Detailed information of regulator


Summary

Regulator ID REG0096 experimental
Regulator type protein
Regulatory pathway (from literatures) (p)ppGpp regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Purine metabolism (map00230)
Related T6SS T6SS00041 insolico (Type i1); T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (748 a.a.); Nucleotide sequence (2244 bp)
Description 28 putative T6SS-associated genes of PstDC3000 and 25 T6SS-related genes of PssB728a were down-regulated in the (p)ppGpp0PstDC3000 and (p)ppGpp0PssB728a mutants, respectively.
Reference
External database links

Locus tag (Gene) PSPTO_RS08845 (relA)
Coordinates (Strand) 1863879..1866122 (+)
NCBI ID 494457621
RefSeq NC_004578
Uniprot ID A0A2S3UVS0_PSESX, A0A099SIA5_PSEUB, A0A2K4VQZ7_PSESX
KEGG ID -
KEGG pathway (determined by BlastKOALA) map01100; map00230
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HD_4 PF13328.8 1.4e-44 40..202
RelA_SpoT PF04607.19 9.1e-38 262..370
TGS PF02824.23 4.1e-21 416..475
ACT_4 PF13291.8 8.2e-17 667..743
DUF5913 PF19296.1 3.6e-08 494..637
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-747 MVQVRANQPINTDGSINLDAWLDHIVSVDLVLDREVMKQACEFARQAEQNDKAHKNHWAEGTSSFQTGLEIAEILADLKLDQDSLVAAVMYRGVREGVIPLPDVEQRFGATVAKLIDGVLRMAAISASLSPRQSLVLGSQAQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKNADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFISDVMSQLDNELQATGVTADISGRAKHIYSIWRKMQRKGLAFSQIYDVRALRVLVPEMRDCYTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAELGVCAHWRYKGTDVKSSSNHYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYVFTPDGHAIDLPKNATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGTPSRDWLNSNLGYITTSRARAKIVHWFKLQARDQNVAAGKTLIERELARLALPQVDFDKLADKANHKTADDMFAALGAGDLRLAQLVNLAQQQVEPDRVNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLGGREPERIIQVSWGPVPVLTYPVDIIIRAYDRSGLLRDVTQVLLNERINVLAVNTRSNKEDNTALMSLTIEIPGLDALGRLLGRISQLPNIIETRRNRTP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9961 - -


  Protein sequence of regulator: 748 a.a.    .

>REG0096 NC_004578:1863879-1866122 [Pseudomonas syringae pv. tomato str. DC3000]
MVQVRANQPINTDGSINLDAWLDHIVSVDLVLDREVMKQACEFARQAEQNDKAHKNHWAEGTSSFQTGLEIAEILADLKL
DQDSLVAAVMYRGVREGVIPLPDVEQRFGATVAKLIDGVLRMAAISASLSPRQSLVLGSQAQVENLRKMLVAMVDDVRVA
LIKLAERTCAIRAVKNADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
SDVMSQLDNELQATGVTADISGRAKHIYSIWRKMQRKGLAFSQIYDVRALRVLVPEMRDCYTALGIVHTLWRHIPKEFDD
YIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAELGVCAHWRYKGTDVKSSSNHYEEKISWLRQVLEWHEELGDI
GGLAEQLRVDIEPDRVYVFTPDGHAIDLPKNATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNSNLGYITTSRARAKIVHWFKLQARDQNVAAGKTLIERELARLALPQVDFDKLADKANHKTADDMFAALGAGDL
RLAQLVNLAQQQVEPDRVNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQD
CASVLQLGGREPERIIQVSWGPVPVLTYPVDIIIRAYDRSGLLRDVTQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP*

  Nucleotide sequence of regulator: 2244 bp    .

