Detailed information of regulator

Summary

Regulator ID	REG0004
Regulator type	protein
Regulatory pathway (from literatures)	TCS-RR
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Biofilm formation - Escherichia coli (map02026)
Related T6SS	T6SS01264 (Type i3)
Strain	Serratia marcescens strain RM66262
Replicon	chromosome
Sequence	Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Description	RcsB is involved in the control of Serratia T6SS expression and DNase I protection assay is performed on coding strand and the consensus motif for the RcsB-binding site is indicated in red.
Reference	[1] Lazzaro M, Feldman MF, García Véscovi E. A Transcriptional Regulatory Mechanism Finely Tunes the Firing of Type VI Secretion System in Response to Bacterial Enemies. mBio. 2017 Aug 22;8(4). pii: e00559-17. doi: 10.1128/mBio.00559-17. PMID: 28830939

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External database links

Locus tag (Gene)	RT90_01115 (rcsB)
Coordinates (Strand)	235561..236211 (-)
NCBI ID	735006328
GenBank	JWLO01000001
Uniprot ID	UPI00044EB8A6
KEGG ID	-
KEGG pathway (determined by BlastKOALA)	map02020; map02026
PDB ID	-

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  Target

Target	Regulation (↓/↑)	Target sequence
vgrG promoter	↓	5'-AAATAGGAATCATCCGAATTAACATT-3' 3'-CGGTTTATCCTTAGTAGGCTTA-5'

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Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	7.1e-25	6..120
GerE	PF00196.21	3.4e-12	151..201

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-216	MNNLNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLSKLDANVLITDLSMPGDKYGDGITLIKYIKRHYPQLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGAPTDLPKALAALQKGKKFTPESVSKLLEKISASGYGDKRLSPKESEVLRLFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVSMTPLDKD

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9976	-	-

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  Protein sequence of regulator: 217 a.a.    .Download


  Nucleotide sequence of regulator: 651 bp    .Download