Detailed information of regulator


Summary

Regulator ID REG0053 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (943 a.a.); Nucleotide sequence (2829 bp)
Description In-gel digestion followed by tandem mass spectrometry identified the protein as Hcp1(PA0085), a result that was confirmed by deleting hcp1 in ΔretS.
Reference
External database links

Locus tag (Gene) PA4856 (RetS)
Coordinates (Strand) 5452150..5454978 (+)
NCBI ID 15600049
RefSeq NC_002516
Uniprot ID Q9HUV7_PSEAE
KEGG ID pae:PA4856
KEGG pathway (determined by BlastKOALA) map02025
PDB ID 2XBZ, 6DK8, 6DK7
  Target
Target Regulation (↓/↑) Target sequence
hcp1 (PA0085) expression and secretion ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
7TMR-DISM_7TM PF07695.13 1.3e-22 189..388
7TMR-DISMED2 PF07696.13 2.3e-22 48..170
Response_reg PF00072.26 4.7e-22 810..924
Response_reg PF00072.26 7.1e-19 661..776
HisKA PF00512.27 2.1e-18 415..479
HATPase_c PF02518.28 7.6e-16 526..640
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 VVRLRI
Transmembrane helix 7-26 AIGLLVSFLLLLLGPMSPAV
Outside 27-188 ADDAGVSSVPLQTTATTPSANQNWRLLRDESAQLRIADVLQRKEQFRPLAKRSFIFPASPQAVWLQVQLPAQKVPSWLWIFAPRVQYLDYYLVQDGQLVRDQHTGESRPFQERPLPSRSYLFSLPVDGKPMTLYVRMTSNHPLMAWFDQIDEAGLVGLEKPA
Transmembrane helix 189-211 YAFGMLLGGMLLLLMYNLIRFAY
Inside 212-217 SRSASS
Transmembrane helix 218-240 LWLAAVHAALAVCAAANLGLVAF
Outside 241-249 WLPGLKFNQ
Transmembrane helix 250-272 SLTADLGALGAAVSLLWFACSFF
Inside 273-304 RGTAESRLNRLLQGEALLILAVGAIIAFTQQL
Transmembrane helix 305-327 WFSWLIYLLVILSSLSVPLIAAW
Outside 328-336 HWYRGYQPA
Transmembrane helix 337-359 RLIVAGMIVFNAGFMVFLPVLFG
Inside 360-365 TKQLDP
Transmembrane helix 366-388 GWLVLGVFSFATLAGLVLSVSLT
Outside 389-942 ERQRLIQQLNLQQRTSEAAHTAELQTKAEFLAKISHEIRTPMNGVLGMTELLLGTPLSAKQRDYVQTIHSAGNELLTLINEILDISKLESGQIELDEVQFDLNALIEDCLDIFRVKAEQQRIELISFTQPQVPRVIGGDPTRLRQVVLSLLDNAFKQTEEGEILLVVALDDQGETPRLRIAVQDSGHPFDAKEREALLTAELHSGDFLSASKLGSHLGLIIARQLVRLMGGEFGIQSGSSQGTTLSLTLPLDPQQLENPTADLDGPLQGARLLVVDDNETCRKVLVQQCSGWGLNVSAVSSGKEALAQLRTKAHLREYFDVVLLDQDMPGMTGMQLAAKIKEDPNLNHDILLIMLTGISNAPSKIIARNAGIKRILAKPVAGYTLKATLADELAQRGVSGVTNYLQPAKEAQAPSLPSDFRILVAEDNSISTKVIRGMLNKLNLQPDTASNGQEALSAMKATQYDLVLMDCEMPVLDGFSATEQLRAWEAHEQRPHTPVVALTAHILSEHKERARLVGMDGHMAKPVELSQLRELIAYWVGERDRRRQGDALPS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9928 pos: 27-28. AVA-DD. VVRLRIAIGLLVSFLLLLLGPMSPAVA


  Protein sequence of regulator: 943 a.a.    .

