Detailed information of regulator


Summary

Regulator ID REG0054 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (796 a.a.); Nucleotide sequence (2388 bp)
Description The gene encoding Hcp1 resides in the IAHP-related virulence locus regulated by RetS and LadS.
Reference
External database links

Locus tag (Gene) PA3974 (LadS)
Coordinates (Strand) 4453289..4455676 (-)
NCBI ID 15599169
RefSeq NC_002516
Uniprot ID Q9HX42_PSEAE
KEGG ID pae:PA3974
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
hcp1 (PA0085) expression and secretion ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
7TMR-DISM_7TM PF07695.13 5.9e-56 184..386
7TMR-DISMED2 PF07696.13 5.8e-37 32..171
Response_reg PF00072.26 5.1e-27 670..780
HATPase_c PF02518.28 6.1e-23 530..644
HisKA PF00512.27 2.4e-14 419..482
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-182 MRHWLILFLLALPCLAGAVSFNEQVERLPLGQSIDVFEDVRGSADINDITSRAIDSSFRRHDKDVLNAGYSRSVFWLRLDLDYRPVASSDPRTWLLELAYPPLDKLDLYLPDGQGGYRLAQRTGDTLPFASRPIRQNNYLFELGLEPNKPQRVYLRLESQGSIQAPLTLWSPKAYLEEQPER
Transmembrane helix 183-205 IYVLGIIYGVLLVMLIYNLFIFL
Inside 206-211 SVRDTS
Transmembrane helix 212-234 YLYYILYIASFGLYQVSVNGAGI
Outside 235-248 EYFWPDSPWWANAA
Transmembrane helix 249-268 TPFLIGSAALFGCQFARSFL
Inside 269-279 HTRDHSVWVDR
Transmembrane helix 280-302 GLLALMAVGALVMLMALTMSYAV
Outside 303-306 ALRL
Transmembrane helix 307-329 ATYLALAFTGLIFAAGILAWLRG
Inside 330-335 MRVARY
Transmembrane helix 336-358 FIIAWTAFLLGGIVNTLMVLGYL
Outside 359-795 PNMFLTMYASQIGSALEVGLLSLALADRINAMKEERARILQESSRKLEALNQELANSNRLKDEFLATVTHELRTPMSGVIGSLELMQTVPMDVELAEYQRTAAGSARDMMRMVNDILALIELQAGKLYPRREPFSLRGLFDSLRAQYAPRVEEKGLRFALQLDDSLPDTLEGDAGKLAQALGYLVDNAIKFTARGSVTLRVAAGRTHDGVALRVEVIDTGIGFDMAAGSDLYQRFVQADSSLTRGYGGLGIGLALCRKLVELLGGELTHESRPGQGSRFLLRLQLTQPAQGLAPPPRRAGGQAVRRPEECTVLVVEDNAINQLVTRGMLLKLGYRVRTADNGSEALELLARERPDGVLLDCQMPVMDGFATCRAIRALPGCAELPVLALTAHSHSGDRERCLAAGMSDYMAKPVKFEELQTLLHDWLLCQPIVTKSA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.7135 pos: 18-19. AGA-VS. MRHWLILFLLALPCLAGA


  Protein sequence of regulator: 796 a.a.    .

>REG0054 NC_002516:c4455676-4453289 [Pseudomonas aeruginosa PAO1]
MRHWLILFLLALPCLAGAVSFNEQVERLPLGQSIDVFEDVRGSADINDITSRAIDSSFRRHDKDVLNAGYSRSVFWLRLD
LDYRPVASSDPRTWLLELAYPPLDKLDLYLPDGQGGYRLAQRTGDTLPFASRPIRQNNYLFELGLEPNKPQRVYLRLESQ
GSIQAPLTLWSPKAYLEEQPERIYVLGIIYGVLLVMLIYNLFIFLSVRDTSYLYYILYIASFGLYQVSVNGAGIEYFWPD
SPWWANAATPFLIGSAALFGCQFARSFLHTRDHSVWVDRGLLALMAVGALVMLMALTMSYAVALRLATYLALAFTGLIFA
AGILAWLRGMRVARYFIIAWTAFLLGGIVNTLMVLGYLPNMFLTMYASQIGSALEVGLLSLALADRINAMKEERARILQE
SSRKLEALNQELANSNRLKDEFLATVTHELRTPMSGVIGSLELMQTVPMDVELAEYQRTAAGSARDMMRMVNDILALIEL
QAGKLYPRREPFSLRGLFDSLRAQYAPRVEEKGLRFALQLDDSLPDTLEGDAGKLAQALGYLVDNAIKFTARGSVTLRVA
AGRTHDGVALRVEVIDTGIGFDMAAGSDLYQRFVQADSSLTRGYGGLGIGLALCRKLVELLGGELTHESRPGQGSRFLLR
LQLTQPAQGLAPPPRRAGGQAVRRPEECTVLVVEDNAINQLVTRGMLLKLGYRVRTADNGSEALELLARERPDGVLLDCQ
MPVMDGFATCRAIRALPGCAELPVLALTAHSHSGDRERCLAAGMSDYMAKPVKFEELQTLLHDWLLCQPIVTKSA*

  Nucleotide sequence of regulator: 2388 bp    .

