Detailed information of regulator
Summary
| Regulator ID | REG0204  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-RR |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020) |
| Related T6SS | T6SS00346 (Type i4b) |
| Strain | Edwardsiella ictaluri 93-146 |
| Replicon | chromosome |
| Sequence | Protein sequence (215 a.a.); Nucleotide sequence (645 bp) |
| Description | the T6SS translocon protein, EvpC, was secreted by ΔesrA::km but not by ΔesrB::km or ΔesrC. |
| Reference |
External database links
| Locus tag (Gene) | NT01EI_RS04325 (EsrB) |
| Coordinates (Strand) | 955101..955745 (-) |
| NCBI ID | 506350644 |
| RefSeq | NC_012779 |
| Uniprot ID | C5BBE4_EDWI9, Q1AC76_EDWIC |
| KEGG ID | eic:NT01EI_0965 |
| KEGG pathway (determined by BlastKOALA) | map02020 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS translocon protein EvpC secretion | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 7.9e-27 | 10..121 |
| GerE | PF00196.21 | 2.7e-18 | 151..205 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-214 | MTISILPLSILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWNDMRIVVVTASSEARRGSDALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLEASSDIAQEPPLTLRERQILKLVAEGNRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9035 | - | - |
Protein sequence of regulator: 215 a.a. .
>REG0204 NC_012779:c955745-955101 [Edwardsiella ictaluri 93-146]
MTISILPLSILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWN
DMRIVVVTASSEARRGSDALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLEASSDIAQEPPLTLRERQIL
KLVAEGNRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEV*
MTISILPLSILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWN
DMRIVVVTASSEARRGSDALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLEASSDIAQEPPLTLRERQIL
KLVAEGNRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEV*
Nucleotide sequence of regulator: 645 bp .
>REG0204 NC_012779:c955745-955101 [Edwardsiella ictaluri 93-146]
ATGACTATCTCTATTTTGCCGCTGAGCATTCTGATCGTTGAAGACCATGCCTTGCTGGCCGATGGCATCCGTAATCTCCT
GGACAGTCAGCCCCGCTACCGGGTCGTGGGCGCCGTATCGAATGGACTGGCCGTTTATGAGGCCTGCCAGCGCCTCTCGC
CCGATCTGGTCTTGCTCGATCTGGGTCTGCCGGGCATGGACGGCGTTGATGTAATCCGTCAGCTGCGGCGCCGCTGGAAC
GATATGCGTATCGTCGTCGTCACCGCCTCGTCTGAGGCGCGGCGCGGCAGCGACGCCCTCGCCGCCGGCGCGCTCGCCTA
TGTTCTGAAGCAAAGCCCACAGCAAACCCTGCTGGCAGCAATGCAGCATGCATCACTGGGAAAAACCTTTGTCGATCCCG
CCCTCAGGCTGGACTCCCTGGAGGCCTCTTCCGATATAGCTCAGGAACCGCCCCTGACCCTGCGCGAACGGCAAATACTC
AAGCTGGTCGCCGAGGGTAATCGCAACCGGGATATCGCTGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCATCG
GCTCAACCTGATGCGCAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAATTGGGCCCGCCGACTGGGTGTGCTGGAGG
TTTAA
ATGACTATCTCTATTTTGCCGCTGAGCATTCTGATCGTTGAAGACCATGCCTTGCTGGCCGATGGCATCCGTAATCTCCT
GGACAGTCAGCCCCGCTACCGGGTCGTGGGCGCCGTATCGAATGGACTGGCCGTTTATGAGGCCTGCCAGCGCCTCTCGC
CCGATCTGGTCTTGCTCGATCTGGGTCTGCCGGGCATGGACGGCGTTGATGTAATCCGTCAGCTGCGGCGCCGCTGGAAC
GATATGCGTATCGTCGTCGTCACCGCCTCGTCTGAGGCGCGGCGCGGCAGCGACGCCCTCGCCGCCGGCGCGCTCGCCTA
TGTTCTGAAGCAAAGCCCACAGCAAACCCTGCTGGCAGCAATGCAGCATGCATCACTGGGAAAAACCTTTGTCGATCCCG
CCCTCAGGCTGGACTCCCTGGAGGCCTCTTCCGATATAGCTCAGGAACCGCCCCTGACCCTGCGCGAACGGCAAATACTC
AAGCTGGTCGCCGAGGGTAATCGCAACCGGGATATCGCTGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCATCG
GCTCAACCTGATGCGCAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAATTGGGCCCGCCGACTGGGTGTGCTGGAGG
TTTAA