Detailed information of regulator
Summary
| Regulator ID | REG0308  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | c-di-GMP regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Biofilm formation - Vibrio cholerae (map05111) |
| Related T6SS | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (506 a.a.); Nucleotide sequence (1518 bp) |
| Description | LonA tempers motility and T6SS-dependent killing in V. cholerae by controlling cellular levels of TfoY. LonA regulates T6SS-dependent killing in WT, Δ2PDE, and Δ4DGC strains and that LonA-dependent regulation is most prominent when cellular c-di-GMP levels are low. |
| Reference |
External database links
| Locus tag (Gene) | VCA1552_RS10420 (cdgL) |
| Coordinates (Strand) | 2215844..2217361 (-) |
| NCBI ID | 446975354 |
| RefSeq | NZ_CP024867 |
| Uniprot ID | Q9KPS7_VIBCH, A0A0H3ANK2_VIBC3, A0A0K9UR18_VIBCL, C3LQ57_VIBCM |
| KEGG ID | vco:VC0395_A1874, vcm:VCM66_2208, vch:VC2285 |
| KEGG pathway (determined by BlastKOALA) | map05111 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| the levels of c-di-GMP | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GGDEF | PF00990.23 | 3.8e-39 | 340..498 |
| CHASE4 | PF05228.15 | 1e-24 | 58..212 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-9 | MNLNNFSLR |
| Transmembrane helix | 10-30 | WLTTLNALAVVLGFLMFYLTF |
| Inside | 31-260 | KYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMADFIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRYMVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNSSVLFKINHHIKHPPFIRTEE |
| Transmembrane helix | 261-283 | ILALLFFSLVMYLVNLWVVIALI |
| Outside | 284-505 | KPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATTINRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVADVLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSLFHQADMALYHAKEHGRDRYVVRNFV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9988 | - | - |
Protein sequence of regulator: 506 a.a. .
>REG0308 NZ_CP024867:c2217361-2215844 [Vibrio cholerae]
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMAD
FIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRY
MVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATT
INRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVAD
VLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
FHQADMALYHAKEHGRDRYVVRNFV*
MNLNNFSLRWLTTLNALAVVLGFLMFYLTFKYFWSHDREVAQVLQLQQAELQRVETLLSLERKAMGASLADYAAWDEMAD
FIAKPTLEFTQSNIGEHAFSSQFLDGVFIYDPEGNLVWGKKYDAATGQSSSYEHLLPDFSRILQQATRLSVDEISTSVRY
MVVEDEPYLAATARVCDSDGKGCNKGFLIFIKKVRAQFANVVEQATGVDIEVLTCKNDAPLPQDEVDVSYIKQLDYSGNS
SVLFKINHHIKHPPFIRTEEILALLFFSLVMYLVNLWVVIALIKPITTASQVLQQFKTSGGKMPDASTFISSEMKEFATT
INRIVGQLEDSQQVLRWQSEHDPLTRISNRRHLEKQLKSYLSDRPQAYLVLFLVDIDFFKRFNDSFGHLAGDEALCSVAD
VLQSVEFHGEKIVARFGGEEFCVVLASDCAFDAEQYAQQMRSKIEQLAIANPVDALCQYLTVSIGGVYAISPKMESYLSL
FHQADMALYHAKEHGRDRYVVRNFV*
Nucleotide sequence of regulator: 1518 bp .
>REG0308 NZ_CP024867:c2217361-2215844 [Vibrio cholerae]
ATGAATTTAAATAACTTTAGCCTACGCTGGCTAACGACACTCAATGCGTTAGCGGTGGTGTTAGGCTTTTTAATGTTTTA
