Detailed information of regulator


Summary

Regulator ID REG0038 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01041 insolico (Type i4b)
Strain Pseudomonas savastanoi pv. savastanoi NCPPB 3335
Replicon chromosome
Sequence Protein sequence (782 a.a.); Nucleotide sequence (2346 bp)
Description The DgcP‐mediated modulation of c‐di‐GMP levels plays a role in the regulation of T6SS‐related genes in both P. aeruginosa PAK and P. savastanoi pv. savastanoi NCPPB 3335.
Reference
External database links

Locus tag (Gene) PSA3335_06200 (dgcP)
Coordinates (Strand) 1344347..1346692 (+)
NCBI ID 488618048
RefSeq NZ_CP008742
Uniprot ID UPI0001D8D8C8
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
the levels of c-di-GMP ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
EAL PF00563.22 3.2e-73 525..759
GGDEF PF00990.23 1.2e-26 354..495
dCache_3 PF14827.8 1.5e-13 40..198
HAMP PF00672.27 9.7e-13 294..342
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKFRAS
Transmembrane helix 7-29 FQARIAGVLIVLLLIVVSAVYLA
Outside 30-781 VKVATDDAVRTQAQAQLEVGSRVFERLIDLRGKRLRDAVQLLAADFGFRDAVASADSSTIRSVLLNHGKRINASDMFLLGMDGVVVASTETAVPEGSRFVYDQALRNAKLAGQSVLIIPGGGIPHLLVESTVLAPLPIGRVVMGFTIDSGIAEELRSLSGLEVSFLTVENGQSGELISTQPKALYAGLIELMRSSSEGQMLLTEQANLNFLSQTLMLAKSSNGDDGQVIALLQSPLDKAYQAFAPLNEKISMIALLASLLGTLALARSVSLPVQELAIAAKRIGDGDYKTPVTLVRSDELGMLADAINTMQQGIAVREGQLAHNALHDNLTGLPNRALVMERLGSLIAANRTVALLYLGIENLATINESAGAEGVEQLLRQLGQRLQGNLRSGDTVARLSANEFLLLLDNTASDGAVGMADAVQRLLSEPQRIDNHDLMLECCIGITVYPEHGDSAQELVNRAAIARKDAAFIPGRLQIYQDGRDLAHQRQICLIRDLRKAPQNGELMLHYQPKLDIRQGYVRQAEALLRWTHPRFGNVSPAEFIVLAERTGSINLLTNWVIEEAMRQLAEWRQRGLVLQVSVNISADDLLGDDLAGYVMKLLKQYAVPAEQLVFEITESAVMSEPEKALVVLHRLRDCGISLSVDDFGTGYSSLAHLKRLPVQELKIDQSFVRNLDETSEDAVIVRSTIEMSHNLGLKVVAEGVEYQHSLDLLRRWHCDTAQGYLISRPLTASAFEAWIATYQASPSLMVN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9587 - -


  Protein sequence of regulator: 782 a.a.    .

>REG0038 NZ_CP008742:1344347-1346692 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MKFRASFQARIAGVLIVLLLIVVSAVYLAVKVATDDAVRTQAQAQLEVGSRVFERLIDLRGKRLRDAVQLLAADFGFRDA
VASADSSTIRSVLLNHGKRINASDMFLLGMDGVVVASTETAVPEGSRFVYDQALRNAKLAGQSVLIIPGGGIPHLLVEST
VLAPLPIGRVVMGFTIDSGIAEELRSLSGLEVSFLTVENGQSGELISTQPKALYAGLIELMRSSSEGQMLLTEQANLNFL
SQTLMLAKSSNGDDGQVIALLQSPLDKAYQAFAPLNEKISMIALLASLLGTLALARSVSLPVQELAIAAKRIGDGDYKTP
VTLVRSDELGMLADAINTMQQGIAVREGQLAHNALHDNLTGLPNRALVMERLGSLIAANRTVALLYLGIENLATINESAG
AEGVEQLLRQLGQRLQGNLRSGDTVARLSANEFLLLLDNTASDGAVGMADAVQRLLSEPQRIDNHDLMLECCIGITVYPE
HGDSAQELVNRAAIARKDAAFIPGRLQIYQDGRDLAHQRQICLIRDLRKAPQNGELMLHYQPKLDIRQGYVRQAEALLRW
THPRFGNVSPAEFIVLAERTGSINLLTNWVIEEAMRQLAEWRQRGLVLQVSVNISADDLLGDDLAGYVMKLLKQYAVPAE
QLVFEITESAVMSEPEKALVVLHRLRDCGISLSVDDFGTGYSSLAHLKRLPVQELKIDQSFVRNLDETSEDAVIVRSTIE
MSHNLGLKVVAEGVEYQHSLDLLRRWHCDTAQGYLISRPLTASAFEAWIATYQASPSLMVN*

  Nucleotide sequence of regulator: 2346 bp    .

