Detailed information of regulator
Summary
| Regulator ID | REG0146  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-RR |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (246 a.a.); Nucleotide sequence (738 bp) |
| Description | Low Molecular Weight Thiols Upregulate T6SS1 Expression through the VirA Sensor.Use Burkholderia pseudomallei K96243 gene description as Burkholderia pseudomallei strain KHW gene annotation. |
| Reference | [1] Wong J, Chen Y, Gan YH. Host Cytosolic Glutathione Sensing by a Membrane Histidine Kinase Activates the Type VI Secretion System in an Intracellular Bacterium. Cell Host Microbe. 2015 Jul 8;18(1):38-48. doi: 10.1016/j.chom.2015.06.002. Epub 2015 Jun 18. PMID: 26094804 [2] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170 |
External database links
| Locus tag (Gene) | BPSS1494 (virG) |
| Coordinates (Strand) | 2037977..2038714 (-) |
| NCBI ID | 53722516 |
| RefSeq | NC_006351 |
| Uniprot ID | Q63K71_BURPS |
| KEGG ID | bps:BPSS1494 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| hcp1 expression | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Trans_reg_C | PF00486.30 | 4.4e-20 | 161..235 |
| Response_reg | PF00072.26 | 7.4e-20 | 10..118 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-245 | MTNATRSPAVLVVEDDEPLRRVLCNYLSGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRETQRFPLICVTGRSEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSTTPVRRGRHPRAYRFDGWTLNMNTRRLLTRDAQEIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKDPKHPELIRTERGVGYYFDAKEVETLWG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8795 | - | - |
Protein sequence of regulator: 246 a.a. .
>REG0146 NC_006351:c2038714-2037977 [Burkholderia pseudomallei K96243]
MTNATRSPAVLVVEDDEPLRRVLCNYLSGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRETQRFP
LICVTGRSEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSTTPVRRGRHPRAYRFDGWTLNMNTRRLLTRDA
QEIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKDPKHPELIRTERGVGYYFDAKEV
ETLWG*
MTNATRSPAVLVVEDDEPLRRVLCNYLSGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRETQRFP
LICVTGRSEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSTTPVRRGRHPRAYRFDGWTLNMNTRRLLTRDA
QEIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKDPKHPELIRTERGVGYYFDAKEV
ETLWG*
Nucleotide sequence of regulator: 738 bp .
>REG0146 NC_006351:c2038714-2037977 [Burkholderia pseudomallei K96243]
ATGACGAATGCGACAAGATCGCCGGCGGTGCTCGTCGTCGAGGACGACGAACCGTTGCGGCGCGTGCTTTGCAATTACCT
GTCGGGCCACGGGATGGCCGTGGACGGCGTGGGAACGGCGGCAGCGGCCGCCGAATCGGTCGGGCGGCGCGAATACGAGA
TGGTGTTGCTCGATCTCGGCCTGCCGGACGGCGACGGCATCGACGTGCTGCTGCGCTGGCGCGAAACGCAGCGCTTTCCG
CTCATCTGTGTGACGGGGCGCAGCGAAGAGGCCGATCGCATCATGGGCCTCGAATTCGGCGCGGACGATTACGTCGTGAA
GCCGTACTCGCTGCGCGAGGTGCTCGCCCGGATGCGCGCGCTGTGGCGGCGCGCCGAGCCGTCGACGACGCCCGTGCGGC
GCGGCAGGCATCCGCGTGCGTACCGCTTCGACGGATGGACGCTGAACATGAACACGCGCCGGCTGTTGACGCGCGATGCG
CAGGAAATTCCGCTGACGGTCGGCGAATTCAACCTGCTCGCGTTCTTTCTCGGCTCGCCGTCGCGCGTCGTCACGCGCGC
GCAGTTGCTCGAGTGCACGCGTGCGTTCGACGACGTGTTCGATCGCGCGGTCGACGTGCAGATCTGGCGGCTGCGCAAGA
AAATCGAGAAGGATCCGAAGCATCCCGAACTGATTCGAACCGAGCGGGGCGTCGGCTACTACTTCGACGCGAAAGAGGTG
GAGACGCTATGGGGATGA
ATGACGAATGCGACAAGATCGCCGGCGGTGCTCGTCGTCGAGGACGACGAACCGTTGCGGCGCGTGCTTTGCAATTACCT
GTCGGGCCACGGGATGGCCGTGGACGGCGTGGGAACGGCGGCAGCGGCCGCCGAATCGGTCGGGCGGCGCGAATACGAGA
TGGTGTTGCTCGATCTCGGCCTGCCGGACGGCGACGGCATCGACGTGCTGCTGCGCTGGCGCGAAACGCAGCGCTTTCCG
CTCATCTGTGTGACGGGGCGCAGCGAAGAGGCCGATCGCATCATGGGCCTCGAATTCGGCGCGGACGATTACGTCGTGAA
GCCGTACTCGCTGCGCGAGGTGCTCGCCCGGATGCGCGCGCTGTGGCGGCGCGCCGAGCCGTCGACGACGCCCGTGCGGC
GCGGCAGGCATCCGCGTGCGTACCGCTTCGACGGATGGACGCTGAACATGAACACGCGCCGGCTGTTGACGCGCGATGCG
CAGGAAATTCCGCTGACGGTCGGCGAATTCAACCTGCTCGCGTTCTTTCTCGGCTCGCCGTCGCGCGTCGTCACGCGCGC
GCAGTTGCTCGAGTGCACGCGTGCGTTCGACGACGTGTTCGATCGCGCGGTCGACGTGCAGATCTGGCGGCTGCGCAAGA
AAATCGAGAAGGATCCGAAGCATCCCGAACTGATTCGAACCGAGCGGGGCGTCGGCTACTACTTCGACGCGAAAGAGGTG
GAGACGCTATGGGGATGA