Detailed information of regulator
Summary
| Regulator ID | REG0134  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | BsaN regulon |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (222 a.a.); Nucleotide sequence (666 bp) |
| Description | Activation of BsaN regulated T6SS1(BPSS1497/tssB,BPSS1498/hcp) as analyzed by RNAseq. |
| Reference |
External database links
| Locus tag (Gene) | BPSS1546 (BsaN) |
| Coordinates (Strand) | 2100565..2101230 (-) |
| NCBI ID | 490660320 |
| RefSeq | NC_006351 |
| Uniprot ID | UPI00014F9A73 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| BPSS1495 (virA) promotor | ↓ | GCTAAGATCGATTGATGTCCGAAACAAAGACGGTTTAGTATTCGCCGA The sequence motif for the BsaN box as indicated in red |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| HTH_18 | PF12833.9 | 5e-17 | 141..220 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-221 | VTEIAKLDVSFGAMSHAVSIDKNKSALVIGIGQFKIASREGAWRFHDVPLFEAAKLLAFLESCGAPDGAGREYRAGAPDGAVIDAPGDLSIWKFDRWLIARVMGAPHGADPLFAFLRAQESYGLVRFLLRERANPQPIAALAARYGVSEPHFRRLCRQALGRGLKRELRQWRAAQAVLEVVESRDSMTEVAMSNGFASSSHFSREIKDLFGISPCRFRRRT |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9855 | - | - |
Protein sequence of regulator: 222 a.a. .
>REG0134 NC_006351:c2101230-2100565 [Burkholderia pseudomallei K96243]
VTEIAKLDVSFGAMSHAVSIDKNKSALVIGIGQFKIASREGAWRFHDVPLFEAAKLLAFLESCGAPDGAGREYRAGAPDG
AVIDAPGDLSIWKFDRWLIARVMGAPHGADPLFAFLRAQESYGLVRFLLRERANPQPIAALAARYGVSEPHFRRLCRQAL
GRGLKRELRQWRAAQAVLEVVESRDSMTEVAMSNGFASSSHFSREIKDLFGISPCRFRRRT*
VTEIAKLDVSFGAMSHAVSIDKNKSALVIGIGQFKIASREGAWRFHDVPLFEAAKLLAFLESCGAPDGAGREYRAGAPDG
AVIDAPGDLSIWKFDRWLIARVMGAPHGADPLFAFLRAQESYGLVRFLLRERANPQPIAALAARYGVSEPHFRRLCRQAL
GRGLKRELRQWRAAQAVLEVVESRDSMTEVAMSNGFASSSHFSREIKDLFGISPCRFRRRT*
Nucleotide sequence of regulator: 666 bp .
>REG0134 NC_006351:c2101230-2100565 [Burkholderia pseudomallei K96243]
GTGACGGAAATCGCGAAACTGGACGTGTCGTTCGGCGCGATGTCCCATGCCGTTTCGATCGACAAGAATAAATCGGCGCT
GGTTATCGGCATCGGTCAATTCAAAATCGCGTCGCGTGAAGGCGCATGGCGTTTTCATGACGTGCCGTTATTCGAGGCGG
CGAAATTGCTCGCTTTTCTGGAAAGCTGCGGCGCGCCGGACGGCGCCGGCCGCGAATATCGCGCGGGGGCGCCGGACGGC
GCGGTGATCGACGCGCCCGGCGATCTGAGCATCTGGAAATTCGACCGCTGGCTGATCGCCCGCGTGATGGGCGCGCCGCA
CGGCGCCGATCCGCTGTTCGCGTTCCTGCGCGCGCAGGAGAGCTACGGGCTCGTGCGCTTCTTGCTGCGCGAGCGTGCGA
ACCCGCAGCCGATCGCCGCGCTTGCCGCGCGCTACGGCGTGTCGGAGCCGCACTTTCGCCGGCTGTGCCGGCAGGCGCTC
GGCCGCGGCCTGAAGCGCGAGCTTCGGCAATGGCGCGCCGCGCAGGCGGTGCTCGAGGTGGTCGAGAGCCGCGACAGCAT
GACCGAGGTCGCGATGAGCAACGGCTTCGCGTCTTCGTCCCACTTCTCGCGGGAAATCAAGGACTTGTTCGGCATTTCGC
CGTGCCGGTTCCGTCGCCGGACATGA
GTGACGGAAATCGCGAAACTGGACGTGTCGTTCGGCGCGATGTCCCATGCCGTTTCGATCGACAAGAATAAATCGGCGCT
GGTTATCGGCATCGGTCAATTCAAAATCGCGTCGCGTGAAGGCGCATGGCGTTTTCATGACGTGCCGTTATTCGAGGCGG
CGAAATTGCTCGCTTTTCTGGAAAGCTGCGGCGCGCCGGACGGCGCCGGCCGCGAATATCGCGCGGGGGCGCCGGACGGC
GCGGTGATCGACGCGCCCGGCGATCTGAGCATCTGGAAATTCGACCGCTGGCTGATCGCCCGCGTGATGGGCGCGCCGCA
CGGCGCCGATCCGCTGTTCGCGTTCCTGCGCGCGCAGGAGAGCTACGGGCTCGTGCGCTTCTTGCTGCGCGAGCGTGCGA
ACCCGCAGCCGATCGCCGCGCTTGCCGCGCGCTACGGCGTGTCGGAGCCGCACTTTCGCCGGCTGTGCCGGCAGGCGCTC
GGCCGCGGCCTGAAGCGCGAGCTTCGGCAATGGCGCGCCGCGCAGGCGGTGCTCGAGGTGGTCGAGAGCCGCGACAGCAT
GACCGAGGTCGCGATGAGCAACGGCTTCGCGTCTTCGTCCCACTTCTCGCGGGAAATCAAGGACTTGTTCGGCATTTCGC
CGTGCCGGTTCCGTCGCCGGACATGA