Detailed information of regulator


Summary

Regulator ID REG0310 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) Biofilm formation - Vibrio cholerae (map05111)
Related T6SS T6SS01112 experimental (Type i1)
Strain Vibrio cholerae strain A1552
Replicon chromosome
Sequence Protein sequence (674 a.a.); Nucleotide sequence (2022 bp)
Description LonA tempers motility and T6SS-dependent killing in V. cholerae by controlling cellular levels of TfoY. LonA regulates T6SS-dependent killing in WT, Δ2PDE, and Δ4DGC strains and that LonA-dependent regulation is most prominent when cellular c-di-GMP levels are low.
Reference
External database links

Locus tag (Gene) VCA1552_RS04130 (cdgH)
Coordinates (Strand) 861187..863208 (+)
NCBI ID 446848493
RefSeq NZ_CP024867
Uniprot ID UPI00005F3208
KEGG ID -
KEGG pathway (determined by BlastKOALA) map05111
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
the levels of c-di-GMP ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
GGDEF PF00990.23 1.4e-29 513..656
SBP_bac_3 PF00497.22 3.9e-24 256..445
SBP_bac_3 PF00497.22 1.8e-17 46..227
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-489 MLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDVVTRVLFDSIAHHFNLEIEYVNYPSFNDILVAIETGNADFAANITYTDLRAQRFDFSRPTNIEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQQVEYEGHLEALTLLESGRVDGVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLLRESIQKYQLDIRKQALRQSVVESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQACEMLLLKCELVSNGQETWESMFDDLQDKSIDILAPITVSQQRKNLAYFSESYYHPQAILVKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPNKILFSYASQEEKVQALLNKEVDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIKMLNTEQIIHTYDYQPNWRATLLAEKKYQR
Transmembrane helix 490-512 STQWLFAMAFIVLFMVAFYLHGI
Inside 513-673 SHTDNLTKLRNRRALYNRYRRGLSPRLSLVYLDVNTFKSINDQYGHEVGDKVLKQLAQRIEAVWRGRSYRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSFEVSVAIGIAKNRERTESLNEVMHQADIAMYRAKAESTQSPFQAASKVKGLHIV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.8609 pos: 25-26. SLA-AT. MLLNAFSRRVFLWLGWLLISTSSLA


  Protein sequence of regulator: 674 a.a.    .

>REG0310 NZ_CP024867:861187-863208 [Vibrio cholerae]
MLLNAFSRRVFLWLGWLLISTSSLAATSTTYKVATEADDVVTRVLFDSIAHHFNLEIEYVNYPSFNDILVAIETGNADFA
ANITYTDLRAQRFDFSRPTNIEYTYLYSYGGLRLPELRLVGIPKGTTYGTLLKEHYPYIQQVEYEGHLEALTLLESGRVD
GVVDAINQLKPMLLKGLDVQLLNDQLPIQPVSIVTPKGKHSALLGKIEKYAHSAHVQRLLRESIQKYQLDIRKQALRQSV
VESGLNVQRVLRVKLENNPQYALYQPDGSVRGISADVVFQACEMLLLKCELVSNGQETWESMFDDLQDKSIDILAPITVS
QQRKNLAYFSESYYHPQAILVKREHYKDDVYSNVSELVAERIGVIKDDFFEELLQQMLPNKILFSYASQEEKVQALLNKE
VDYIVLNRANFNLLLRESTEMLPIVEDTMIGSFYQYDIAIGFAKNPLGATLAPLFSRAIKMLNTEQIIHTYDYQPNWRAT
LLAEKKYQRSTQWLFAMAFIVLFMVAFYLHGISHTDNLTKLRNRRALYNRYRRGLSPRLSLVYLDVNTFKSINDQYGHEV
GDKVLKQLAQRIEAVWRGRSYRIGGDEFILIGECSAKRLEHVVAQCERFMFVDAERDVSFEVSVAIGIAKNRERTESLNE
VMHQADIAMYRAKAESTQSPFQAASKVKGLHIV*

  Nucleotide sequence of regulator: 2022 bp    .