>REG0096 NC_004578:1863879-1866122 [Pseudomonas syringae pv. tomato str. DC3000]
ATGGTACAGGTGAGAGCGAACCAGCCGATAAACACAGACGGCAGTATCAATCTCGACGCATGGCTCGATCATATTGTCAG
CGTCGACCTGGTCCTGGACCGGGAAGTCATGAAGCAGGCCTGTGAATTTGCCCGGCAAGCCGAGCAGAATGACAAGGCTC
ACAAGAATCATTGGGCAGAAGGCACCTCCAGTTTCCAGACCGGTCTGGAAATCGCGGAGATCCTCGCTGACCTCAAGCTG
GATCAGGATTCGCTGGTCGCGGCGGTCATGTACCGTGGCGTGCGCGAAGGCGTGATTCCGTTGCCGGACGTCGAGCAGCG
CTTTGGTGCCACGGTCGCCAAGCTGATCGACGGCGTGCTGCGCATGGCGGCGATCAGCGCCAGCCTCAGCCCGCGACAAT
CGCTGGTGCTGGGCAGCCAGGCGCAGGTCGAAAACCTGCGCAAGATGCTTGTGGCGATGGTCGATGACGTGCGCGTTGCA
CTCATCAAGCTGGCCGAGCGCACCTGTGCGATCCGTGCGGTGAAGAACGCCGACGACGAAAAGCGCAACCGCGTGGCCCG
CGAGGTGTTCGACATCTATGCGCCGCTGGCCCACCGTCTGGGCATCGGCCATATCAAATGGGAGCTGGAAGACCTTTCTT
TCCGCTACCTGGAGCCCGACCAATACAAGCAGATCGCCAAGCTGCTGCACGAGCGCAGGCTCGATCGCGAGCGCTTCATC
AGCGACGTCATGTCCCAGCTCGACAATGAGCTGCAAGCCACCGGCGTGACGGCGGACATCAGTGGCCGTGCCAAGCACAT
CTATTCCATCTGGCGCAAAATGCAGCGCAAGGGCTTGGCGTTCAGCCAGATCTACGACGTGCGTGCATTGCGCGTGCTGG
TCCCGGAAATGCGTGATTGCTACACCGCGCTGGGTATCGTGCACACGCTGTGGCGGCATATTCCCAAGGAGTTCGACGAC
TACATCGCCAACCCCAAGGAAAACGGCTATCGCTCGCTGCACACCGCCGTCATCGGGCCGGAAGGCAAGGTGCTGGAAGT
GCAGATCCGCACCCACGCCATGCACGAAGAGGCCGAGCTCGGCGTTTGCGCGCACTGGCGCTATAAAGGCACCGACGTCA
AATCCAGCTCCAATCATTACGAAGAAAAGATTTCCTGGCTGCGTCAGGTCCTTGAATGGCATGAAGAACTGGGTGACATC
GGTGGTCTGGCCGAGCAGTTGCGGGTCGATATCGAGCCGGACCGTGTCTACGTCTTCACCCCCGACGGCCACGCCATCGA
CCTGCCCAAGAACGCGACGCCGCTGGACTTCGCCTATCGGGTGCACACCGAGATCGGCCATAACTGCCGTGGTGCGAAGA
TCAACGGCCGCATCGTACCGCTCAACTACAGCCTGCAAACCGGCGAGCAGGTCGAGATCATCACCAGCAAGCACGGCACG
CCGAGCCGCGACTGGCTGAACTCCAACCTGGGCTACATCACCACGTCCCGGGCGCGGGCGAAGATTGTTCACTGGTTCAA
ATTGCAGGCCCGTGACCAGAACGTCGCGGCCGGTAAAACCCTGATCGAACGTGAGCTGGCGCGTCTGGCGTTGCCGCAGG
TCGATTTCGACAAGCTGGCAGACAAAGCCAATCACAAGACCGCCGACGATATGTTTGCCGCACTGGGCGCGGGGGATCTG
CGCCTGGCGCAACTGGTCAACCTGGCGCAACAGCAGGTCGAGCCGGATCGGGTTAATGAACAACTGGAACTGATCCCGCG
CAAGGCCACCGGCTACAAGCCTGGCAAGCGCGGCGATATCCAGATTCAGGGCGTCGGCAACCTGATGACGCAAATGGCCG
GCTGCTGCCAGCCATTACCGGGCGACGCGATTGTCGGGTACATCACGCAGGGGCGTGGCGTCAGCATTCACCGTCAGGAT
TGCGCCTCGGTGCTGCAACTGGGCGGTCGCGAACCCGAGCGGATCATCCAGGTCAGTTGGGGCCCGGTGCCGGTGCTCAC
CTACCCGGTGGACATCATCATCCGTGCCTACGACCGTTCCGGCCTGCTGCGTGACGTCACCCAGGTGCTGCTCAACGAGC
GCATCAACGTACTGGCGGTCAACACCCGCTCGAACAAGGAAGACAACACCGCGCTGATGTCGCTGACCATCGAGATTCCG
GGGCTGGACGCGCTGGGCCGGTTGCTGGGGCGGATATCCCAGTTGCCGAACATTATTGAAACACGCCGAAACAGGACGCC
TTGA