTGGAGCAACTGCCGCTGGCCGATGATGT
GAAGTTAGCACTGCGAGAGCGTGAAGGGCCACTGGGTACTTTGCTCGATCTTGAGGAATGTTTTGAAAAAGCCGATTGGC
AAGGTGTGGAGCAGCACTGCCTAGAACTGGGTTTTGATCTGGAAGATGTCCGTCAGGAATTGATTGAAGCCCAGCGTTGG
AGCCAGGACATCAATCGATTAATTTAG
ATGTACACAACTTATGTAGCACGCCAACCGATATTGAATGCAAAGCGACACACGCTAGGTTATGAGCTGCTGTTTCGAGA
TGGAGAAAAAAACGCGTTTCCTGAGTACATGGATGCCGATCGTGCTACCTACCGTCTGATTGTTGAAAACTTTTTATCCC
TTGGTACCAACCCGAGAATTGCCCGCTCGCGCTGTTTTATCAACTTTCCCCATAAAAGCTTAATTCGACGTTTGCCGCTG
ACTCTGCCGCGTGAGCAGATCGTGGTAGAAATTTTGGAAACGTGCCAACCGACCGATGATCTTTTTGAGGCGGTACAAGA
GCTGAGTCAGCGAGGTTATCTGCTCGCGTTAGATGATTTTGTGTATTCACCCGCTTGGGAGCGCTTTTTACCCTACGTAC
AGATAGTCAAAATCGACATCATGGCAATGGGGCTCGATAAAGCGTGTGAATTTGTGCGTGGTCGACTGGCTCAGGGAAGT
CGTCGCCGATTTTTGGCAGAACGAGTCGAAACCGAAGATGAGTTTCATCAAGCGCGTCATGCGGGTTTTACCTTCTTCCA
AGGCTATTTTTTCAGTAAGCCAGAAATCATTAAACAGCGCTACGTCAGTCCAGAGCATGTGATTGCCATGCAGCTGTTTC
GCGAAGTCTGTCAGCCTGAAGTCGATTATGTGCGAGTAGAGCGCTTGGTCGCGCAGGACATTGCGCTCTCCTACAAGCTG
CTGCGTTTCGTAAACACTATGTCGGATCGGATCTCGGTCTCCATCTCCTCATTTCGCCAAGCCTTGGTTTATCTTGGACA
GGACAAACTGCGCATTTTCGTCTCTTTGGCGGTCGCGTCTTATATCTCCTCCAAAAAGCCCAAGGAGCTTTACAACTTGT
CGTTACAAAGAGCGCAGTTTTGCCAGTTGATGGCGACGCATACACATTTTAAAGCGCATCGTGAACAAGCGTTTCTGATT
GGCATGTTTTCTGTGCTGGATGCCTTGCTCGATACCTCAATTGAGCAATTGGTGGAGCAACTGCCGCTGGCCGATGATGT
GAAGTTAGCACTGCGAGAGCGTGAAGGGCCACTGGGTACTTTGCTCGATCTTGAGGAATGTTTTGAAAAAGCCGATTGGC
AAGGTGTGGAGCAGCACTGCCTAGAACTGGGTTTTGATCTGGAAGATGTCCGTCAGGAATTGATTGAAGCCCAGCGTTGG
AGCCAGGACATCAATCGATTAATTTAG