Detailed information of regulator


Summary

Regulator ID REG0108 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00043 experimental (Type i1)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome
Sequence Protein sequence (544 a.a.); Nucleotide sequence (1632 bp)
Description VP1391 is predicted to be a member of the bacterial enhancer binding proteins (bEBPs) and contains a sigma-54 interaction domain and a helix-turn-helix domain. and required for expression of Hcp1 under marine-like conditions.; TfoY‐mediated activation of T6SS1 was dependent on the presence of both vp1391 and vp1407.
Reference

External database links

Locus tag (Gene) vp1391
Coordinates (Strand) 1491226..1492857 (-)
NCBI ID 28898165
RefSeq NC_004603
Uniprot ID Q87PV9_VIBPA
KEGG ID vpa:VP1391
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
T6SS1 (VgrG1) expression and secretion -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 5.3e-69 213..377
HTH_8 PF02954.21 5.6e-08 499..537

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-543 MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIPSTAHHNDKTIVSHPWEALQIDSASENDPFNFAIRNGEVVLINELYKYKGYDCESIYQTEQTLGLKSENLLAWPLIDSESKTIGLLVLLDLNVIDNEAALTEFCRMAASNIRQAVWLEQYGQVIKSLSADNQALVRENTQLKKRTQKAYQGPIAESEEMLNVLNRLDKVLSLPVDVLLRGETGAGKEVIAKYIHENSNRSEQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFEAANGGTLFLDEIGDMPMLLQAKLLRVLQERKVRPIGTSKEIEVDVRVIAATHCNLMQQIKDGGFRADLFYRLNVFPITLPPLRARKSDIIPLAEHFVQHTTNTLGLPQAPGLSANVRKQLLAYQYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDVPNIDMKAASPTPDQPAYERQAQADLEDASKSLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISAAG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8632 - -



  Protein sequence of regulator: 544 a.a.    .

>REG0108 NC_004603:c1492857-1491226 [Vibrio parahaemolyticus RIMD 2210633]
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIPSTAHHNDKTIVSHPWEALQI
DSASENDPFNFAIRNGEVVLINELYKYKGYDCESIYQTEQTLGLKSENLLAWPLIDSESKTIGLLVLLDLNVIDNEAALT
EFCRMAASNIRQAVWLEQYGQVIKSLSADNQALVRENTQLKKRTQKAYQGPIAESEEMLNVLNRLDKVLSLPVDVLLRGE
TGAGKEVIAKYIHENSNRSEQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFEAANGGTLFLDEIGDMPMLLQ
AKLLRVLQERKVRPIGTSKEIEVDVRVIAATHCNLMQQIKDGGFRADLFYRLNVFPITLPPLRARKSDIIPLAEHFVQHT
TNTLGLPQAPGLSANVRKQLLAYQYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDVPNIDMKAASPTPDQPAYER
QAQADLEDASKSLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISAAG*

  Nucleotide sequence of regulator: 1632 bp    .

>REG0108 NC_004603:c1492857-1491226 [Vibrio parahaemolyticus RIMD 2210633]
ATGCGTTCAGCTAACCATAGCGAACTTAGCAACGCCCTACTTGCGATCAGCCAATCTTTGGCTGATCGCAGCCAGCTTAC
CCAAACATTGGATGCCGTGTTGACCGCTGCGAGACAAATGACCTTAGCAAAGCACGGCATCATTTATGTTTTGGACCAGA
CAGGACAGGCGTTGATTCCAAGTACCGCTCACCATAACGATAAAACGATTGTTTCCCACCCTTGGGAGGCATTGCAGATT
GATTCCGCTAGTGAGAACGACCCATTTAACTTTGCCATTCGTAATGGTGAAGTGGTTTTGATTAACGAGTTATACAAATA
CAAAGGCTACGACTGCGAAAGCATTTATCAAACAGAGCAGACATTGGGACTAAAAAGCGAGAACTTACTGGCTTGGCCTT
TGATTGATAGCGAAAGCAAAACCATTGGGCTGTTAGTCTTACTCGATTTGAACGTCATCGATAACGAGGCAGCCTTGACT
GAGTTTTGTCGTATGGCTGCGAGTAATATTCGCCAAGCCGTTTGGTTAGAGCAATATGGGCAAGTGATTAAAAGCCTCAG
TGCCGATAATCAAGCCCTAGTGCGCGAGAACACTCAGCTCAAAAAACGTACTCAGAAAGCCTATCAAGGTCCTATTGCAG
AGAGCGAAGAAATGCTCAATGTCTTGAATCGTCTTGATAAGGTGCTATCTCTTCCTGTAGACGTTCTGTTGCGTGGTGAA
ACGGGCGCTGGTAAAGAGGTGATCGCGAAATACATTCACGAAAACTCCAACCGTTCAGAACAGCCTTTGATCGTACAAAA
CTGTGCGGCAATTCCCGAACAGTTGTTAGAGTCTGAACTGTTTGGGCATAAGAAAGGTTCATTCACGGGCGCGGACAAAG
ACAAAGTCGGTTTGTTTGAAGCAGCCAACGGCGGCACGCTTTTCCTAGATGAAATTGGCGATATGCCAATGCTACTACAA
GCGAAGTTACTGCGTGTACTGCAAGAGCGTAAAGTTCGCCCTATCGGTACCAGCAAAGAGATTGAAGTCGACGTTCGCGT
TATCGCTGCAACCCACTGTAATCTTATGCAGCAAATCAAAGATGGCGGATTCCGTGCCGATTTGTTCTATCGTTTGAATG
TGTTCCCTATTACATTACCACCATTGAGAGCTCGCAAGTCAGACATTATTCCCCTTGCCGAACATTTCGTGCAACACACA
ACCAACACGCTAGGTTTGCCGCAAGCGCCAGGGTTAAGTGCCAACGTACGTAAACAGCTCCTTGCCTACCAATACCCAGG
GAACGTGCGTGAGCTTAAGAACATCATTGAGCGCTCGGTGCTACTGTCCGATTTTGAAACCATTACTCACATTGAGTTTG
GCGAACAAATCCCAGAAGATGTGCCAAATATCGACATGAAAGCAGCCTCACCTACCCCAGACCAACCTGCTTACGAGCGG
CAGGCACAAGCGGATTTAGAAGACGCCTCAAAGAGCCTTAAGGATGTTGTCAGCCAATACGAGCGTACAGTGATCATCGA
TTGCTTAAATGCGTGTAATTGGCACACCAAGAAGGCCGCTGAGCAATTGGCGCTGCCAATGAGCACTCTCAACCATAAGA
TGAAGAAATACGACATTTCTGCGGCAGGTTGA