>REG0054 NC_002516:c4455676-4453289 [Pseudomonas aeruginosa PAO1]
ATGCGGCACTGGCTGATTCTCTTTCTTCTTGCGTTGCCGTGCCTGGCGGGTGCGGTGAGCTTCAACGAGCAGGTCGAGCG
CCTGCCGCTGGGGCAGTCCATCGACGTGTTCGAGGACGTGCGCGGTAGCGCCGATATCAACGACATCACCTCGCGGGCCA
TCGACAGCAGCTTCCGCCGCCATGACAAGGACGTGCTCAATGCCGGCTATTCGCGCTCGGTGTTCTGGTTGCGCCTGGAC
CTGGACTACCGGCCGGTCGCCTCCAGCGATCCGCGCACCTGGCTGCTGGAGCTGGCCTATCCGCCGCTGGACAAGCTCGA
CCTCTACCTGCCCGATGGCCAGGGCGGCTACCGCCTCGCCCAGCGCACCGGCGACACATTGCCGTTCGCCAGTCGGCCGA
TCCGGCAGAACAACTACCTGTTCGAACTCGGCCTCGAGCCGAACAAGCCGCAGCGTGTCTACCTGCGGCTGGAAAGCCAG
GGGTCGATCCAGGCGCCGCTGACCCTGTGGTCGCCCAAGGCCTACCTGGAGGAGCAGCCCGAACGCATCTATGTGCTCGG
CATCATCTATGGCGTGCTGCTGGTGATGCTGATCTACAACCTGTTCATCTTCCTCAGCGTCCGCGATACCAGCTATCTCT
ACTACATCCTCTATATCGCTTCGTTCGGGCTCTACCAGGTCTCGGTGAACGGCGCCGGCATCGAGTACTTCTGGCCCGAC
AGCCCCTGGTGGGCGAACGCCGCCACGCCATTCCTGATCGGTTCGGCGGCGCTGTTCGGCTGCCAGTTCGCGCGCAGCTT
CCTGCATACCCGCGACCACAGCGTGTGGGTCGACCGCGGCCTGCTGGCGCTGATGGCGGTCGGCGCGCTGGTCATGCTGA
TGGCGCTGACCATGAGCTACGCCGTGGCCCTGCGCCTGGCCACCTACCTGGCGCTGGCCTTCACCGGCCTGATCTTCGCC
GCCGGCATCCTCGCCTGGCTGCGCGGCATGCGCGTGGCGCGCTATTTCATCATCGCCTGGACCGCCTTCCTTCTCGGCGG
CATCGTCAACACCCTGATGGTCCTCGGCTACCTGCCGAACATGTTCCTCACCATGTATGCCAGCCAGATCGGTTCGGCGC
TGGAGGTGGGCTTGCTGTCGCTGGCACTGGCCGACCGGATCAACGCAATGAAGGAGGAGCGCGCGCGAATCCTCCAGGAG
TCCAGCCGCAAGCTGGAAGCGCTGAACCAGGAACTGGCCAACAGCAACCGCCTGAAGGACGAGTTCCTCGCCACCGTCAC
CCACGAACTGCGCACCCCGATGAGCGGGGTGATCGGTTCGCTGGAGCTGATGCAGACGGTGCCGATGGACGTCGAACTCG
CCGAGTACCAGCGCACCGCCGCCGGTTCGGCGCGGGACATGATGCGGATGGTCAACGACATCCTCGCCCTCATCGAACTG
CAGGCCGGCAAGCTCTACCCGCGCCGCGAGCCGTTCAGCCTGCGCGGCCTGTTCGACAGCCTGCGCGCGCAGTACGCGCC
GCGCGTCGAGGAGAAGGGCCTGCGCTTCGCCCTGCAACTGGACGACAGCCTGCCGGACACCCTCGAAGGTGATGCCGGCA
AGCTGGCCCAGGCGCTGGGCTACCTGGTCGACAACGCGATCAAGTTCACCGCCAGGGGCAGCGTGACCCTGCGCGTCGCC
GCCGGGCGTACCCATGACGGGGTGGCGCTGCGGGTGGAAGTGATCGACACCGGCATCGGTTTCGACATGGCCGCCGGCAG
CGACCTCTACCAGCGTTTCGTCCAGGCCGACAGCTCGTTGACCCGTGGCTACGGAGGCCTCGGCATCGGCCTGGCGCTGT
GCCGCAAGCTGGTGGAACTGCTTGGCGGCGAGCTGACCCACGAGTCCCGGCCGGGGCAGGGCAGCCGCTTCCTCCTGCGC
CTGCAACTGACCCAGCCGGCCCAGGGCCTGGCGCCGCCGCCGCGGCGCGCCGGCGGGCAGGCGGTGCGGCGTCCCGAGGA
GTGCACGGTGCTGGTGGTGGAGGACAACGCCATCAACCAGTTGGTGACTCGCGGCATGCTGCTCAAGCTCGGCTACCGGG
TGCGCACCGCCGACAACGGCAGCGAGGCCCTGGAGTTGCTGGCGCGCGAGCGGCCGGACGGCGTACTGCTCGACTGCCAG
ATGCCGGTGATGGACGGCTTCGCCACCTGTCGGGCGATCCGCGCGCTGCCCGGCTGCGCGGAACTGCCGGTGCTGGCGTT
GACCGCCCACAGCCACAGCGGCGATCGCGAGCGCTGCCTGGCGGCGGGCATGAGCGACTACATGGCCAAGCCGGTCAAGT
TCGAAGAGTTGCAGACCCTGCTGCACGATTGGCTGCTGTGCCAGCCGATCGTCACCAAGTCCGCCTGA