Detailed information of regulator


Summary

Regulator ID REG0184 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Flagellar assembly (map02040)
Related T6SS T6SS00216 insolico (Type i1)
Strain Marinomonas sp. MWYL1
Replicon chromosome
Sequence Protein sequence (492 a.a.); Nucleotide sequence (1476 bp)
Description Plasmids constructed transcriptional fusions, in whichβ-glucuronidase, is under the control of the promoter of the T6SS have been introduced into the E. coli strain or its rpoN derivative, and β-glucuronidase activities showed T6SS promotor activites was reduced in absence of rpoN and EMSA assays results the promoter regions of these T6SS gene clusters are under the control of σ54.
Reference
External database links

Locus tag (Gene) Mmwyl1_2418 (RpoN)
Coordinates (Strand) 2725212..2726687 (+)
NCBI ID 501017436
RefSeq NC_009654
Uniprot ID A6VY11_MARMS
KEGG ID mmw:Mmwyl1_2418
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02040
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS gene cluster promoter (PMWYL1196/PMWYL1195) ↓ atagtaaaattccttatttaaaaatagtaaaaacgtgtttttaacactcgccctaggaagagcaagatggatgccaattaaaaatagttaatattttcaatgggttaataaaaacacaccctaccatcgagcaaaacttcgcttggggtcaggcgaaaaatcgcccgacgagcaaaaactcgcttgttaggagtggtgggtaattgctctctagctggagggggaagttgttgttttaaaagaagattgtggctagataaggtgaatctaaaaaaacatcattacgaattatttacatttcctttgccttatcttttgttgagctttttagttttagtgcgtattctatgaaaatattaaatgccattagatgatggcttcgtaatttattagcaaatttttatagcaatggaaggcagttttttaagcaattggagtgttgaagggatttaaactaaacgcaaaggtattatccttgaggggtatttttttgtgttttacatggaagttattattgagtaaatttgttaacttttcctatttccctgcttttttaatattccctttttttaaattttagttttgtttagatttttgatatctagatatggctagggttttttatgctcttttttttgtgtttttcaaagaaagggcgctgtatcgatatttttatatggattttgagtgattttaagttgttatccttaagtggtaatgcagctaaaattgtttttttatgacgtcgttggatggagaaaagta Putative σ54 binding sequence is indicated by red color
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_DBD PF04552.15 7.2e-70 331..489
Sigma54_CBD PF04963.15 1.1e-54 122..316
Sigma54_AID PF00309.22 4.4e-17 4..49
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-491 MKQSLQLKLGQQLTMTPQLQQAIRLLQLSTLDLKQEIHEFLESNPLLELDDDEQIHYDIPASIESKSAADTTVDNNSEEPRVESEWTESIPEELSIDSNWDDTYQSSAAVSDSNSYEGDSDFESRNAPAESIQDHLIWQLNLTHMSDRDIEIAYAIIECVQPDGYLSISPDDICESLSIELEDLELDEVIAVQHRLQRFDPVGVCSIDLRDCLLVQLEAFQSHPLYKSTYNLIDHYLPLLGNRDFAQLSRKTKLKDEALKEVVDLIQQLNPRPGSVIDADDTDYVIPDVTVKKRHDVWQVELNNDALPPIKINETYSAMVSTAAATAEDVTYIKTSLQEAKWFMKSLQSRNETLLKVASCIVSRQIDFFELGEEAMKPMVLHDVAEEVGMHESTISRVTTQKYMHTPKGIFELKYFFSSHVNTASGGECSSTAIRAMIKKLVSDEPAKKPLSDNKLAAILADQGIQVARRTVAKYREAMNIPPSNERKRLI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9880 - -


  Protein sequence of regulator: 492 a.a.    .