>REG0038 NZ_CP008742:1344347-1346692 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGAAATTTAGAGCCAGCTTTCAGGCACGCATTGCCGGGGTTCTGATTGTCCTGTTGCTGATTGTCGTGAGTGCGGTCTA
TCTCGCCGTCAAGGTTGCGACCGACGACGCGGTGCGCACTCAGGCCCAGGCGCAGCTGGAAGTGGGTAGCCGTGTGTTCG
AGCGGCTGATTGATCTGCGCGGCAAGCGTCTGCGTGACGCCGTGCAACTGTTGGCGGCCGACTTCGGGTTTCGTGATGCT
GTAGCGAGTGCCGATTCATCGACCATTCGATCGGTACTGCTCAATCATGGCAAACGCATCAACGCCAGCGATATGTTCCT
GCTGGGCATGGATGGCGTGGTGGTTGCCAGTACCGAGACAGCGGTGCCGGAGGGGTCCAGGTTTGTCTATGACCAGGCGT
TGCGCAACGCCAAGCTTGCCGGTCAGTCAGTGCTTATTATTCCGGGTGGCGGAATACCTCATTTGCTGGTGGAAAGTACC
GTGCTTGCGCCGTTGCCGATAGGCCGCGTAGTGATGGGCTTCACCATCGACAGTGGCATTGCCGAAGAGCTGCGTTCTCT
CAGTGGGCTGGAAGTGTCGTTTCTCACGGTGGAAAACGGCCAGTCCGGGGAACTGATCAGCACCCAGCCGAAAGCCTTGT
ACGCTGGTCTGATCGAGCTGATGCGCAGTTCCTCTGAAGGGCAGATGCTGCTGACCGAGCAAGCGAACCTCAACTTTCTC
AGTCAAACGCTGATGCTGGCCAAAAGCAGCAATGGCGACGACGGACAAGTGATCGCGCTGTTGCAGAGCCCGCTGGACAA
GGCTTATCAAGCGTTTGCACCGCTGAATGAAAAGATTTCCATGATCGCATTGCTGGCGTCGCTGCTTGGTACGCTGGCGC
TGGCCCGCAGCGTGTCGCTGCCGGTGCAGGAGTTGGCTATTGCGGCCAAACGTATTGGCGATGGCGACTATAAAACGCCG
GTAACCCTGGTGCGCAGTGACGAATTGGGCATGCTCGCCGACGCGATCAACACCATGCAACAGGGTATCGCCGTGCGCGA
AGGCCAGTTGGCTCACAACGCTCTGCACGACAACCTGACCGGCCTGCCCAATCGAGCGTTGGTGATGGAACGTCTTGGCA
GTTTGATTGCGGCAAATCGCACGGTCGCCTTGCTGTACCTAGGCATCGAGAACCTGGCCACGATCAACGAAAGTGCGGGT
GCCGAAGGCGTTGAGCAACTGTTACGCCAGTTGGGGCAGCGTTTGCAGGGCAATTTGCGGTCTGGCGACACGGTGGCACG
GCTGAGTGCCAACGAGTTCTTGCTGTTGCTGGACAACACTGCCAGTGACGGCGCGGTGGGCATGGCCGACGCCGTGCAGC
GCTTGCTCAGCGAGCCGCAACGTATCGACAACCATGATCTGATGCTTGAGTGCTGCATAGGCATCACGGTTTATCCGGAA
CACGGTGATTCGGCTCAGGAACTGGTCAACCGTGCGGCGATTGCACGCAAGGACGCAGCGTTCATTCCCGGTCGCCTGCA
GATCTACCAGGACGGACGTGACCTCGCTCACCAGCGTCAGATATGTCTGATCCGCGACCTGCGCAAGGCTCCGCAGAATG
GCGAACTGATGCTCCATTACCAACCCAAACTGGATATCCGTCAGGGCTATGTTCGTCAGGCCGAAGCCCTGCTGCGCTGG
ACTCACCCGCGCTTTGGCAATGTTTCGCCAGCCGAATTCATTGTGCTCGCCGAGCGCACCGGCAGTATCAACCTGCTGAC
CAACTGGGTTATCGAAGAGGCAATGCGTCAGTTGGCCGAATGGCGCCAGCGCGGTCTGGTGCTGCAGGTATCGGTCAACA
TTTCCGCCGACGACCTGCTGGGCGACGATCTGGCCGGTTATGTCATGAAACTGCTCAAGCAATACGCGGTGCCTGCCGAA
CAACTGGTATTCGAGATAACCGAGAGCGCGGTCATGAGCGAGCCGGAGAAAGCCCTGGTGGTTCTGCATCGTCTACGCGA
CTGCGGTATCAGCCTGTCGGTCGATGACTTCGGCACCGGTTACTCGTCACTGGCCCACTTGAAGCGTCTGCCAGTGCAGG
AGTTGAAAATCGACCAGTCTTTCGTGCGCAACCTGGATGAAACCAGCGAAGACGCGGTGATCGTGCGGTCGACCATCGAA
ATGAGCCATAACCTCGGACTCAAGGTGGTGGCAGAGGGTGTCGAGTACCAACACAGTCTGGACCTGCTCCGGCGTTGGCA
TTGCGATACGGCCCAGGGTTACCTGATCAGTCGGCCGCTGACGGCGTCAGCATTCGAGGCATGGATTGCTACGTATCAGG
CATCACCCAGTCTTATGGTGAATTAA