Detailed information of regulator


Summary

Regulator ID REG0181 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00208 insolico (Type i1)
Strain Vibrio cholerae O395
Replicon chromosome
Sequence Protein sequence (531 a.a.); Nucleotide sequence (1593 bp)
Description The reporter fusion assays results that the bEBPs bind to the T6SS gene cluster promoters to activate their transcription and EMSA assays results that bEBPs specifically bind the promoter region of their cognate T6SS gene cluster.
Reference
External database links

Locus tag (Gene) VC0395_0020/VCA0117 (bEBP/vasH)
Coordinates (Strand) 22315..23907 (-)
NCBI ID 446006938
RefSeq NC_009456
Uniprot ID VASH_VIBCH, A0A0X1L1T0_VIBCO, A0A0H3AFG8_VIBC3, A0A0K9UVH8_VIBCL, A0A0H3QAP0_VIBCL, C3LUE1_VIBCM, A0A023PRF8_VIBCL
KEGG ID vcm:VCM66_A0115, vch:VCA0117, vcq:EN18_04310, vco:VC0395_0020, vcx:VAA049_1791
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS gene cluster promoter (PVCA0107/VC0395_RS00160) ↓ catattacgtctccaatacctatgccaaacgttgtcaatgaaacagttgattgaaatcattcaatcaaccaagaatcttgaacttatccttagtgataactaagggctctcatttcaaaatatgaaatagagtcaatcacttccgtgacactgtgagaaaataaatcccacactaagccatgtcacagagtgttgttgtattttgatatattgatgaaaaatgggcttattatgcaaacttatcattagcagctttttgctgccatgtatcagttatctggtataaatattgtttgcggtgatctaatacccaataaatatgagatggcattctattcatggagacaaaaaagtaatcgaaaaaaaacccgaagcgtgatcttttaccc
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 1.5e-68 193..359
HTH_8 PF02954.21 1.8e-06 486..521
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-530 MSQWLAFATQLVGVRKSHQLALQFVDLLTQGLDLSDSLLLLPSSDGRLLVPHDPQRQFAWSVTDFDVPFAHVLQSSNAMHLTAEELVFWQSNRTFSQLTSRVGMFDSVWIQPLPMDTRQVHSILLLMGESHGIVSAFENADFLKFIEVFSQQWSLLNDMEREEQRRLELKQSLTDIERDSAQRSLANALSRTLIGESAAMQKLREQIVSAANSQLSVMVQGETGTGKELVAAAVHELSSRKSAPFVAINCAAIPEHLLESELFGYCKGAFSGADSDKQGLIAQANGGTLFLDEIGDMPLTLQAKLLRVLESRTFRPLGGKQELSSDFRLVSATHVNLLDQVRKKEFRQDLYYRLFQYPITLPRLAARLEDIELLSEHFVRVFNLQHNTRIRGLNYRAIDCLKQYDFPGNVRELKHLIEFGCAQTADGTQVEASCFAHRLQTLPCLAPEATPVAVSVETENVDLEPSVALAGEPNFAVIHDLKQAVSQFEALIISERLNRFAGDRAKAAKSLGIPKRTLAYKCLKLEIKTP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9730 - -


  Protein sequence of regulator: 531 a.a.    .

>REG0181 NC_009456:c23907-22315 [Vibrio cholerae O395]
MSQWLAFATQLVGVRKSHQLALQFVDLLTQGLDLSDSLLLLPSSDGRLLVPHDPQRQFAWSVTDFDVPFAHVLQSSNAMH
LTAEELVFWQSNRTFSQLTSRVGMFDSVWIQPLPMDTRQVHSILLLMGESHGIVSAFENADFLKFIEVFSQQWSLLNDME
REEQRRLELKQSLTDIERDSAQRSLANALSRTLIGESAAMQKLREQIVSAANSQLSVMVQGETGTGKELVAAAVHELSSR
KSAPFVAINCAAIPEHLLESELFGYCKGAFSGADSDKQGLIAQANGGTLFLDEIGDMPLTLQAKLLRVLESRTFRPLGGK
QELSSDFRLVSATHVNLLDQVRKKEFRQDLYYRLFQYPITLPRLAARLEDIELLSEHFVRVFNLQHNTRIRGLNYRAIDC
LKQYDFPGNVRELKHLIEFGCAQTADGTQVEASCFAHRLQTLPCLAPEATPVAVSVETENVDLEPSVALAGEPNFAVIHD
LKQAVSQFEALIISERLNRFAGDRAKAAKSLGIPKRTLAYKCLKLEIKTP*

  Nucleotide sequence of regulator: 1593 bp    .

