Detailed information of regulator
Summary
| Regulator ID | REG0144  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | bspR-dependent signalling cascade |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (354 a.a.); Nucleotide sequence (1062 bp) |
| Description | In the ΔbprC mutant, there was no transcript from the tssA promoter as expected, but the hcp1 promoter was still functional. Only tssA was not expressed whereas the other two downstream genes (hcp1 and vgrG) were still highly expressed in the ΔbprC mutant. |
| Reference |
External database links
| Locus tag (Gene) | BPSS1520 (bprC) |
| Coordinates (Strand) | 2072125..2073186 (-) |
| NCBI ID | 499518995 |
| RefSeq | NC_006351 |
| Uniprot ID | Q63K46_BURPS, A0A2K9D6R9_BURPE |
| KEGG ID | bps:BPSS1520 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| tssA expression | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| AraC_binding_2 | PF14525.8 | 2.4e-20 | 53..227 |
| HTH_18 | PF12833.9 | 5.3e-18 | 267..346 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-353 | MRHAFRTDSRPTRFPSHHSPALTMAKMHAPRGGTLLQFFSTDDMPLARAAAFWSAHVFQCEDVRASSARAFHGHGFLCRCERGRFVRFRGASLDTRIGAAWLSAAPADAYVTICALHAGECTVEAPGLPDVRFRANELFMLDGGQPMRVRWSEPCFSALRLPRASVGRTLGQAAMDASPSAASLQEARLAPFLAAELALIGGRGPTLSSDELDYMLARAAELGRTLLQAALSSRARRGAPARADRLQAAYRYIEQHLHLPTLTPERIADAIHCSRTQLYRLFRHESQTVKAALREARLNRSLGYLEQPEVTLSIGEIAHACGFPDQSTFGKLFRRRFGRTPGEVRRAARGCSI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9430 | - | - |
Protein sequence of regulator: 354 a.a. .
>REG0144 NC_006351:c2073186-2072125 [Burkholderia pseudomallei K96243]
MRHAFRTDSRPTRFPSHHSPALTMAKMHAPRGGTLLQFFSTDDMPLARAAAFWSAHVFQCEDVRASSARAFHGHGFLCRC
ERGRFVRFRGASLDTRIGAAWLSAAPADAYVTICALHAGECTVEAPGLPDVRFRANELFMLDGGQPMRVRWSEPCFSALR
LPRASVGRTLGQAAMDASPSAASLQEARLAPFLAAELALIGGRGPTLSSDELDYMLARAAELGRTLLQAALSSRARRGAP
ARADRLQAAYRYIEQHLHLPTLTPERIADAIHCSRTQLYRLFRHESQTVKAALREARLNRSLGYLEQPEVTLSIGEIAHA
CGFPDQSTFGKLFRRRFGRTPGEVRRAARGCSI*
MRHAFRTDSRPTRFPSHHSPALTMAKMHAPRGGTLLQFFSTDDMPLARAAAFWSAHVFQCEDVRASSARAFHGHGFLCRC
ERGRFVRFRGASLDTRIGAAWLSAAPADAYVTICALHAGECTVEAPGLPDVRFRANELFMLDGGQPMRVRWSEPCFSALR
LPRASVGRTLGQAAMDASPSAASLQEARLAPFLAAELALIGGRGPTLSSDELDYMLARAAELGRTLLQAALSSRARRGAP
ARADRLQAAYRYIEQHLHLPTLTPERIADAIHCSRTQLYRLFRHESQTVKAALREARLNRSLGYLEQPEVTLSIGEIAHA
CGFPDQSTFGKLFRRRFGRTPGEVRRAARGCSI*
Nucleotide sequence of regulator: 1062 bp .
