Detailed information of regulator


Summary

Regulator ID REG0144 experimental
Regulator type protein
Regulatory pathway (from literatures) bspR-dependent signalling cascade
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00077 insolico (Type i4b)
Strain Burkholderia pseudomallei K96243
Replicon chromosome
Sequence Protein sequence (354 a.a.); Nucleotide sequence (1062 bp)
Description In the ΔbprC mutant, there was no transcript from the tssA promoter as expected, but the hcp1 promoter was still functional. Only tssA was not expressed whereas the other two downstream genes (hcp1 and vgrG) were still highly expressed in the ΔbprC mutant.
Reference
External database links

Locus tag (Gene) BPSS1520 (bprC)
Coordinates (Strand) 2072125..2073186 (-)
NCBI ID 499518995
RefSeq NC_006351
Uniprot ID Q63K46_BURPS, A0A2K9D6R9_BURPE
KEGG ID bps:BPSS1520
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
tssA expression↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
AraC_binding_2PF14525.82.4e-2053..227
HTH_18PF12833.95.3e-18267..346
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-353MRHAFRTDSRPTRFPSHHSPALTMAKMHAPRGGTLLQFFSTDDMPLARAAAFWSAHVFQCEDVRASSARAFHGHGFLCRCERGRFVRFRGASLDTRIGAAWLSAAPADAYVTICALHAGECTVEAPGLPDVRFRANELFMLDGGQPMRVRWSEPCFSALRLPRASVGRTLGQAAMDASPSAASLQEARLAPFLAAELALIGGRGPTLSSDELDYMLARAAELGRTLLQAALSSRARRGAPARADRLQAAYRYIEQHLHLPTLTPERIADAIHCSRTQLYRLFRHESQTVKAALREARLNRSLGYLEQPEVTLSIGEIAHACGFPDQSTFGKLFRRRFGRTPGEVRRAARGCSI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9430--


  Protein sequence of regulator: 354 a.a.    .

>REG0144 NC_006351:c2073186-2072125 [Burkholderia pseudomallei K96243]
MRHAFRTDSRPTRFPSHHSPALTMAKMHAPRGGTLLQFFSTDDMPLARAAAFWSAHVFQCEDVRASSARAFHGHGFLCRC
ERGRFVRFRGASLDTRIGAAWLSAAPADAYVTICALHAGECTVEAPGLPDVRFRANELFMLDGGQPMRVRWSEPCFSALR
LPRASVGRTLGQAAMDASPSAASLQEARLAPFLAAELALIGGRGPTLSSDELDYMLARAAELGRTLLQAALSSRARRGAP
ARADRLQAAYRYIEQHLHLPTLTPERIADAIHCSRTQLYRLFRHESQTVKAALREARLNRSLGYLEQPEVTLSIGEIAHA
CGFPDQSTFGKLFRRRFGRTPGEVRRAARGCSI*

  Nucleotide sequence of regulator: 1062 bp    .

>REG0144 NC_006351:c2073186-2072125 [Burkholderia pseudomallei K96243]
ATGCGACATGCGTTCCGCACGGATTCTCGCCCAACGCGCTTCCCGTCGCACCATTCACCGGCCCTCACCATGGCGAAGAT
GCACGCGCCCCGCGGCGGCACGCTGCTGCAATTCTTTTCGACCGACGACATGCCGCTCGCGCGCGCGGCGGCGTTCTGGA
GCGCGCACGTGTTCCAGTGCGAGGACGTGCGCGCGTCGTCCGCGCGCGCGTTTCACGGGCACGGCTTTCTCTGCCGCTGC
GAGCGCGGCCGGTTCGTTCGCTTTCGCGGCGCGTCGCTCGACACGCGCATCGGCGCGGCGTGGCTGAGTGCCGCGCCAGC
CGATGCGTACGTGACGATCTGCGCGCTGCATGCGGGCGAGTGCACGGTCGAAGCGCCCGGCTTGCCGGATGTGCGCTTTC
GTGCGAACGAGCTGTTCATGCTGGACGGCGGGCAGCCGATGCGCGTGCGCTGGAGCGAGCCGTGTTTCAGCGCGCTCAGG
CTGCCGCGCGCATCGGTGGGGCGTACGCTCGGCCAGGCGGCGATGGACGCGTCGCCGAGCGCGGCTTCGTTGCAAGAGGC
GCGGCTCGCGCCGTTTCTCGCGGCGGAGCTCGCGCTGATCGGCGGTCGCGGCCCGACGCTGTCGTCGGACGAGCTCGATT
ACATGCTCGCGCGCGCAGCCGAGCTCGGCCGCACGCTGCTTCAGGCGGCGCTGTCGTCGCGCGCGCGGCGCGGCGCGCCC
GCGCGCGCCGACCGGCTGCAGGCCGCGTATCGCTACATCGAGCAGCATCTTCATCTGCCGACGCTCACACCCGAGCGGAT
CGCCGACGCGATCCATTGCTCGCGCACGCAGCTCTATCGGCTGTTCCGCCATGAATCGCAGACGGTGAAGGCGGCGCTGC
GCGAGGCGCGGCTGAACCGCAGCCTCGGCTATCTCGAGCAGCCCGAGGTTACGCTCAGCATCGGCGAGATCGCGCATGCT
TGCGGTTTTCCCGATCAGTCGACGTTCGGCAAGCTGTTTCGCCGGCGCTTCGGCAGAACGCCCGGCGAGGTGCGCCGTGC
CGCGCGGGGATGTTCGATATGA