Detailed information of regulator


Summary

Regulator ID REG0097 experimental
Regulator type protein
Regulatory pathway (from literatures) (p)ppGpp regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Purine metabolism (map00230)
Related T6SS T6SS00041 insolico (Type i1); T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (702 a.a.); Nucleotide sequence (2106 bp)
Description 28 putative T6SS-associated genes of PstDC3000 and 25 T6SS-related genes of PssB728a were down-regulated in the (p)ppGpp0PstDC3000 and (p)ppGpp0PssB728a mutants, respectively.
Reference
External database links

Locus tag (Gene) PSPTO_RS00410 (spoT)
Coordinates (Strand) 94805..96910 (-)
NCBI ID 492171753
RefSeq NC_004578
Uniprot ID Q88BE4_PSESM, A0A0P9JHG4_9PSED, A0A0P9K127_9PSED, A0A2S3UZF1_PSESX, A0A0N0G206_PSEYM, F3INA6_PSEAV, A0A2K4VLK4_PSESX, A0A0P9HXH2_9PSED
KEGG ID pst:PSPTO_0073
KEGG pathway (determined by BlastKOALA) map01100; map00230
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HD_4 PF13328.8 1.2e-50 26..175
RelA_SpoT PF04607.19 4.3e-36 235..344
TGS PF02824.23 6.9e-18 389..447
ACT_4 PF13291.8 2.4e-15 622..698
DUF5913 PF19296.1 1e-08 460..543
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-701 MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRRSESINLGERLLNKVLNGFECSLDKVTPERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGEQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9928 - -


  Protein sequence of regulator: 702 a.a.    .

>REG0097 NC_004578:c96910-94805 [Pseudomonas syringae pv. tomato str. DC3000]
MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGI
AKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
ETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHL
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEI
QIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIME
LPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESINLGERLLNKVLNGFECSLDKVTPERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGEQLPSPEGPLAIR
GTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIA
LLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA*

  Nucleotide sequence of regulator: 2106 bp    .

>REG0097 NC_004578:c96910-94805 [Pseudomonas syringae pv. tomato str. DC3000]
TTGCCGAGCATAGACGCCCTCGCCGACAGACTGTCGGCCTACCTCGGTAAGGACCAGGTCAATCTGGTCCGCCGAGCGTA
TTTCTACGCCGAACAAGCCCACGATGGCCAGCGCCGTCGCAGCGGTGAAGCCTACGTCACCCATCCTCTTGCGGTAGCCA
ATATCCTTGCTGACATGCACATGGACCATCAGAGCCTGATGGCTGCGATGCTGCATGACGTCATCGAAGACACCGGTATT
GCCAAGGAAGCGCTCAGCGCGCAGTTTGGCGAAACCGTCGCGGAACTGGTCGACGGGGTCAGCAAACTGACCCAGATGAA
CTTCGAGACCAAAGCCGAAGCTCAGGCCGAAAACTTCCAGAAAATGGCCATGGCGATGGCGCGGGACATCCGCGTGATTC
TGGTCAAGCTCGCCGACCGCCTGCACAACATGCGCACACTGGAAGTCCTGTCCGGTGAAAAGCGCCGCCGCATCGCCAAG
GAAACCCTCGAAATCTATGCCCCCATCGCCAATCGGCTGGGCATGCACAGTGTGCGCATCGAATTTGAAGACCTGGGTTT
CAAGGCCATGTACCCGATGCGCTCCTCGCGAATCAACCAGGCGGTCAAGCGCGCCAGGGGCAATCGCAAGGAGCTGGTCA
ACAAGATCGAAGAGTCCCTGAGCCATTGCCTTGCGGTGGATGGCATCGAAGGCGACGTCAGCGGGCGGCAGAAACACCTC
TACGGCATCTACAAGAAGATGCGCGGCAAGCGCCGGGCCTTCAACGAGATCATGGACGTTTACGCGTTCCGCATCATCGT
CGACAAGGTCGATACCTGTTATCGCGTGCTGGGCGCTGTACATAATCTGTACAAGCCGCTGCCCGGCCGATTCAAGGATT
ACATCGCGATCCCCAAGGCCAACGGCTACCAGTCGTTGCATACCACGCTGTTCGGCATGCACGGCGTACCGATCGAAATC
CAGATTCGCACACGCGAAATGGAAGAGATGGCCAATAACGGCATCGCTGCGCACTGGCTGTACAAATCCAGTGATGACGA
GCAGCCCAAGGGCACTCACGCCCGCGCCCGGCAGTGGGTCAAGGGCGTGCTGGAAATGCAGCAGCGCGCGGGCAACTCCC
TGGAGTTCATCGAGAGCGTCAAGATCGACCTGTTCCCGGACGAGGTCTACGTTTTCACGCCCAAGGGCCGGATCATGGAG
CTGCCCAAAGGCTCCACGGCAGTGGATTTTGCCTATGCAGTGCATACCGATGTCGGCAACAGCTGCATTGCCTGTCGCAT
CAACCGTCGACTGGCGCCATTGTCCGAGCCGCTGCAGAGCGGCTCCACGGTCGAGATTGTCAGCGCCCCCGGCGCACGTC
CGAACCCGGCATGGCTGAACTTCGTCGTCACCGGCAAGGCACGTACCCATATTCGCCACGCGCTCAAGCTGCAACGCCGC
TCCGAGTCGATCAACCTTGGCGAGCGCCTGCTCAACAAGGTGCTTAACGGCTTTGAATGCAGCCTGGACAAGGTCACGCC
AGAGCGCATTCAACTGGTGCTGAATGAATATCGGGTCGAGGTGCTGGAAGACCTGCTCGAAGACATCGGCCTGGGCAATC
GCATGGCCTATGTCGTCGCCCGCCGCTTGCTGGGCGAAGGCGAGCAACTGCCAAGCCCGGAAGGCCCGCTGGCGATTCGC
GGCACCGAAGGTCTGGTACTCAGCTACGCCAAATGCTGTACGCCGATCCCGGGCGACCCGATTGTCGGCCATTTGTCTGC
GGGCAAGGGCATGGTCGTGCACCTGGATAACTGCCGTAATATCAGCGAAATCCGCCACAACCCGGAAAAATGCATCCAGC
TTTCCTGGGCCAAGGACGTCACCGGCGAATTCAATGTCGAGCTGCGTGTCGAGCTAGAGCACCAGCGCGGTCTGATCGCC
TTGCTGGCCAGCAGCGTCAACGCAGCCGATGGCAATATCGAAAAAATCAGTATGGACGAACGCGATGGTCGCATCAGCGT
GGTCCAACTGGTGGTCAGTGTGCACGACCGCGTGCACCTGGCCCGCGTGATCAAGAAACTGCGCGCCTTGACCGGTGTCA
CACGCATCACCCGGATGCGCGCGTAG