Detailed information of regulator


Summary

Regulator ID REG0295 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01112 experimental (Type i1)
Strain Vibrio cholerae strain A1552
Replicon chromosome
Sequence Protein sequence (93 a.a.); Nucleotide sequence (279 bp)
Description Direct VCA0117‐mediated regulation of Hcp is dependent on RpoN (σ54) and IHF, consistent with the findings above, analysis of wild‐type V. cholerae A1552 and its isogenic IHF‐null and RpoN (σ54) derivatives showed that lack of either σ54 or IHF lead to undetectable levels of Hcp.
Reference
External database links

Locus tag (Gene) VCA1552_RS08565 (ihfB)
Coordinates (Strand) 1839353..1839631 (-)
NCBI ID 446089486
RefSeq NZ_CP024867
Uniprot ID A0A067B9D6_VIBMT, IHFB_VIBCH, A0A0X1KXY1_VIBCO, IHFB_VIBC3, D7H9X7_VIBCL, A0A0K9UZN0_VIBCL, A0A0H3Q019_VIBCL, IHFB_VIBCM, A0A1X1LIF6_VIBCL, D0IKS3_9VIBR
KEGG ID vco:VC0395_A1504, vcr:VC395_2029, vcz:VAB027_2558, vcq:EN18_12690, vcx:VAA049_961, vcm:VCM66_1838, vch:VC1914
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Hcp secretion ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Bac_DNA_binding PF00216.23 1.9e-30 1..91
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-92 MTKSELIERLCAEQTHLSAKEIEDAVKNILEHMASTLEAGERIEIRGFGSFSLHYREPRVGRNPKTGDKVELEGKYVPHFKPGKELRERVNL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9875 - -


  Protein sequence of regulator: 93 a.a.    .

>REG0295 NZ_CP024867:c1839631-1839353 [Vibrio cholerae]
MTKSELIERLCAEQTHLSAKEIEDAVKNILEHMASTLEAGERIEIRGFGSFSLHYREPRVGRNPKTGDKVELEGKYVPHF
KPGKELRERVNL*

  Nucleotide sequence of regulator: 279 bp    .

>REG0295 NZ_CP024867:c1839631-1839353 [Vibrio cholerae]
ATGACTAAGTCCGAACTGATAGAAAGACTCTGTGCCGAACAAACGCACTTGTCTGCGAAAGAGATCGAAGATGCGGTAAA
AAATATCCTTGAGCATATGGCCTCAACGTTAGAAGCGGGGGAGCGCATCGAGATTCGTGGTTTCGGTAGCTTTTCCTTAC
ACTATCGTGAGCCGCGTGTAGGGCGTAATCCTAAGACTGGCGATAAGGTGGAGTTGGAAGGTAAGTACGTTCCGCATTTC
AAACCAGGTAAGGAACTACGCGAACGCGTTAACTTGTAA