Detailed information of regulator


Summary

Regulator ID REG0053 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (943 a.a.); Nucleotide sequence (2829 bp)
Description In-gel digestion followed by tandem mass spectrometry identified the protein as Hcp1(PA0085), a result that was confirmed by deleting hcp1 in ΔretS.
Reference
External database links

Locus tag (Gene) PA4856 (RetS)
Coordinates (Strand) 5452150..5454978 (+)
NCBI ID 15600049
RefSeq NC_002516
Uniprot ID Q9HUV7_PSEAE
KEGG ID pae:PA4856
KEGG pathway (determined by BlastKOALA) map02025
PDB ID 2XBZ, 6DK8, 6DK7
  Target
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Target
Regulation (↓/↑)
Target sequence
hcp1 (PA0085) expression and secretion↑-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
7TMR-DISM_7TMPF07695.131.3e-22189..388
7TMR-DISMED2PF07696.132.3e-2248..170
Response_regPF00072.264.7e-22810..924
Response_regPF00072.267.1e-19661..776
HisKAPF00512.272.1e-18415..479
HATPase_cPF02518.287.6e-16526..640
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Inside1-6VVRLRI
Transmembrane helix7-26AIGLLVSFLLLLLGPMSPAV
Outside27-188ADDAGVSSVPLQTTATTPSANQNWRLLRDESAQLRIADVLQRKEQFRPLAKRSFIFPASPQAVWLQVQLPAQKVPSWLWIFAPRVQYLDYYLVQDGQLVRDQHTGESRPFQERPLPSRSYLFSLPVDGKPMTLYVRMTSNHPLMAWFDQIDEAGLVGLEKPA
Transmembrane helix189-211YAFGMLLGGMLLLLMYNLIRFAY
Inside212-217SRSASS
Transmembrane helix218-240LWLAAVHAALAVCAAANLGLVAF
Outside241-249WLPGLKFNQ
Transmembrane helix250-272SLTADLGALGAAVSLLWFACSFF
Inside273-304RGTAESRLNRLLQGEALLILAVGAIIAFTQQL
Transmembrane helix305-327WFSWLIYLLVILSSLSVPLIAAW
Outside328-336HWYRGYQPA
Transmembrane helix337-359RLIVAGMIVFNAGFMVFLPVLFG
Inside360-365TKQLDP
Transmembrane helix366-388GWLVLGVFSFATLAGLVLSVSLT
Outside389-942ERQRLIQQLNLQQRTSEAAHTAELQTKAEFLAKISHEIRTPMNGVLGMTELLLGTPLSAKQRDYVQTIHSAGNELLTLINEILDISKLESGQIELDEVQFDLNALIEDCLDIFRVKAEQQRIELISFTQPQVPRVIGGDPTRLRQVVLSLLDNAFKQTEEGEILLVVALDDQGETPRLRIAVQDSGHPFDAKEREALLTAELHSGDFLSASKLGSHLGLIIARQLVRLMGGEFGIQSGSSQGTTLSLTLPLDPQQLENPTADLDGPLQGARLLVVDDNETCRKVLVQQCSGWGLNVSAVSSGKEALAQLRTKAHLREYFDVVLLDQDMPGMTGMQLAAKIKEDPNLNHDILLIMLTGISNAPSKIIARNAGIKRILAKPVAGYTLKATLADELAQRGVSGVTNYLQPAKEAQAPSLPSDFRILVAEDNSISTKVIRGMLNKLNLQPDTASNGQEALSAMKATQYDLVLMDCEMPVLDGFSATEQLRAWEAHEQRPHTPVVALTAHILSEHKERARLVGMDGHMAKPVELSQLRELIAYWVGERDRRRQGDALPS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
SP(Sec/SPI)0.9928pos: 27-28. AVA-DD.VVRLRIAIGLLVSFLLLLLGPMSPAVA


  Protein sequence of regulator: 943 a.a.    .

