Detailed information of regulator


Summary

Regulator ID REG0307 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01112 experimental (Type i1)
Strain Vibrio cholerae strain A1552
Replicon chromosome
Sequence Protein sequence (550 a.a.); Nucleotide sequence (1650 bp)
Description LonA tempers motility and T6SS-dependent killing in V. cholerae by controlling cellular levels of TfoY. LonA regulates T6SS-dependent killing in WT, Δ2PDE, and Δ4DGC strains and that LonA-dependent regulation is most prominent when cellular c-di-GMP levels are low.
Reference
External database links

Locus tag (Gene) VCA1552_RS06160 (lonA)
Coordinates (Strand) 1315238..1316887 (+)
NCBI ID 446116573
RefSeq NZ_CP024867
Uniprot ID UPI000218FC66
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
VCA1552_01510 (TfoY) ↑ 446282733
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
AAA_32 PF13654.8 4.9e-19 79..287
Lon_C PF05362.15 2e-10 400..504
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-549 MTQSIWRSVTPQFEQFNKVFENYPNLAPISLIEIQPRLTSALEQFVHLKGFSRILLINAPDNSIYRSLFKDALLEQDLDVPVVSTETLDISEILGQVKVEHGGLVHKTSGYLEQANGGYLIVSANLLLANPRYWPTVKAAVLGETVSPINCDPKSPLPNRLGSSYDVKLIVVGDRTQLGDLDFLDADIHSGLCLFSELELDLKIEEETIEDYLGYLRWLCEQYNLPSLTCSAIQSLMTAGARYTEDQYYAPLCLMWHRALLEEARLESEEPLIDAEHIEIALDKRYLRESYLPERALDDILDGQVIIETQGEQVGQVNGLTVIEVTGHPMPYGEPARISCVIHFGDGDIADVERKVELGGNLHAKGMMIMQAFVSSALNLDGSLPFSASIVFEQSYSEVDGDSASLAELCSLVSALSEYPIDQQIAVTGAVDQFGRVQAVGGLNEKIEGFYRVCCHQGLTGEQGVILPSSNLKHLALHKSVVESIKNGEFNIWPVSTVDEAIPLLMGKPFRGEDEDSVIAKIAERIDNFEKLVQPHGIVERIKNWLSWH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9969 - -


  Protein sequence of regulator: 550 a.a.    .

>REG0307 NZ_CP024867:1315238-1316887 [Vibrio cholerae]
MTQSIWRSVTPQFEQFNKVFENYPNLAPISLIEIQPRLTSALEQFVHLKGFSRILLINAPDNSIYRSLFKDALLEQDLDV
PVVSTETLDISEILGQVKVEHGGLVHKTSGYLEQANGGYLIVSANLLLANPRYWPTVKAAVLGETVSPINCDPKSPLPNR
LGSSYDVKLIVVGDRTQLGDLDFLDADIHSGLCLFSELELDLKIEEETIEDYLGYLRWLCEQYNLPSLTCSAIQSLMTAG
ARYTEDQYYAPLCLMWHRALLEEARLESEEPLIDAEHIEIALDKRYLRESYLPERALDDILDGQVIIETQGEQVGQVNGL
TVIEVTGHPMPYGEPARISCVIHFGDGDIADVERKVELGGNLHAKGMMIMQAFVSSALNLDGSLPFSASIVFEQSYSEVD
GDSASLAELCSLVSALSEYPIDQQIAVTGAVDQFGRVQAVGGLNEKIEGFYRVCCHQGLTGEQGVILPSSNLKHLALHKS
VVESIKNGEFNIWPVSTVDEAIPLLMGKPFRGEDEDSVIAKIAERIDNFEKLVQPHGIVERIKNWLSWH*

  Nucleotide sequence of regulator: 1650 bp    .

>REG0307 NZ_CP024867:1315238-1316887 [Vibrio cholerae]
ATGACCCAAAGCATCTGGCGCAGTGTAACGCCTCAATTTGAGCAGTTTAACAAAGTCTTTGAAAACTACCCTAACCTAGC
TCCCATTTCACTGATCGAGATTCAGCCTCGTTTAACCAGTGCGCTTGAACAGTTTGTTCACCTTAAAGGGTTTAGTCGAA
TCCTCTTGATTAATGCTCCCGATAACTCGATTTATCGCTCTCTCTTTAAAGACGCATTACTTGAGCAGGATCTTGATGTT
CCTGTCGTGAGTACAGAGACGTTAGATATTTCAGAGATTCTAGGCCAAGTCAAAGTTGAGCATGGTGGTCTAGTTCATAA
AACGTCAGGATATCTCGAACAAGCGAATGGCGGTTATTTGATTGTTTCAGCGAATTTATTGCTGGCTAATCCACGCTATT
GGCCAACAGTAAAAGCCGCCGTTTTGGGTGAAACTGTCTCTCCTATCAATTGCGATCCAAAATCTCCGCTCCCTAATCGC
TTAGGTTCAAGTTACGACGTTAAACTTATTGTTGTCGGTGATCGCACGCAGCTTGGCGATCTGGATTTTTTAGATGCTGA
TATTCATTCCGGCTTATGTTTGTTTTCAGAGCTTGAATTAGATCTCAAGATAGAAGAAGAAACGATTGAAGACTACCTCG
GCTACTTACGCTGGTTGTGTGAACAGTACAATTTGCCTTCATTAACCTGCTCTGCCATCCAGTCTCTGATGACGGCCGGT
GCTCGTTATACTGAAGATCAATATTACGCTCCCCTTTGTTTAATGTGGCATCGAGCGCTACTTGAAGAAGCTCGCCTTGA
ATCTGAAGAGCCGTTGATTGACGCTGAACATATTGAAATTGCTTTAGATAAACGCTATCTGCGCGAGTCTTATTTACCCG
AAAGAGCTCTAGACGACATTCTTGACGGTCAAGTGATCATCGAAACCCAGGGTGAACAAGTCGGGCAAGTTAATGGCTTG
ACAGTCATCGAAGTCACTGGGCACCCAATGCCTTACGGTGAGCCAGCTCGAATTTCCTGTGTGATCCATTTTGGTGATGG
TGACATTGCGGATGTAGAGAGAAAAGTTGAGTTAGGCGGCAACCTTCATGCTAAAGGTATGATGATTATGCAAGCTTTTG
TGAGTAGTGCCTTGAATCTTGATGGTTCTCTGCCCTTTTCTGCTTCGATTGTTTTTGAGCAGTCTTATAGTGAAGTTGAT
GGTGATAGCGCTTCACTGGCGGAATTATGTTCATTAGTGAGTGCGCTTTCTGAGTATCCGATTGACCAACAGATTGCCGT
TACTGGCGCTGTTGACCAATTTGGACGAGTTCAAGCGGTCGGTGGTCTGAATGAAAAAATTGAAGGATTTTATCGCGTCT
GTTGTCACCAAGGCTTAACTGGAGAACAGGGGGTGATTTTGCCAAGTTCGAACCTGAAACACTTGGCGCTTCATAAATCA
GTAGTAGAAAGCATTAAGAATGGTGAATTCAACATCTGGCCTGTTTCGACGGTGGATGAAGCGATTCCTCTTCTCATGGG
TAAACCTTTCCGTGGAGAAGATGAAGACAGCGTGATCGCAAAGATCGCTGAAAGAATTGATAATTTTGAAAAACTTGTGC
AACCGCACGGAATTGTGGAACGGATCAAAAACTGGCTTAGCTGGCACTGA