SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0293
Regulator type	protein
Regulatory pathway (from literatures)	Global regulator
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111)
Related T6SS	T6SS01112 (Type i1)
Strain	Vibrio cholerae strain A1552
Replicon	chromosome
Sequence	Protein sequence (204 a.a.); Nucleotide sequence (612 bp)
Description	TfoX-mediated T6SS induction required HapR; The expression of Hcp in the V. cholerae O1 strain was positively regulated by the quorum sensing regulator HapR.
Reference	[1] Metzger LC, Stutzmann S, Scrignari T, et al. Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY. Cell Rep. 2016 May 3;15(5):951-958. doi: 10.1016/j.celrep.2016.03.092. Epub 2016 Apr 21. PMID: 27117415 [2] Ishikawa T, Rompikuntal PK, Lindmark B, et al. Quorum sensing regulation of the two hcp alleles in Vibrio cholerae O1 strains. PLoS One. 2009 Aug 24;4(8):e6734. doi: 10.1371/journal.pone.0006734. PMID: 19701456

External database links

Locus tag (Gene)	VCA1552_00351 (HapR)
Coordinates (Strand)	340447..341058 (-)
NCBI ID	446262243
RefSeq	NZ_CP024867
Uniprot ID	B2CKP3_VIBCL
KEGG ID	vcq:EN18_19905
KEGG pathway (determined by BlastKOALA)	map02020; map02024; map05111
PDB ID	6D7R

Target

Target	Regulation (↓/↑)	Target sequence
Unknown		-
Hcp expression and secretion		-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
TetR_N	PF00440.25	5.2e-09	23..67

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-203	MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFLTDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDVDNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9940	-	-

Protein sequence of regulator: 204 a.a. .

                                    >REG0293 NZ_CP024867:c341058-340447 [Vibrio cholerae]
MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFL
TDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDV
DNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN*

                                 

Nucleotide sequence of regulator: 612 bp .

                                    >REG0293 NZ_CP024867:c341058-340447 [Vibrio cholerae]
ATGGACGCATCAATCGAAAAACGCCCTCGAACTCGGCTATCGCCTCAAAAACGCAAACTACAACTGATGGAAATCGCGTT
GGAAGTGTTTGCTAAACGCGGCATTGGTCGTGGTGGTCACGCAGATATTGCCGAGATTGCGCAAGTCTCCGTTGCAACAG
TGTTCAACTACTTCCCAACTCGTGAAGACTTGGTTGACGATGTGCTGAATTTTGTGGTTCGTCAGTACTCCAACTTCTTG
ACCGATCACATCGATCTTGATTTGGATGTGAAAACCAACCTACAAACTCTGTGCAAAGAGATGGTGAAATTGGCGATGAC
CGATTGTCACTGGCTCAAAGTCTGGTTTGAGTGGAGTGCTTCAACCCGTGACGAAGTTTGGCCACTGTTTGTTTCCACCA
ACCGAACTAACCAACTGCTGATCAGAAACATGTTTATGAAAGCGATGGAGCGTGGCGAATTGTGTGAGAAACACGATGTC
GATAACATGGCCAGCCTGTTCCACGGCATCTTCTACTCCATCTTCTTACAAGTGAACCGTTTAGGTGAACAAGAAGCAGT
GTATAAGTTGGCCGATAGCTACCTCAATATGCTGTGTATCTATAAGAACTAG

                                