>REG0053 NC_002516:5452150-5454978 [Pseudomonas aeruginosa PAO1]
VVRLRIAIGLLVSFLLLLLGPMSPAVADDAGVSSVPLQTTATTPSANQNWRLLRDESAQLRIADVLQRKEQFRPLAKRSF
IFPASPQAVWLQVQLPAQKVPSWLWIFAPRVQYLDYYLVQDGQLVRDQHTGESRPFQERPLPSRSYLFSLPVDGKPMTLY
VRMTSNHPLMAWFDQIDEAGLVGLEKPAYAFGMLLGGMLLLLMYNLIRFAYSRSASSLWLAAVHAALAVCAAANLGLVAF
WLPGLKFNQSLTADLGALGAAVSLLWFACSFFRGTAESRLNRLLQGEALLILAVGAIIAFTQQLWFSWLIYLLVILSSLS
VPLIAAWHWYRGYQPARLIVAGMIVFNAGFMVFLPVLFGTKQLDPGWLVLGVFSFATLAGLVLSVSLTERQRLIQQLNLQ
QRTSEAAHTAELQTKAEFLAKISHEIRTPMNGVLGMTELLLGTPLSAKQRDYVQTIHSAGNELLTLINEILDISKLESGQ
IELDEVQFDLNALIEDCLDIFRVKAEQQRIELISFTQPQVPRVIGGDPTRLRQVVLSLLDNAFKQTEEGEILLVVALDDQ
GETPRLRIAVQDSGHPFDAKEREALLTAELHSGDFLSASKLGSHLGLIIARQLVRLMGGEFGIQSGSSQGTTLSLTLPLD
PQQLENPTADLDGPLQGARLLVVDDNETCRKVLVQQCSGWGLNVSAVSSGKEALAQLRTKAHLREYFDVVLLDQDMPGMT
GMQLAAKIKEDPNLNHDILLIMLTGISNAPSKIIARNAGIKRILAKPVAGYTLKATLADELAQRGVSGVTNYLQPAKEAQ
APSLPSDFRILVAEDNSISTKVIRGMLNKLNLQPDTASNGQEALSAMKATQYDLVLMDCEMPVLDGFSATEQLRAWEAHE
QRPHTPVVALTAHILSEHKERARLVGMDGHMAKPVELSQLRELIAYWVGERDRRRQGDALPS*

  Nucleotide sequence of regulator: 2829 bp    .