>REG0184 NC_009654:2725212-2726687 [Marinomonas sp. MWYL1]
MKQSLQLKLGQQLTMTPQLQQAIRLLQLSTLDLKQEIHEFLESNPLLELDDDEQIHYDIPASIESKSAADTTVDNNSEEP
RVESEWTESIPEELSIDSNWDDTYQSSAAVSDSNSYEGDSDFESRNAPAESIQDHLIWQLNLTHMSDRDIEIAYAIIECV
QPDGYLSISPDDICESLSIELEDLELDEVIAVQHRLQRFDPVGVCSIDLRDCLLVQLEAFQSHPLYKSTYNLIDHYLPLL
GNRDFAQLSRKTKLKDEALKEVVDLIQQLNPRPGSVIDADDTDYVIPDVTVKKRHDVWQVELNNDALPPIKINETYSAMV
STAAATAEDVTYIKTSLQEAKWFMKSLQSRNETLLKVASCIVSRQIDFFELGEEAMKPMVLHDVAEEVGMHESTISRVTT
QKYMHTPKGIFELKYFFSSHVNTASGGECSSTAIRAMIKKLVSDEPAKKPLSDNKLAAILADQGIQVARRTVAKYREAMN
IPPSNERKRLI*

  Nucleotide sequence of regulator: 1476 bp    .

>REG0184 NC_009654:2725212-2726687 [Marinomonas sp. MWYL1]
ATGAAGCAGTCTTTACAATTAAAGCTTGGTCAACAGCTGACTATGACGCCACAACTGCAACAGGCTATTCGCTTGCTGCA
GCTTTCTACGTTGGATTTAAAACAAGAAATTCATGAATTCCTTGAATCGAATCCCCTGCTTGAGCTTGACGACGACGAAC
AAATTCATTACGACATCCCAGCAAGCATTGAATCAAAAAGCGCTGCCGATACAACAGTCGACAACAACAGCGAAGAACCA
CGCGTAGAATCTGAGTGGACTGAAAGCATCCCTGAAGAACTATCCATAGATAGTAACTGGGATGATACCTATCAAAGTTC
CGCCGCTGTCAGCGATAGCAACAGCTATGAAGGCGATAGCGACTTCGAAAGCCGTAACGCACCAGCAGAAAGCATCCAAG
ACCATCTTATATGGCAATTAAATTTAACCCACATGTCAGATCGCGACATTGAAATCGCTTACGCCATCATTGAATGCGTG
CAGCCAGACGGCTATCTAAGCATTTCGCCTGATGATATTTGCGAATCTCTAAGTATTGAATTAGAAGACTTAGAATTAGA
TGAAGTCATTGCAGTGCAACATAGATTGCAACGCTTTGACCCTGTTGGTGTGTGCTCAATCGATCTACGTGACTGCCTTC
TTGTACAACTAGAAGCATTCCAAAGCCACCCGCTGTATAAGAGCACATACAATCTTATTGATCATTATCTACCATTATTA
GGTAATCGAGATTTTGCTCAGTTGAGCCGTAAGACAAAACTAAAAGACGAAGCCCTAAAAGAAGTCGTCGATTTGATTCA
ACAGCTCAACCCACGCCCAGGCTCAGTTATCGACGCTGACGACACGGATTACGTTATTCCAGATGTCACAGTGAAAAAAC
GCCATGATGTATGGCAAGTGGAGCTAAACAACGATGCTTTGCCGCCCATCAAAATCAATGAGACCTACTCAGCGATGGTT
AGCACCGCAGCAGCAACAGCAGAAGACGTGACTTACATAAAAACCTCTCTTCAAGAAGCAAAATGGTTCATGAAGAGCCT
ACAGAGCCGCAACGAAACTTTATTAAAAGTGGCGAGCTGTATTGTAAGTCGACAAATTGATTTCTTCGAATTAGGCGAAG
AAGCCATGAAACCCATGGTTCTTCACGACGTTGCAGAAGAAGTAGGCATGCATGAATCAACTATTTCCAGGGTAACAACA
CAGAAATACATGCATACGCCGAAAGGCATCTTTGAGCTGAAATACTTTTTCTCCAGCCATGTAAACACTGCCTCTGGCGG
AGAATGTTCTTCTACCGCAATACGAGCTATGATTAAAAAGCTTGTGTCAGATGAACCGGCGAAAAAACCGTTAAGCGATA
ACAAGCTAGCTGCTATTTTAGCAGACCAAGGCATACAGGTTGCTCGACGCACTGTAGCCAAATATCGAGAGGCGATGAAC
ATCCCTCCTTCCAATGAACGAAAACGACTGATCTAA