Detailed information of regulator


Summary

Regulator ID REG0099 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00041 insolico (Type i1); T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (506 a.a.); Nucleotide sequence (1518 bp)
Description GUS reporter assay to check the promoter activities of tssM2 and tssB2/C2 operons in the presence or absence of sigma-54-dependent Fis family transcriptional Sfa2 and similar to the hcp2 operon, the promoter activities of these two operons were decreased in Δsfa2 as compared with the wild type.
Reference
External database links

Locus tag (Gene) PSPTO_RS28060 (Sfa)
Coordinates (Strand) 6165047..6166564 (-)
NCBI ID 499418021
RefSeq NC_004578
Uniprot ID Q87U83_PSESM
KEGG ID pst:PSPTO_5424
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 1.7e-69 196..363
HTH_8 PF02954.21 1.6e-10 456..495
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-505 MFTQMPQPLAYAQALLAQFASLARAVNGAQLLGDFVRSLAELSGCELSQLYLLDATHKCLKMRAECLDGTLQPREAASLPVDYNGEQLLQFALCQNRVVCLGELSGSLHETSFLPTQSTPWQSLLCVPVVNHQQAVEGLLLCASRRHVDLQGFADSLGQFGSFALGQLNLLQRLHKPADEVRPAGITMPSVSGYGLIGKSASMRQTYTMISKVLHSPYTVLLRGETGTGKEVVARAIHDFGPRRSQPFIVQNCAAFPEHLLESELFGYRKGAFTGADSDRTGLFEAANGGTLLLDEIGDIPLPLQAKLLRVLQEGEIRPLGCNDTRKIDVRILAATHRDLSAMVSEGKFREDLYYRLAQFPIELPALRNREGDILDLARHFVDKTCAFLQRSPLRWSDAALDLLSGYTFPGNVRELKGLVERAVLLCEGSELLVEHFSLRPEVVPEGSSLNLRERLEQVERSLLLDCLRKNDGNQTLSARELGLPRRTLLYRLGRLNINLGDLNG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.6156 pos: 25-26. ARA-VN. MFTQMPQPLAYAQALLAQFASLARA


  Protein sequence of regulator: 506 a.a.    .

>REG0099 NC_004578:c6166564-6165047 [Pseudomonas syringae pv. tomato str. DC3000]
MFTQMPQPLAYAQALLAQFASLARAVNGAQLLGDFVRSLAELSGCELSQLYLLDATHKCLKMRAECLDGTLQPREAASLP
VDYNGEQLLQFALCQNRVVCLGELSGSLHETSFLPTQSTPWQSLLCVPVVNHQQAVEGLLLCASRRHVDLQGFADSLGQF
GSFALGQLNLLQRLHKPADEVRPAGITMPSVSGYGLIGKSASMRQTYTMISKVLHSPYTVLLRGETGTGKEVVARAIHDF
GPRRSQPFIVQNCAAFPEHLLESELFGYRKGAFTGADSDRTGLFEAANGGTLLLDEIGDIPLPLQAKLLRVLQEGEIRPL
GCNDTRKIDVRILAATHRDLSAMVSEGKFREDLYYRLAQFPIELPALRNREGDILDLARHFVDKTCAFLQRSPLRWSDAA
LDLLSGYTFPGNVRELKGLVERAVLLCEGSELLVEHFSLRPEVVPEGSSLNLRERLEQVERSLLLDCLRKNDGNQTLSAR
ELGLPRRTLLYRLGRLNINLGDLNG*

  Nucleotide sequence of regulator: 1518 bp    .

>REG0099 NC_004578:c6166564-6165047 [Pseudomonas syringae pv. tomato str. DC3000]
ATGTTCACTCAGATGCCGCAGCCTCTGGCATATGCCCAAGCCTTGTTGGCGCAATTCGCCAGCTTGGCGCGGGCGGTGAA
TGGTGCCCAATTGCTGGGTGATTTTGTACGTAGCCTCGCTGAGCTCAGCGGCTGCGAGCTGTCGCAGCTTTATCTGCTGG
ACGCCACTCATAAGTGTTTGAAGATGAGAGCCGAGTGCCTGGACGGCACGCTGCAACCGCGGGAAGCGGCAAGCCTGCCG
GTGGATTACAACGGCGAACAGCTGTTGCAATTTGCCCTTTGTCAGAATCGTGTGGTTTGCCTTGGCGAACTGAGCGGCAG
CCTGCACGAAACCAGTTTTCTGCCGACGCAAAGTACGCCGTGGCAGTCGCTGCTTTGTGTCCCGGTCGTCAACCATCAGC
AAGCCGTCGAAGGCTTGTTGTTGTGCGCAAGTCGCCGACATGTCGATCTGCAGGGTTTTGCTGATTCTCTCGGTCAGTTC
GGCTCGTTCGCACTGGGTCAACTCAACCTGCTGCAACGTTTGCATAAGCCTGCGGATGAGGTACGACCGGCAGGGATCAC
TATGCCAAGCGTCAGCGGATACGGTTTGATCGGAAAAAGCGCAAGCATGCGTCAGACCTACACGATGATCAGCAAGGTGC
TGCACAGCCCGTACACTGTGTTGCTGCGGGGAGAGACAGGCACCGGCAAAGAAGTCGTTGCCCGGGCGATTCACGACTTC
GGTCCGCGTCGCTCTCAGCCTTTCATTGTTCAGAACTGTGCGGCATTTCCAGAGCACCTGCTTGAAAGCGAGCTTTTCGG
CTATCGCAAAGGAGCCTTTACAGGTGCCGACAGTGATCGTACCGGGCTGTTCGAGGCTGCCAATGGCGGCACACTGTTGC
TCGACGAAATCGGCGACATTCCCCTGCCGCTGCAAGCCAAGCTGCTGCGCGTGCTGCAAGAGGGCGAGATTCGCCCATTG
GGTTGCAACGATACGCGCAAGATCGACGTGCGTATTCTTGCAGCAACGCATCGTGACCTGTCGGCGATGGTCAGCGAAGG
CAAATTCCGCGAGGACTTGTATTACCGTCTTGCGCAATTTCCCATTGAGCTGCCTGCCCTGCGCAATCGAGAGGGTGACA
TTCTCGACCTGGCGCGGCACTTCGTCGACAAGACGTGTGCGTTCTTGCAACGTAGCCCTTTGCGCTGGTCGGACGCAGCT
CTTGATCTCCTGTCCGGTTATACGTTTCCCGGCAATGTGCGCGAACTCAAAGGCCTGGTCGAGCGTGCTGTCCTGCTCTG
CGAGGGCAGCGAGTTGCTTGTGGAGCATTTCTCTCTGCGCCCGGAAGTTGTGCCTGAAGGCAGCAGCCTCAATCTGCGCG
AGCGGCTGGAGCAGGTGGAGCGCAGCCTGTTGCTTGATTGCCTGCGCAAGAACGACGGCAACCAGACTCTTTCCGCGCGT
GAACTCGGCCTGCCGAGGCGCACGCTGCTTTACCGTTTGGGACGCCTGAATATCAACCTGGGTGACTTAAATGGTTAG