>REG0053 NC_002516:5452150-5454978 [Pseudomonas aeruginosa PAO1]
GTGGTACGGCTTCGGATCGCCATAGGACTACTCGTCAGCTTCCTGCTGCTTCTGCTCGGTCCGATGTCGCCGGCGGTCGC
GGACGACGCCGGCGTTTCCAGCGTTCCCCTGCAAACCACCGCCACTACTCCCAGCGCCAACCAGAACTGGCGCCTGCTGC
GCGACGAGAGCGCGCAACTGCGGATCGCCGACGTCCTCCAGCGCAAGGAGCAGTTCCGCCCGCTGGCCAAGCGCTCCTTC
ATCTTCCCCGCCAGCCCCCAGGCGGTCTGGCTGCAGGTCCAGCTTCCCGCGCAGAAGGTGCCGAGCTGGCTGTGGATCTT
CGCCCCACGGGTGCAGTACCTGGACTACTACCTGGTGCAGGACGGCCAACTGGTGCGGGACCAGCACACCGGCGAGTCGC
GACCGTTCCAGGAGCGCCCGCTGCCCTCGCGCTCCTACCTGTTCTCGCTGCCGGTCGACGGCAAGCCGATGACCCTGTAC
GTGCGGATGACCTCCAACCATCCGCTGATGGCCTGGTTCGACCAGATCGACGAAGCCGGCCTGGTCGGCCTGGAGAAGCC
CGCCTACGCCTTCGGCATGCTGCTCGGCGGCATGCTCCTGCTGCTGATGTACAACCTGATCCGCTTCGCCTACTCGCGCA
GCGCCAGCAGCCTGTGGCTGGCGGCGGTGCACGCGGCGCTGGCGGTCTGCGCCGCGGCGAACCTGGGGCTGGTGGCGTTC
TGGCTGCCGGGACTCAAGTTCAACCAGTCGCTGACCGCCGACCTCGGCGCCCTCGGCGCCGCGGTGAGCCTGCTGTGGTT
CGCCTGCAGCTTCTTCCGCGGCACTGCGGAAAGCCGCCTGAACCGCCTCCTGCAAGGCGAGGCGCTGTTGATCCTGGCGG
TCGGCGCGATCATCGCCTTCACCCAGCAACTCTGGTTCAGTTGGCTGATCTACCTGCTGGTGATCCTCAGTTCGCTCAGC
GTCCCGCTGATCGCGGCCTGGCACTGGTATCGCGGCTACCAGCCGGCGCGCCTGATCGTTGCCGGGATGATCGTGTTCAA
CGCCGGCTTCATGGTGTTCCTGCCGGTACTGTTCGGCACCAAGCAACTCGATCCGGGCTGGCTGGTGCTCGGCGTGTTCA
GCTTCGCCACCCTCGCCGGGCTGGTCCTGAGCGTCTCGCTCACCGAACGCCAGCGGCTGATCCAGCAGCTCAACCTGCAA
CAGCGCACCAGCGAGGCGGCGCACACCGCGGAATTGCAGACCAAGGCCGAGTTCCTGGCCAAGATCAGCCACGAGATCCG
CACGCCCATGAACGGCGTGCTGGGCATGACCGAACTGCTCCTCGGCACCCCGTTGTCGGCGAAGCAGCGCGACTACGTGC
AGACCATCCACAGCGCCGGCAACGAGCTGCTCACGCTGATCAACGAGATACTCGACATCTCCAAGCTGGAGTCCGGGCAG
ATCGAGCTGGACGAAGTGCAGTTCGACCTCAACGCGTTGATCGAGGACTGCCTGGATATCTTCCGGGTCAAGGCCGAGCA
GCAGCGCATCGAGCTGATCAGCTTCACCCAGCCGCAGGTGCCGCGGGTCATCGGCGGCGATCCGACGCGCCTGCGCCAGG
TGGTCCTGAGCCTGCTGGACAACGCGTTCAAGCAGACCGAGGAAGGCGAGATCCTGCTGGTGGTGGCCCTCGACGACCAG
GGCGAGACGCCACGCCTGCGCATCGCCGTGCAGGACAGCGGCCACCCGTTCGACGCCAAGGAGCGCGAGGCGCTGCTCAC
CGCCGAGCTGCACAGCGGCGACTTCCTTTCCGCCAGCAAGCTCGGCAGCCACCTCGGCCTGATCATCGCCCGCCAACTGG
TGCGGCTGATGGGCGGGGAGTTCGGCATCCAGTCCGGCAGCAGCCAGGGCACCACCCTGTCGCTGACCCTGCCGCTCGAC
CCGCAGCAGCTGGAGAACCCCACCGCCGACCTCGACGGCCCGCTGCAGGGCGCCCGGCTGCTGGTGGTGGACGACAACGA
AACCTGCCGCAAGGTGCTGGTGCAGCAGTGCAGCGGCTGGGGCCTGAACGTCAGCGCGGTGTCGTCCGGCAAGGAGGCGC
TGGCCCAGTTGCGCACCAAGGCGCACCTGCGCGAGTACTTCGACGTGGTCCTGCTCGACCAGGACATGCCCGGCATGACC
GGCATGCAACTGGCGGCGAAGATCAAGGAAGACCCCAACCTCAACCACGACATCCTGCTGATCATGCTCACCGGGATCAG
CAACGCGCCGAGCAAGATCATCGCCCGCAACGCCGGGATCAAGCGCATCCTGGCCAAGCCGGTGGCCGGCTACACGCTGA
AGGCGACCCTCGCCGACGAACTCGCCCAGCGCGGCGTCAGCGGCGTCACCAACTACCTGCAACCGGCCAAGGAAGCGCAG
GCGCCGAGCCTGCCGAGCGACTTCCGGATCCTCGTCGCCGAGGACAACAGCATCTCCACCAAGGTCATCCGCGGCATGTT
GAACAAGCTCAACCTCCAGCCGGACACCGCCAGCAACGGCCAGGAGGCGCTCAGCGCGATGAAGGCGACCCAGTACGACC
TGGTGCTGATGGACTGCGAGATGCCGGTTCTGGACGGCTTCTCCGCCACCGAGCAGCTCCGCGCCTGGGAAGCCCACGAA
CAGCGCCCGCATACCCCGGTGGTGGCGCTCACCGCGCACATCCTCAGCGAACACAAGGAACGCGCGCGGCTGGTCGGCAT
GGACGGGCACATGGCCAAGCCGGTGGAGCTGTCGCAATTGCGTGAGCTGATCGCCTACTGGGTCGGCGAACGCGATCGCC
GCCGCCAGGGCGACGCCCTGCCCTCCTGA