Detailed information of regulator
Summary
| Regulator ID | REG0099  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00041  (Type i1); T6SS00042 (Type i1) |
| Strain | Pseudomonas syringae pv. tomato str. DC3000 |
| Replicon | chromosome |
| Sequence | Protein sequence (506 a.a.); Nucleotide sequence (1518 bp) |
| Description | GUS reporter assay to check the promoter activities of tssM2 and tssB2/C2 operons in the presence or absence of sigma-54-dependent Fis family transcriptional Sfa2 and similar to the hcp2 operon, the promoter activities of these two operons were decreased in Δsfa2 as compared with the wild type. |
| Reference |
External database links
| Locus tag (Gene) | PSPTO_RS28060 (Sfa) |
| Coordinates (Strand) | 6165047..6166564 (-) |
| NCBI ID | 499418021 |
| RefSeq | NC_004578 |
| Uniprot ID | Q87U83_PSESM |
| KEGG ID | pst:PSPTO_5424 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
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Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS transcripts | ↓ | - |
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Pfam domain hit(s) of regulator
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Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Sigma54_activat | PF00158.28 | 1.7e-69 | 196..363 |
| HTH_8 | PF02954.21 | 1.6e-10 | 456..495 |
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Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
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Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-505 | MFTQMPQPLAYAQALLAQFASLARAVNGAQLLGDFVRSLAELSGCELSQLYLLDATHKCLKMRAECLDGTLQPREAASLPVDYNGEQLLQFALCQNRVVCLGELSGSLHETSFLPTQSTPWQSLLCVPVVNHQQAVEGLLLCASRRHVDLQGFADSLGQFGSFALGQLNLLQRLHKPADEVRPAGITMPSVSGYGLIGKSASMRQTYTMISKVLHSPYTVLLRGETGTGKEVVARAIHDFGPRRSQPFIVQNCAAFPEHLLESELFGYRKGAFTGADSDRTGLFEAANGGTLLLDEIGDIPLPLQAKLLRVLQEGEIRPLGCNDTRKIDVRILAATHRDLSAMVSEGKFREDLYYRLAQFPIELPALRNREGDILDLARHFVDKTCAFLQRSPLRWSDAALDLLSGYTFPGNVRELKGLVERAVLLCEGSELLVEHFSLRPEVVPEGSSLNLRERLEQVERSLLLDCLRKNDGNQTLSARELGLPRRTLLYRLGRLNINLGDLNG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.6156 | pos: 25-26. ARA-VN. | MFTQMPQPLAYAQALLAQFASLARA |
Protein sequence of regulator: 506 a.a. .
>REG0099 NC_004578:c6166564-6165047 [Pseudomonas syringae pv. tomato str. DC3000]
MFTQMPQPLAYAQALLAQFASLARAVNGAQLLGDFVRSLAELSGCELSQLYLLDATHKCLKMRAECLDGTLQPREAASLP
VDYNGEQLLQFALCQNRVVCLGELSGSLHETSFLPTQSTPWQSLLCVPVVNHQQAVEGLLLCASRRHVDLQGFADSLGQF
GSFALGQLNLLQRLHKPADEVRPAGITMPSVSGYGLIGKSASMRQTYTMISKVLHSPYTVLLRGETGTGKEVVARAIHDF
GPRRSQPFIVQNCAAFPEHLLESELFGYRKGAFTGADSDRTGLFEAANGGTLLLDEIGDIPLPLQAKLLRVLQEGEIRPL
GCNDTRKIDVRILAATHRDLSAMVSEGKFREDLYYRLAQFPIELPALRNREGDILDLARHFVDKTCAFLQRSPLRWSDAA
LDLLSGYTFPGNVRELKGLVERAVLLCEGSELLVEHFSLRPEVVPEGSSLNLRERLEQVERSLLLDCLRKNDGNQTLSAR
ELGLPRRTLLYRLGRLNINLGDLNG*
MFTQMPQPLAYAQALLAQFASLARAVNGAQLLGDFVRSLAELSGCELSQLYLLDATHKCLKMRAECLDGTLQPREAASLP
VDYNGEQLLQFALCQNRVVCLGELSGSLHETSFLPTQSTPWQSLLCVPVVNHQQAVEGLLLCASRRHVDLQGFADSLGQF
GSFALGQLNLLQRLHKPADEVRPAGITMPSVSGYGLIGKSASMRQTYTMISKVLHSPYTVLLRGETGTGKEVVARAIHDF
GPRRSQPFIVQNCAAFPEHLLESELFGYRKGAFTGADSDRTGLFEAANGGTLLLDEIGDIPLPLQAKLLRVLQEGEIRPL
GCNDTRKIDVRILAATHRDLSAMVSEGKFREDLYYRLAQFPIELPALRNREGDILDLARHFVDKTCAFLQRSPLRWSDAA
LDLLSGYTFPGNVRELKGLVERAVLLCEGSELLVEHFSLRPEVVPEGSSLNLRERLEQVERSLLLDCLRKNDGNQTLSAR
ELGLPRRTLLYRLGRLNINLGDLNG*
Nucleotide sequence of regulator: 1518 bp .