>REG0144 NC_006351:c2073186-2072125 [Burkholderia pseudomallei K96243]
ATGCGACATGCGTTCCGCACGGATTCTCGCCCAACGCGCTTCCCGTCGCACCATTCACCGGCCCTCACCATGGCGAAGAT
GCACGCGCCCCGCGGCGGCACGCTGCTGCAATTCTTTTCGACCGACGACATGCCGCTCGCGCGCGCGGCGGCGTTCTGGA
GCGCGCACGTGTTCCAGTGCGAGGACGTGCGCGCGTCGTCCGCGCGCGCGTTTCACGGGCACGGCTTTCTCTGCCGCTGC
GAGCGCGGCCGGTTCGTTCGCTTTCGCGGCGCGTCGCTCGACACGCGCATCGGCGCGGCGTGGCTGAGTGCCGCGCCAGC
CGATGCGTACGTGACGATCTGCGCGCTGCATGCGGGCGAGTGCACGGTCGAAGCGCCCGGCTTGCCGGATGTGCGCTTTC
GTGCGAACGAGCTGTTCATGCTGGACGGCGGGCAGCCGATGCGCGTGCGCTGGAGCGAGCCGTGTTTCAGCGCGCTCAGG
CTGCCGCGCGCATCGGTGGGGCGTACGCTCGGCCAGGCGGCGATGGACGCGTCGCCGAGCGCGGCTTCGTTGCAAGAGGC
GCGGCTCGCGCCGTTTCTCGCGGCGGAGCTCGCGCTGATCGGCGGTCGCGGCCCGACGCTGTCGTCGGACGAGCTCGATT
ACATGCTCGCGCGCGCAGCCGAGCTCGGCCGCACGCTGCTTCAGGCGGCGCTGTCGTCGCGCGCGCGGCGCGGCGCGCCC
GCGCGCGCCGACCGGCTGCAGGCCGCGTATCGCTACATCGAGCAGCATCTTCATCTGCCGACGCTCACACCCGAGCGGAT
CGCCGACGCGATCCATTGCTCGCGCACGCAGCTCTATCGGCTGTTCCGCCATGAATCGCAGACGGTGAAGGCGGCGCTGC
GCGAGGCGCGGCTGAACCGCAGCCTCGGCTATCTCGAGCAGCCCGAGGTTACGCTCAGCATCGGCGAGATCGCGCATGCT
TGCGGTTTTCCCGATCAGTCGACGTTCGGCAAGCTGTTTCGCCGGCGCTTCGGCAGAACGCCCGGCGAGGTGCGCCGTGC
CGCGCGGGGATGTTCGATATGA
ATGCGACATGCGTTCCGCACGGATTCTCGCCCAACGCGCTTCCCGTCGCACCATTCACCGGCCCTCACCATGGCGAAGAT
GCACGCGCCCCGCGGCGGCACGCTGCTGCAATTCTTTTCGACCGACGACATGCCGCTCGCGCGCGCGGCGGCGTTCTGGA
GCGCGCACGTGTTCCAGTGCGAGGACGTGCGCGCGTCGTCCGCGCGCGCGTTTCACGGGCACGGCTTTCTCTGCCGCTGC
GAGCGCGGCCGGTTCGTTCGCTTTCGCGGCGCGTCGCTCGACACGCGCATCGGCGCGGCGTGGCTGAGTGCCGCGCCAGC
CGATGCGTACGTGACGATCTGCGCGCTGCATGCGGGCGAGTGCACGGTCGAAGCGCCCGGCTTGCCGGATGTGCGCTTTC
GTGCGAACGAGCTGTTCATGCTGGACGGCGGGCAGCCGATGCGCGTGCGCTGGAGCGAGCCGTGTTTCAGCGCGCTCAGG
CTGCCGCGCGCATCGGTGGGGCGTACGCTCGGCCAGGCGGCGATGGACGCGTCGCCGAGCGCGGCTTCGTTGCAAGAGGC
GCGGCTCGCGCCGTTTCTCGCGGCGGAGCTCGCGCTGATCGGCGGTCGCGGCCCGACGCTGTCGTCGGACGAGCTCGATT
ACATGCTCGCGCGCGCAGCCGAGCTCGGCCGCACGCTGCTTCAGGCGGCGCTGTCGTCGCGCGCGCGGCGCGGCGCGCCC
GCGCGCGCCGACCGGCTGCAGGCCGCGTATCGCTACATCGAGCAGCATCTTCATCTGCCGACGCTCACACCCGAGCGGAT
CGCCGACGCGATCCATTGCTCGCGCACGCAGCTCTATCGGCTGTTCCGCCATGAATCGCAGACGGTGAAGGCGGCGCTGC
GCGAGGCGCGGCTGAACCGCAGCCTCGGCTATCTCGAGCAGCCCGAGGTTACGCTCAGCATCGGCGAGATCGCGCATGCT
TGCGGTTTTCCCGATCAGTCGACGTTCGGCAAGCTGTTTCGCCGGCGCTTCGGCAGAACGCCCGGCGAGGTGCGCCGTGC
CGCGCGGGGATGTTCGATATGA