Detailed information of regulator
Summary
| Regulator ID | REG0136  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | BsaN regulon |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (67 a.a.); Nucleotide sequence (201 bp) |
| Description | Plasmids with bprP under arabinose induction were introduced and BprP activated both the bsaM and bsaN promoters in E. coli by measuring β-galactosidase activity. |
| Reference |
External database links
| Locus tag (Gene) | BPS_RS27175 (bprP) |
| Coordinates (Strand) | 2105881..2106081 (+) |
| NCBI ID | 490293055 |
| RefSeq | NC_006351 |
| Uniprot ID | A0A095JJ24_BURPE |
| KEGG ID | but:X994_5564 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| BPSS1546 (BsaN) | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-66 | MTPLPTATHTQTDAADRSTGREPPKPPASLDEIMRQWLINDFKQKVMFPDPDDPSACPTFDGNGSW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8355 | - | - |
Protein sequence of regulator: 67 a.a. .
>REG0136 NC_006351:2105881-2106081 [Burkholderia pseudomallei K96243]
MTPLPTATHTQTDAADRSTGREPPKPPASLDEIMRQWLINDFKQKVMFPDPDDPSACPTFDGNGSW*
MTPLPTATHTQTDAADRSTGREPPKPPASLDEIMRQWLINDFKQKVMFPDPDDPSACPTFDGNGSW*
Nucleotide sequence of regulator: 201 bp .
>REG0136 NC_006351:2105881-2106081 [Burkholderia pseudomallei K96243]
ATGACCCCGCTGCCAACCGCGACGCACACGCAGACTGACGCCGCCGATCGTTCAACGGGCCGCGAGCCCCCGAAGCCGCC
CGCGTCGCTCGACGAGATCATGAGGCAATGGCTGATCAACGACTTCAAGCAGAAGGTGATGTTCCCCGACCCCGACGATC
CGAGCGCCTGCCCGACGTTCGACGGCAACGGCAGTTGGTGA
ATGACCCCGCTGCCAACCGCGACGCACACGCAGACTGACGCCGCCGATCGTTCAACGGGCCGCGAGCCCCCGAAGCCGCC
CGCGTCGCTCGACGAGATCATGAGGCAATGGCTGATCAACGACTTCAAGCAGAAGGTGATGTTCCCCGACCCCGACGATC
CGAGCGCCTGCCCGACGTTCGACGGCAACGGCAGTTGGTGA