>REG0099 NC_004578:c6166564-6165047 [Pseudomonas syringae pv. tomato str. DC3000]
ATGTTCACTCAGATGCCGCAGCCTCTGGCATATGCCCAAGCCTTGTTGGCGCAATTCGCCAGCTTGGCGCGGGCGGTGAA
TGGTGCCCAATTGCTGGGTGATTTTGTACGTAGCCTCGCTGAGCTCAGCGGCTGCGAGCTGTCGCAGCTTTATCTGCTGG
ACGCCACTCATAAGTGTTTGAAGATGAGAGCCGAGTGCCTGGACGGCACGCTGCAACCGCGGGAAGCGGCAAGCCTGCCG
GTGGATTACAACGGCGAACAGCTGTTGCAATTTGCCCTTTGTCAGAATCGTGTGGTTTGCCTTGGCGAACTGAGCGGCAG
CCTGCACGAAACCAGTTTTCTGCCGACGCAAAGTACGCCGTGGCAGTCGCTGCTTTGTGTCCCGGTCGTCAACCATCAGC
AAGCCGTCGAAGGCTTGTTGTTGTGCGCAAGTCGCCGACATGTCGATCTGCAGGGTTTTGCTGATTCTCTCGGTCAGTTC
GGCTCGTTCGCACTGGGTCAACTCAACCTGCTGCAACGTTTGCATAAGCCTGCGGATGAGGTACGACCGGCAGGGATCAC
TATGCCAAGCGTCAGCGGATACGGTTTGATCGGAAAAAGCGCAAGCATGCGTCAGACCTACACGATGATCAGCAAGGTGC
TGCACAGCCCGTACACTGTGTTGCTGCGGGGAGAGACAGGCACCGGCAAAGAAGTCGTTGCCCGGGCGATTCACGACTTC
GGTCCGCGTCGCTCTCAGCCTTTCATTGTTCAGAACTGTGCGGCATTTCCAGAGCACCTGCTTGAAAGCGAGCTTTTCGG
CTATCGCAAAGGAGCCTTTACAGGTGCCGACAGTGATCGTACCGGGCTGTTCGAGGCTGCCAATGGCGGCACACTGTTGC
TCGACGAAATCGGCGACATTCCCCTGCCGCTGCAAGCCAAGCTGCTGCGCGTGCTGCAAGAGGGCGAGATTCGCCCATTG
GGTTGCAACGATACGCGCAAGATCGACGTGCGTATTCTTGCAGCAACGCATCGTGACCTGTCGGCGATGGTCAGCGAAGG
CAAATTCCGCGAGGACTTGTATTACCGTCTTGCGCAATTTCCCATTGAGCTGCCTGCCCTGCGCAATCGAGAGGGTGACA
TTCTCGACCTGGCGCGGCACTTCGTCGACAAGACGTGTGCGTTCTTGCAACGTAGCCCTTTGCGCTGGTCGGACGCAGCT
CTTGATCTCCTGTCCGGTTATACGTTTCCCGGCAATGTGCGCGAACTCAAAGGCCTGGTCGAGCGTGCTGTCCTGCTCTG
CGAGGGCAGCGAGTTGCTTGTGGAGCATTTCTCTCTGCGCCCGGAAGTTGTGCCTGAAGGCAGCAGCCTCAATCTGCGCG
AGCGGCTGGAGCAGGTGGAGCGCAGCCTGTTGCTTGATTGCCTGCGCAAGAACGACGGCAACCAGACTCTTTCCGCGCGT