TCTGACCTTTAAATATTTCTGGTCACATGATCGTGAAGTTGCGCAAGTGCTGCAGTTACAACAGGCCGAGTTGCAGCGGG
TTGAAACCTTGTTGTCGTTAGAACGTAAGGCGATGGGGGCTTCTTTGGCAGATTATGCCGCTTGGGATGAAATGGCAGAC
TTCATTGCAAAACCCACCCTTGAGTTCACACAAAGCAATATTGGAGAGCACGCTTTCAGTTCACAATTCCTCGATGGCGT
ATTTATCTATGATCCTGAGGGCAATTTGGTTTGGGGCAAGAAATATGACGCAGCAACGGGGCAGAGCTCTAGCTATGAGC
ACTTATTGCCGGATTTTTCACGTATTTTACAGCAGGCGACACGTTTAAGTGTGGATGAAATCTCGACTTCCGTTCGTTAT
ATGGTGGTGGAGGATGAGCCCTATTTAGCTGCCACGGCTCGTGTCTGTGACAGTGATGGGAAAGGGTGTAACAAAGGTTT
CTTAATCTTTATTAAAAAAGTCCGCGCCCAGTTTGCGAATGTGGTTGAGCAAGCCACTGGTGTCGATATTGAAGTCTTAA
CCTGTAAAAATGATGCGCCCTTACCGCAAGACGAAGTGGATGTTTCATACATCAAGCAACTCGATTACAGCGGTAATTCC
AGTGTGTTGTTTAAAATTAACCATCACATTAAGCATCCTCCCTTCATTCGAACCGAAGAAATTCTTGCTTTGCTGTTCTT
TTCACTGGTGATGTACTTAGTCAATTTATGGGTGGTGATTGCCTTGATCAAACCGATCACGACCGCCAGTCAAGTGTTGC
AACAATTTAAAACATCGGGCGGAAAAATGCCGGATGCGAGTACTTTTATCTCATCGGAGATGAAAGAATTTGCCACTACG
ATCAACCGAATCGTCGGACAATTGGAGGATAGTCAACAAGTGTTACGTTGGCAGTCTGAGCATGATCCACTAACCCGCAT
TTCCAATCGTCGCCATTTAGAAAAGCAGCTTAAAAGTTATTTGAGCGATCGTCCGCAAGCCTATCTGGTGCTGTTTCTTG
TCGATATCGATTTTTTCAAACGTTTCAATGACAGCTTTGGTCATTTGGCTGGCGATGAAGCCTTATGTTCCGTTGCTGAT
GTGCTCCAATCGGTGGAGTTCCATGGCGAGAAAATCGTGGCGCGCTTTGGGGGAGAAGAGTTTTGTGTTGTTTTAGCTTC
GGACTGTGCATTTGATGCAGAACAGTATGCACAACAGATGAGAAGCAAAATCGAGCAGCTCGCGATTGCAAATCCGGTTG
ATGCACTCTGCCAATATCTAACGGTGAGCATTGGTGGTGTGTATGCTATTTCTCCAAAGATGGAGTCTTATCTCTCTTTG
TTCCATCAGGCGGATATGGCACTTTATCATGCGAAAGAACACGGACGAGATCGATACGTTGTGCGAAATTTTGTCTAA
ATGAATTTAAATAACTTTAGCCTACGCTGGCTAACGACACTCAATGCGTTAGCGGTGGTGTTAGGCTTTTTAATGTTTTA
TCTGACCTTTAAATATTTCTGGTCACATGATCGTGAAGTTGCGCAAGTGCTGCAGTTACAACAGGCCGAGTTGCAGCGGG
TTGAAACCTTGTTGTCGTTAGAACGTAAGGCGATGGGGGCTTCTTTGGCAGATTATGCCGCTTGGGATGAAATGGCAGAC
TTCATTGCAAAACCCACCCTTGAGTTCACACAAAGCAATATTGGAGAGCACGCTTTCAGTTCACAATTCCTCGATGGCGT
ATTTATCTATGATCCTGAGGGCAATTTGGTTTGGGGCAAGAAATATGACGCAGCAACGGGGCAGAGCTCTAGCTATGAGC
ACTTATTGCCGGATTTTTCACGTATTTTACAGCAGGCGACACGTTTAAGTGTGGATGAAATCTCGACTTCCGTTCGTTAT
ATGGTGGTGGAGGATGAGCCCTATTTAGCTGCCACGGCTCGTGTCTGTGACAGTGATGGGAAAGGGTGTAACAAAGGTTT
CTTAATCTTTATTAAAAAAGTCCGCGCCCAGTTTGCGAATGTGGTTGAGCAAGCCACTGGTGTCGATATTGAAGTCTTAA
CCTGTAAAAATGATGCGCCCTTACCGCAAGACGAAGTGGATGTTTCATACATCAAGCAACTCGATTACAGCGGTAATTCC
AGTGTGTTGTTTAAAATTAACCATCACATTAAGCATCCTCCCTTCATTCGAACCGAAGAAATTCTTGCTTTGCTGTTCTT
TTCACTGGTGATGTACTTAGTCAATTTATGGGTGGTGATTGCCTTGATCAAACCGATCACGACCGCCAGTCAAGTGTTGC
AACAATTTAAAACATCGGGCGGAAAAATGCCGGATGCGAGTACTTTTATCTCATCGGAGATGAAAGAATTTGCCACTACG
ATCAACCGAATCGTCGGACAATTGGAGGATAGTCAACAAGTGTTACGTTGGCAGTCTGAGCATGATCCACTAACCCGCAT
TTCCAATCGTCGCCATTTAGAAAAGCAGCTTAAAAGTTATTTGAGCGATCGTCCGCAAGCCTATCTGGTGCTGTTTCTTG
TCGATATCGATTTTTTCAAACGTTTCAATGACAGCTTTGGTCATTTGGCTGGCGATGAAGCCTTATGTTCCGTTGCTGAT
GTGCTCCAATCGGTGGAGTTCCATGGCGAGAAAATCGTGGCGCGCTTTGGGGGAGAAGAGTTTTGTGTTGTTTTAGCTTC
GGACTGTGCATTTGATGCAGAACAGTATGCACAACAGATGAGAAGCAAAATCGAGCAGCTCGCGATTGCAAATCCGGTTG
ATGCACTCTGCCAATATCTAACGGTGAGCATTGGTGGTGTGTATGCTATTTCTCCAAAGATGGAGTCTTATCTCTCTTTG
TTCCATCAGGCGGATATGGCACTTTATCATGCGAAAGAACACGGACGAGATCGATACGTTGTGCGAAATTTTGTCTAA