>REG0181 NC_009456:c23907-22315 [Vibrio cholerae O395]
ATGAGTCAATGGCTGGCGTTTGCAACCCAATTGGTGGGAGTAAGGAAGTCCCACCAGCTTGCGCTGCAATTTGTTGATCT
GCTGACTCAAGGCTTAGATCTGAGTGATAGCCTTTTGTTGTTGCCATCGTCGGATGGGCGTTTACTTGTGCCTCATGATC
CACAGCGTCAGTTCGCGTGGTCAGTGACGGATTTTGATGTGCCTTTTGCCCATGTTTTGCAGTCATCCAATGCGATGCAT
TTGACCGCGGAAGAGTTGGTATTTTGGCAATCCAATCGCACTTTTAGCCAGTTAACCTCTCGCGTGGGGATGTTTGATTC
GGTATGGATCCAACCCCTGCCGATGGATACTCGTCAAGTACACTCGATCTTGCTGTTGATGGGCGAGAGTCACGGTATCG
TGAGCGCTTTTGAAAACGCGGACTTTTTGAAGTTTATCGAGGTTTTTTCCCAGCAATGGTCACTGCTCAACGATATGGAA
CGTGAAGAGCAGCGCCGCTTGGAGCTGAAACAATCGCTGACGGATATTGAACGCGACTCGGCGCAGCGCTCACTGGCCAA
TGCGTTATCGCGTACTTTGATTGGTGAAAGCGCTGCGATGCAAAAGCTGCGTGAGCAGATTGTCTCTGCCGCTAACTCTC
AGCTCTCGGTCATGGTGCAAGGTGAAACTGGCACGGGGAAAGAGTTAGTCGCCGCGGCCGTGCATGAACTTTCGTCACGT
AAGTCTGCGCCGTTTGTTGCGATCAACTGTGCGGCGATCCCTGAGCATCTACTGGAAAGTGAACTGTTTGGTTACTGCAA
AGGGGCATTTTCTGGAGCGGACAGCGATAAACAGGGACTTATCGCACAAGCGAATGGTGGCACGCTATTTCTGGATGAGA
TCGGCGATATGCCGCTCACCTTACAAGCCAAATTATTGCGTGTGCTTGAATCGCGTACTTTTCGACCGCTCGGTGGTAAG
CAGGAGCTCAGTTCCGATTTCCGTCTGGTATCTGCCACACACGTCAATCTGCTGGATCAAGTACGCAAAAAAGAATTTCG
CCAAGATCTCTATTACCGTCTATTCCAGTATCCGATCACCTTGCCGCGTTTGGCGGCACGCTTGGAAGATATTGAGCTGC
TCAGTGAGCACTTTGTGCGCGTGTTCAACTTGCAACATAACACGCGCATTCGTGGTTTGAACTATCGAGCCATCGACTGC
CTTAAGCAGTACGACTTCCCGGGAAATGTGCGTGAGCTGAAACATTTGATTGAGTTTGGCTGCGCGCAAACAGCGGATGG
TACGCAGGTGGAAGCTAGTTGCTTTGCCCATCGTTTACAAACCCTACCCTGTCTTGCGCCTGAAGCGACGCCGGTAGCCG
TTTCTGTCGAAACTGAGAACGTGGATCTTGAACCCTCGGTTGCGCTGGCTGGAGAACCGAATTTTGCGGTTATCCATGAT
TTGAAACAGGCGGTCAGTCAGTTTGAAGCGTTGATCATCAGTGAGCGTTTGAACCGCTTTGCTGGCGATCGCGCGAAAGC
GGCGAAAAGTCTCGGTATCCCTAAGCGTACCTTGGCCTACAAGTGCCTGAAATTGGAGATCAAAACCCCATGA