GAACTCGGCCTGCCGAGGCGCACGCTGCTTTACCGTTTGGGACGCCTGAATATCAACCTGGGTGACTTAAATGGTTAG
ATGTTCACTCAGATGCCGCAGCCTCTGGCATATGCCCAAGCCTTGTTGGCGCAATTCGCCAGCTTGGCGCGGGCGGTGAA
TGGTGCCCAATTGCTGGGTGATTTTGTACGTAGCCTCGCTGAGCTCAGCGGCTGCGAGCTGTCGCAGCTTTATCTGCTGG
ACGCCACTCATAAGTGTTTGAAGATGAGAGCCGAGTGCCTGGACGGCACGCTGCAACCGCGGGAAGCGGCAAGCCTGCCG
GTGGATTACAACGGCGAACAGCTGTTGCAATTTGCCCTTTGTCAGAATCGTGTGGTTTGCCTTGGCGAACTGAGCGGCAG
CCTGCACGAAACCAGTTTTCTGCCGACGCAAAGTACGCCGTGGCAGTCGCTGCTTTGTGTCCCGGTCGTCAACCATCAGC
AAGCCGTCGAAGGCTTGTTGTTGTGCGCAAGTCGCCGACATGTCGATCTGCAGGGTTTTGCTGATTCTCTCGGTCAGTTC
GGCTCGTTCGCACTGGGTCAACTCAACCTGCTGCAACGTTTGCATAAGCCTGCGGATGAGGTACGACCGGCAGGGATCAC
TATGCCAAGCGTCAGCGGATACGGTTTGATCGGAAAAAGCGCAAGCATGCGTCAGACCTACACGATGATCAGCAAGGTGC
TGCACAGCCCGTACACTGTGTTGCTGCGGGGAGAGACAGGCACCGGCAAAGAAGTCGTTGCCCGGGCGATTCACGACTTC
GGTCCGCGTCGCTCTCAGCCTTTCATTGTTCAGAACTGTGCGGCATTTCCAGAGCACCTGCTTGAAAGCGAGCTTTTCGG
CTATCGCAAAGGAGCCTTTACAGGTGCCGACAGTGATCGTACCGGGCTGTTCGAGGCTGCCAATGGCGGCACACTGTTGC
TCGACGAAATCGGCGACATTCCCCTGCCGCTGCAAGCCAAGCTGCTGCGCGTGCTGCAAGAGGGCGAGATTCGCCCATTG
GGTTGCAACGATACGCGCAAGATCGACGTGCGTATTCTTGCAGCAACGCATCGTGACCTGTCGGCGATGGTCAGCGAAGG
CAAATTCCGCGAGGACTTGTATTACCGTCTTGCGCAATTTCCCATTGAGCTGCCTGCCCTGCGCAATCGAGAGGGTGACA
TTCTCGACCTGGCGCGGCACTTCGTCGACAAGACGTGTGCGTTCTTGCAACGTAGCCCTTTGCGCTGGTCGGACGCAGCT
CTTGATCTCCTGTCCGGTTATACGTTTCCCGGCAATGTGCGCGAACTCAAAGGCCTGGTCGAGCGTGCTGTCCTGCTCTG
CGAGGGCAGCGAGTTGCTTGTGGAGCATTTCTCTCTGCGCCCGGAAGTTGTGCCTGAAGGCAGCAGCCTCAATCTGCGCG
AGCGGCTGGAGCAGGTGGAGCGCAGCCTGTTGCTTGATTGCCTGCGCAAGAACGACGGCAACCAGACTCTTTCCGCGCGT
GAACTCGGCCTGCCGAGGCGCACGCTGCTTTACCGTTTGGGACGCCTGAATATCAACCTGGGTGACTTAAATGGTTAG