Detailed information of regulator


Summary

Regulator ID REG0137 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR; BsaN regulon
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00077 insolico (Type i4b)
Strain Burkholderia pseudomallei K96243
Replicon chromosome
Sequence Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Description Use SOLiD RNA sequencing technology to assess T6SS-1 transcript levels in B. pseudomallei AI harboring pBHR2 and pBHR2-virAG and expression for each gene in T6SS-1 yielded significant differences in expression when compared directly between AI(pBHR2) and AI(pBHR2-virAG).
Reference
External database links

Locus tag (Gene) BPSS1495 (virA)
Coordinates (Strand) 2038711..2040555 (-)
NCBI ID 499518988
RefSeq NC_006351
Uniprot ID Q63K70_BURPS
KEGG ID bps:BPSS1495
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS-1 transpript ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 3.1e-18 454..592
MASE1 PF05231.16 5.2e-14 63..323
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-32 MHGNSIDAGPPARARPARITRTARAAPRRADR
Transmembrane helix 33-52 AFAPGGALNAAGLLLLLFYA
Outside 53-66 NHLLSRPGACTSLN
Transmembrane helix 67-86 HTLSAVVWPAPVLGIALLLH
Inside 87-92 CRRPLA
Transmembrane helix 93-115 WWLGAGSLFATLLAAGSLDWVPW
Outside 116-118 HVD
Transmembrane helix 119-141 ALFAAVNVVEAIAGAWLARRYLI
Inside 142-153 AGGGVDTLRGFA
Transmembrane helix 154-176 MFVLLLPLGMAAMHATLAAAILS
Outside 177-190 VWMNDREWLSEWSR
Transmembrane helix 191-213 AYAANALALLVLLPALLTWNARA
Inside 214-224 CGATWSRSRNQ
Transmembrane helix 225-247 LPVLATFGSLCFASIWGYQAEVA
Outside 248-261 RALLALSLSWAALE
Transmembrane helix 262-284 GGLVLASQLNALSAIALIAMTLG
Inside 285-296 GRGPYAWHHDGH
Transmembrane helix 297-319 GVWALQVDLIGVAILSMCIAIAT
Outside 320-614 GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8347 - -


  Protein sequence of regulator: 615 a.a.    .

>REG0137 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243]
MHGNSIDAGPPARARPARITRTARAAPRRADRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGATWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT*

  Nucleotide sequence of regulator: 1845 bp    .

>REG0137 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243]
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCGCCGCGCC
GCGCCGCGCCGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTGTGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCGT
GCGGCGCGACGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTCGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTTGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ACCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAACGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGTCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGTGGGCAGTTGCTTTCG
GTTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA