Detailed information of regulator
Summary
| Regulator ID | REG0306  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | c-di-GMP regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (196 a.a.); Nucleotide sequence (588 bp) |
| Description | TfoY-mediated increase in T6SS gene expression coincided with interbacterial killing |
| Reference |
External database links
| Locus tag (Gene) | VCA1552_01510 (TfoY) |
| Coordinates (Strand) | 1636980..1637567 (+) |
| NCBI ID | 446282733 |
| RefSeq | NZ_CP024867 |
| Uniprot ID | Q9KRC0_VIBCH, A0A0X1KXG0_VIBCO, A0A0H3PZM2_VIBCL, A0A0H6P8L8_VIBCL |
| KEGG ID | vch:VC1722, vcq:EN18_11795 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TfoX_C | PF04994.15 | 1.5e-32 | 115..194 |
| TfoX_N | PF04993.15 | 6.5e-30 | 12..103 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-195 | MDKPVLKDSMRLFEQLGRVKSRSMFGGFGIFVDETMFALVVNDTLHIRADDATIEKYKQQGYEPYVYKKRGFPVVTKYYALPEDCWSHPDSILNEVRAALEVAKAERETQAQAKPDRLKDLPNLRLATERMLKKAGIDTVESLQTLGSVEAYKAVQRTHTAEVSLELLWALEGAIEGKHWSVIPQNRRDELLRHL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9881 | - | - |
Protein sequence of regulator: 196 a.a. .
>REG0306 NZ_CP024867:1636980-1637567 [Vibrio cholerae]
MDKPVLKDSMRLFEQLGRVKSRSMFGGFGIFVDETMFALVVNDTLHIRADDATIEKYKQQGYEPYVYKKRGFPVVTKYYA
LPEDCWSHPDSILNEVRAALEVAKAERETQAQAKPDRLKDLPNLRLATERMLKKAGIDTVESLQTLGSVEAYKAVQRTHT
AEVSLELLWALEGAIEGKHWSVIPQNRRDELLRHL*
MDKPVLKDSMRLFEQLGRVKSRSMFGGFGIFVDETMFALVVNDTLHIRADDATIEKYKQQGYEPYVYKKRGFPVVTKYYA
LPEDCWSHPDSILNEVRAALEVAKAERETQAQAKPDRLKDLPNLRLATERMLKKAGIDTVESLQTLGSVEAYKAVQRTHT
AEVSLELLWALEGAIEGKHWSVIPQNRRDELLRHL*
Nucleotide sequence of regulator: 588 bp .
>REG0306 NZ_CP024867:1636980-1637567 [Vibrio cholerae]
ATGGATAAACCAGTATTAAAAGATTCAATGCGGCTCTTTGAGCAGCTAGGACGTGTTAAATCTCGTTCAATGTTTGGTGG
CTTTGGTATTTTCGTCGACGAAACTATGTTTGCGCTTGTGGTTAACGATACGCTGCACATCCGTGCAGATGACGCCACAA
TCGAAAAGTATAAACAGCAAGGTTATGAACCTTATGTTTACAAAAAACGTGGCTTCCCCGTTGTAACTAAGTACTACGCT
CTACCTGAAGATTGTTGGAGTCATCCAGACTCTATCCTCAATGAAGTGCGTGCAGCACTGGAGGTCGCTAAGGCCGAACG
TGAAACTCAAGCACAAGCGAAACCTGATCGTTTGAAAGATCTTCCTAACTTGCGTTTAGCAACAGAAAGAATGCTCAAAA
AAGCAGGTATCGACACCGTCGAGTCTCTTCAGACTCTTGGTTCTGTCGAAGCTTATAAAGCGGTTCAACGAACCCACACG
GCAGAGGTGAGTTTAGAGTTGCTATGGGCTCTAGAAGGCGCAATCGAAGGTAAACATTGGTCGGTGATCCCACAAAATCG
TCGTGATGAGCTATTAAGGCATCTCTGA
ATGGATAAACCAGTATTAAAAGATTCAATGCGGCTCTTTGAGCAGCTAGGACGTGTTAAATCTCGTTCAATGTTTGGTGG
CTTTGGTATTTTCGTCGACGAAACTATGTTTGCGCTTGTGGTTAACGATACGCTGCACATCCGTGCAGATGACGCCACAA
TCGAAAAGTATAAACAGCAAGGTTATGAACCTTATGTTTACAAAAAACGTGGCTTCCCCGTTGTAACTAAGTACTACGCT
CTACCTGAAGATTGTTGGAGTCATCCAGACTCTATCCTCAATGAAGTGCGTGCAGCACTGGAGGTCGCTAAGGCCGAACG
TGAAACTCAAGCACAAGCGAAACCTGATCGTTTGAAAGATCTTCCTAACTTGCGTTTAGCAACAGAAAGAATGCTCAAAA
AAGCAGGTATCGACACCGTCGAGTCTCTTCAGACTCTTGGTTCTGTCGAAGCTTATAAAGCGGTTCAACGAACCCACACG
GCAGAGGTGAGTTTAGAGTTGCTATGGGCTCTAGAAGGCGCAATCGAAGGTAAACATTGGTCGGTGATCCCACAAAATCG
TCGTGATGAGCTATTAAGGCATCTCTGA