>REG0053 NC_002516:5452150-5454978 [Pseudomonas aeruginosa PAO1]
VVRLRIAIGLLVSFLLLLLGPMSPAVADDAGVSSVPLQTTATTPSANQNWRLLRDESAQLRIADVLQRKEQFRPLAKRSF
IFPASPQAVWLQVQLPAQKVPSWLWIFAPRVQYLDYYLVQDGQLVRDQHTGESRPFQERPLPSRSYLFSLPVDGKPMTLY
VRMTSNHPLMAWFDQIDEAGLVGLEKPAYAFGMLLGGMLLLLMYNLIRFAYSRSASSLWLAAVHAALAVCAAANLGLVAF
WLPGLKFNQSLTADLGALGAAVSLLWFACSFFRGTAESRLNRLLQGEALLILAVGAIIAFTQQLWFSWLIYLLVILSSLS
VPLIAAWHWYRGYQPARLIVAGMIVFNAGFMVFLPVLFGTKQLDPGWLVLGVFSFATLAGLVLSVSLTERQRLIQQLNLQ
QRTSEAAHTAELQTKAEFLAKISHEIRTPMNGVLGMTELLLGTPLSAKQRDYVQTIHSAGNELLTLINEILDISKLESGQ
IELDEVQFDLNALIEDCLDIFRVKAEQQRIELISFTQPQVPRVIGGDPTRLRQVVLSLLDNAFKQTEEGEILLVVALDDQ
GETPRLRIAVQDSGHPFDAKEREALLTAELHSGDFLSASKLGSHLGLIIARQLVRLMGGEFGIQSGSSQGTTLSLTLPLD
PQQLENPTADLDGPLQGARLLVVDDNETCRKVLVQQCSGWGLNVSAVSSGKEALAQLRTKAHLREYFDVVLLDQDMPGMT
GMQLAAKIKEDPNLNHDILLIMLTGISNAPSKIIARNAGIKRILAKPVAGYTLKATLADELAQRGVSGVTNYLQPAKEAQ
APSLPSDFRILVAEDNSISTKVIRGMLNKLNLQPDTASNGQEALSAMKATQYDLVLMDCEMPVLDGFSATEQLRAWEAHE
QRPHTPVVALTAHILSEHKERARLVGMDGHMAKPVELSQLRELIAYWVGERDRRRQGDALPS*

  Nucleotide sequence of regulator: 2829 bp    .