>REG0310 NZ_CP024867:861187-863208 [Vibrio cholerae]
ATGCTACTTAACGCTTTTTCACGCCGAGTCTTCCTTTGGCTAGGTTGGCTATTGATTTCCACCAGCAGTTTAGCCGCTAC
ATCTACGACGTATAAGGTCGCCACCGAAGCGGATGACGTGGTGACTCGTGTGCTTTTTGATTCGATTGCTCACCACTTCA
ACCTTGAAATTGAATACGTCAACTACCCCAGTTTTAACGATATTCTGGTGGCGATAGAGACTGGCAACGCCGATTTTGCT
GCCAACATTACTTACACTGATTTGCGTGCTCAACGTTTTGATTTTTCAAGACCAACCAACATCGAGTACACCTATCTCTA
CAGTTATGGTGGCCTACGTTTACCCGAGTTGCGCCTCGTGGGTATCCCGAAAGGAACCACCTACGGGACCCTACTAAAAG
AACACTATCCCTATATCCAGCAAGTTGAGTATGAAGGGCATTTAGAAGCGCTCACTTTGCTGGAAAGTGGCCGAGTAGAC
GGAGTGGTTGATGCGATCAATCAGCTCAAACCTATGCTACTGAAAGGGCTTGATGTACAACTCCTTAACGACCAATTACC
GATTCAGCCTGTTTCTATTGTGACGCCTAAAGGCAAACACTCAGCGCTATTGGGCAAGATTGAAAAATACGCGCATTCGG
CTCACGTACAACGTTTATTGCGTGAATCGATCCAAAAGTATCAATTGGACATCCGTAAGCAAGCTCTGCGTCAATCCGTG
GTTGAGAGCGGACTCAACGTGCAGCGTGTATTGCGTGTTAAGCTAGAGAACAACCCGCAATATGCACTTTATCAGCCAGA
CGGTTCGGTTCGTGGGATCAGTGCTGATGTTGTGTTTCAGGCCTGTGAGATGCTACTGCTGAAATGCGAATTGGTCAGTA
ATGGTCAAGAAACATGGGAGAGCATGTTTGATGATTTACAGGATAAAAGCATCGATATTTTGGCTCCTATAACGGTTTCT
CAGCAGCGTAAAAACCTCGCTTACTTCAGTGAAAGCTACTACCACCCACAAGCGATTTTGGTCAAACGTGAACACTATAA
AGACGATGTGTATAGCAATGTGTCTGAGTTGGTGGCTGAACGTATTGGCGTCATCAAAGACGATTTTTTTGAAGAGCTGT
TACAGCAGATGCTGCCGAACAAGATCTTGTTCAGCTACGCAAGTCAGGAAGAGAAAGTTCAAGCCTTACTGAATAAAGAG
GTGGACTACATAGTGCTCAATAGAGCCAATTTTAATCTCTTGCTTCGCGAGTCAACGGAGATGTTACCGATTGTAGAAGA
CACCATGATTGGCAGTTTCTACCAATATGACATTGCGATAGGTTTTGCTAAAAATCCACTTGGTGCAACTCTGGCACCTC
TTTTCTCTCGGGCAATTAAAATGCTCAATACCGAACAGATCATACATACCTATGATTATCAGCCAAATTGGCGAGCCACA
TTACTTGCGGAAAAGAAATATCAGCGCAGTACTCAATGGCTTTTTGCCATGGCTTTCATCGTTTTGTTTATGGTGGCGTT
TTACCTCCATGGCATATCACATACCGATAACCTTACTAAGTTGCGCAATCGTCGCGCTTTGTATAACCGATACCGCCGCG
GGTTATCGCCTCGCCTAAGCTTGGTTTATCTTGACGTGAATACGTTTAAATCAATCAACGATCAGTATGGACATGAAGTG
GGTGACAAAGTCCTTAAGCAGTTGGCTCAGCGCATCGAAGCGGTATGGCGTGGGCGCAGCTATCGGATTGGTGGGGATGA
ATTTATTTTAATCGGTGAATGTTCTGCTAAGCGGCTTGAACATGTGGTTGCGCAATGTGAACGTTTTATGTTTGTGGATG
CAGAGCGCGATGTCAGTTTTGAAGTGAGTGTGGCGATTGGTATTGCTAAGAATCGTGAGCGGACCGAATCACTCAATGAG
GTGATGCACCAAGCGGATATTGCGATGTATCGCGCTAAGGCGGAATCGACGCAATCGCCATTTCAGGCTGCCAGCAAGGT
AAAAGGATTACACATCGTTTAA