Detailed information of regulator
Summary
| Regulator ID | REG0135  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | BsaN regulon |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (172 a.a.); Nucleotide sequence (516 bp) |
| Description | BsaN together with chaperone BicA directly activate T6SS1 regulatory genes, and bsaN/bicA expression constructs resulted in activation of T6SS1 regulatory gene BPSS 1495(virA). |
| Reference |
External database links
| Locus tag (Gene) | BPS_RS27070 (bicA) |
| Coordinates (Strand) | 2087441..2087956 (-) |
| NCBI ID | 490293043 |
| RefSeq | NC_006351 |
| Uniprot ID | A0A0F6L1C9_BURPE, Q63K33_BURPS, A0A069B9Y9_BURPE, B1H8M3_BURPE, C4AWI9_BURML, A3P6Z8_BURP0, A8EPL3_BURPE, A2S1P7_BURM9, A0A0E1S5A7_BURPE, C4I6K4_BURPE, A0A0E1TMP5_BURPE, A0A0E1UN71_BURPE |
| KEGG ID | bpl:BURPS1106A_A2075, bps:BPSS1533, bml:BMA10229_2070, but:X994_5584 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| BPSS 1495(virA) | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TPR_3 | PF07720.14 | 3.5e-11 | 41..74 |
| TPR_3 | PF07720.14 | 7.9e-07 | 76..108 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-171 | MTQRDVNIDDIEAQEMAAALLDAVQNGATLKDLHQVPQDLMDGIYAFAYRFYQQGRLDDAEVFFRFLCIYDFYNAEYAMGLAAVCQLKKEYARAIDLYALAYSLSKDDHRPMFHTGQCHLLMGKAALARRCFGIVVERSRDERLAQKAQSYLDGLDEVGADAAPASAGNDH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9961 | - | - |
Protein sequence of regulator: 172 a.a. .
>REG0135 NC_006351:c2087956-2087441 [Burkholderia pseudomallei K96243]
MTQRDVNIDDIEAQEMAAALLDAVQNGATLKDLHQVPQDLMDGIYAFAYRFYQQGRLDDAEVFFRFLCIYDFYNAEYAMG
LAAVCQLKKEYARAIDLYALAYSLSKDDHRPMFHTGQCHLLMGKAALARRCFGIVVERSRDERLAQKAQSYLDGLDEVGA
DAAPASAGNDH*
MTQRDVNIDDIEAQEMAAALLDAVQNGATLKDLHQVPQDLMDGIYAFAYRFYQQGRLDDAEVFFRFLCIYDFYNAEYAMG
LAAVCQLKKEYARAIDLYALAYSLSKDDHRPMFHTGQCHLLMGKAALARRCFGIVVERSRDERLAQKAQSYLDGLDEVGA
DAAPASAGNDH*
Nucleotide sequence of regulator: 516 bp .
>REG0135 NC_006351:c2087956-2087441 [Burkholderia pseudomallei K96243]
ATGACGCAACGCGACGTGAACATAGACGACATCGAGGCGCAGGAAATGGCGGCGGCGCTGCTGGACGCGGTCCAGAACGG
CGCGACGCTGAAGGACCTGCATCAGGTGCCGCAGGACCTGATGGACGGCATCTATGCGTTCGCGTACCGCTTCTACCAGC
AGGGGCGGCTCGACGACGCGGAGGTGTTCTTCCGCTTTCTGTGCATCTACGACTTCTACAACGCCGAATACGCGATGGGG
CTCGCGGCGGTGTGCCAGTTGAAGAAGGAGTACGCGCGGGCGATCGATCTGTATGCACTCGCGTATTCGCTGTCGAAGGA
CGACCACCGGCCGATGTTCCACACCGGCCAATGCCATCTGCTGATGGGCAAGGCGGCGCTCGCGCGGCGCTGCTTCGGCA
TCGTCGTCGAGCGCTCGCGCGACGAGCGCCTCGCGCAGAAGGCGCAGTCCTATCTCGACGGGCTCGACGAAGTGGGCGCC
GACGCGGCGCCCGCATCCGCCGGGAACGACCACTAG
ATGACGCAACGCGACGTGAACATAGACGACATCGAGGCGCAGGAAATGGCGGCGGCGCTGCTGGACGCGGTCCAGAACGG
CGCGACGCTGAAGGACCTGCATCAGGTGCCGCAGGACCTGATGGACGGCATCTATGCGTTCGCGTACCGCTTCTACCAGC
AGGGGCGGCTCGACGACGCGGAGGTGTTCTTCCGCTTTCTGTGCATCTACGACTTCTACAACGCCGAATACGCGATGGGG
CTCGCGGCGGTGTGCCAGTTGAAGAAGGAGTACGCGCGGGCGATCGATCTGTATGCACTCGCGTATTCGCTGTCGAAGGA
CGACCACCGGCCGATGTTCCACACCGGCCAATGCCATCTGCTGATGGGCAAGGCGGCGCTCGCGCGGCGCTGCTTCGGCA
TCGTCGTCGAGCGCTCGCGCGACGAGCGCCTCGCGCAGAAGGCGCAGTCCTATCTCGACGGGCTCGACGAAGTGGGCGCC
GACGCGGCGCCCGCATCCGCCGGGAACGACCACTAG