>REG0053 NC_002516:5452150-5454978 [Pseudomonas aeruginosa PAO1]
GTGGTACGGCTTCGGATCGCCATAGGACTACTCGTCAGCTTCCTGCTGCTTCTGCTCGGTCCGATGTCGCCGGCGGTCGC
GGACGACGCCGGCGTTTCCAGCGTTCCCCTGCAAACCACCGCCACTACTCCCAGCGCCAACCAGAACTGGCGCCTGCTGC
GCGACGAGAGCGCGCAACTGCGGATCGCCGACGTCCTCCAGCGCAAGGAGCAGTTCCGCCCGCTGGCCAAGCGCTCCTTC
ATCTTCCCCGCCAGCCCCCAGGCGGTCTGGCTGCAGGTCCAGCTTCCCGCGCAGAAGGTGCCGAGCTGGCTGTGGATCTT
CGCCCCACGGGTGCAGTACCTGGACTACTACCTGGTGCAGGACGGCCAACTGGTGCGGGACCAGCACACCGGCGAGTCGC
GACCGTTCCAGGAGCGCCCGCTGCCCTCGCGCTCCTACCTGTTCTCGCTGCCGGTCGACGGCAAGCCGATGACCCTGTAC
GTGCGGATGACCTCCAACCATCCGCTGATGGCCTGGTTCGACCAGATCGACGAAGCCGGCCTGGTCGGCCTGGAGAAGCC
CGCCTACGCCTTCGGCATGCTGCTCGGCGGCATGCTCCTGCTGCTGATGTACAACCTGATCCGCTTCGCCTACTCGCGCA
GCGCCAGCAGCCTGTGGCTGGCGGCGGTGCACGCGGCGCTGGCGGTCTGCGCCGCGGCGAACCTGGGGCTGGTGGCGTTC
TGGCTGCCGGGACTCAAGTTCAACCAGTCGCTGACCGCCGACCTCGGCGCCCTCGGCGCCGCGGTGAGCCTGCTGTGGTT
CGCCTGCAGCTTCTTCCGCGGCACTGCGGAAAGCCGCCTGAACCGCCTCCTGCAAGGCGAGGCGCTGTTGATCCTGGCGG
TCGGCGCGATCATCGCCTTCACCCAGCAACTCTGGTTCAGTTGGCTGATCTACCTGCTGGTGATCCTCAGTTCGCTCAGC
GTCCCGCTGATCGCGGCCTGGCACTGGTATCGCGGCTACCAGCCGGCGCGCCTGATCGTTGCCGGGATGATCGTGTTCAA
CGCCGGCTTCATGGTGTTCCTGCCGGTACTGTTCGGCACCAAGCAACTCGATCCGGGCTGGCTGGTGCTCGGCGTGTTCA
GCTTCGCCACCCTCGCCGGGCTGGTCCTGAGCGTCTCGCTCACCGAACGCCAGCGGCTGATCCAGCAGCTCAACCTGCAA
CAGCGCACCAGCGAGGCGGCGCACACCGCGGAATTGCAGACCAAGGCCGAGTTCCTGGCCAAGATCAGCCACGAGATCCG
CACGCCCATGAACGGCGTGCTGGGCATGACCGAACTGCTCCTCGGCACCCCGTTGTCGGCGAAGCAGCGCGACTACGTGC
AGACCATCCACAGCGCCGGCAACGAGCTGCTCACGCTGATCAACGAGATACTCGACATCTCCAAGCTGGAGTCCGGGCAG
ATCGAGCTGGACGAAGTGCAGTTCGACCTCAACGCGTTGATCGAGGACTGCCTGGATATCTTCCGGGTCAAGGCCGAGCA
GCAGCGCATCGAGCTGATCAGCTTCACCCAGCCGCAGGTGCCGCGGGTCATCGGCGGCGATCCGACGCGCCTGCGCCAGG
TGGTCCTGAGCCTGCTGGACAACGCGTTCAAGCAGACCGAGGAAGGCGAGATCCTGCTGGTGGTGGCCCTCGACGACCAG
GGCGAGACGCCACGCCTGCGCATCGCCGTGCAGGACAGCGGCCACCCGTTCGACGCCAAGGAGCGCGAGGCGCTGCTCAC
CGCCGAGCTGCACAGCGGCGACTTCCTTTCCGCCAGCAAGCTCGGCAGCCACCTCGGCCTGATCATCGCCCGCCAACTGG
TGCGGCTGATGGGCGGGGAGTTCGGCATCCAGTCCGGCAGCAGCCAGGGCACCACCCTGTCGCTGACCCTGCCGCTCGAC
CCGCAGCAGCTGGAGAACCCCACCGCCGACCTCGACGGCCCGCTGCAGGGCGCCCGGCTGCTGGTGGTGGACGACAACGA
AACCTGCCGCAAGGTGCTGGTGCAGCAGTGCAGCGGCTGGGGCCTGAACGTCAGCGCGGTGTCGTCCGGCAAGGAGGCGC
TGGCCCAGTTGCGCACCAAGGCGCACCTGCGCGAGTACTTCGACGTGGTCCTGCTCGACCAGGACATGCCCGGCATGACC
GGCATGCAACTGGCGGCGAAGATCAAGGAAGACCCCAACCTCAACCACGACATCCTGCTGATCATGCTCACCGGGATCAG
CAACGCGCCGAGCAAGATCATCGCCCGCAACGCCGGGATCAAGCGCATCCTGGCCAAGCCGGTGGCCGGCTACACGCTGA
AGGCGACCCTCGCCGACGAACTCGCCCAGCGCGGCGTCAGCGGCGTCACCAACTACCTGCAACCGGCCAAGGAAGCGCAG
GCGCCGAGCCTGCCGAGCGACTTCCGGATCCTCGTCGCCGAGGACAACAGCATCTCCACCAAGGTCATCCGCGGCATGTT
GAACAAGCTCAACCTCCAGCCGGACACCGCCAGCAACGGCCAGGAGGCGCTCAGCGCGATGAAGGCGACCCAGTACGACC
TGGTGCTGATGGACTGCGAGATGCCGGTTCTGGACGGCTTCTCCGCCACCGAGCAGCTCCGCGCCTGGGAAGCCCACGAA
CAGCGCCCGCATACCCCGGTGGTGGCGCTCACCGCGCACATCCTCAGCGAACACAAGGAACGCGCGCGGCTGGTCGGCAT
GGACGGGCACATGGCCAAGCCGGTGGAGCTGTCGCAATTGCGTGAGCTGATCGCCTACTGGGTCGGCGAACGCGATCGCC
GCCGCCAGGGCGACGCCCTGCCCTCCTGA