Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00383  |
| Cognate effector | EFF01894 |
| Related T6SS (Type) | T6SS01301 (Type i3) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (236 a.a.); Nucleotide sequence (708 bp) |
| Description | Immunity protein represses the expression of the operon via binding to both external and internal promoters. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ACI79_RS12840 (TsiTBg1) |
| Coordinates (Strand) | 27037..27744 (-) |
| NCBI ID | 836689521 |
| GenBank | LEKY01000011 |
| Uniprot ID | UPI00064A9F14 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Imm52 | PF15579.8 | 1.1e-34 | 130..229 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-235 | MKITSRFRDPLHDASNLPTVFEQLGAIAEAIHASAPRFAQWFLTGDSKEEAHLYPAFENWTPTTAALAVLRTEFEGAEHRPQIISLWDGGDDQRTGAVLTYLVAAKERPKKIEADFNDEREFSAEQVAAIVDTIARQTNPAYVTVEPQGYADKQVFDDKPGVGWMLYLPKPITSQQVPEAHALLPVTIDGKQIGTIIVSVVDAPFSMDNSEHVETANRIEIRLVDQDLLPRYTDI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9800 | - | - |
Protein sequence: 236 a.a. .
>IMU00383 LEKY01000011:c27744-27037 [Burkholderia gladioli]
MKITSRFRDPLHDASNLPTVFEQLGAIAEAIHASAPRFAQWFLTGDSKEEAHLYPAFENWTPTTAALAVLRTEFEGAEHR
PQIISLWDGGDDQRTGAVLTYLVAAKERPKKIEADFNDEREFSAEQVAAIVDTIARQTNPAYVTVEPQGYADKQVFDDKP
GVGWMLYLPKPITSQQVPEAHALLPVTIDGKQIGTIIVSVVDAPFSMDNSEHVETANRIEIRLVDQDLLPRYTDI*
MKITSRFRDPLHDASNLPTVFEQLGAIAEAIHASAPRFAQWFLTGDSKEEAHLYPAFENWTPTTAALAVLRTEFEGAEHR
PQIISLWDGGDDQRTGAVLTYLVAAKERPKKIEADFNDEREFSAEQVAAIVDTIARQTNPAYVTVEPQGYADKQVFDDKP
GVGWMLYLPKPITSQQVPEAHALLPVTIDGKQIGTIIVSVVDAPFSMDNSEHVETANRIEIRLVDQDLLPRYTDI*
Nucleotide sequence: 708 bp .
>IMU00383 LEKY01000011:c27744-27037 [Burkholderia gladioli]
ATGAAAATCACTTCCCGCTTTCGAGATCCGCTGCATGATGCATCGAACCTGCCAACCGTCTTCGAACAACTCGGAGCGAT
CGCCGAGGCGATACATGCTAGCGCCCCCCGGTTCGCTCAATGGTTCCTGACCGGCGACTCAAAAGAAGAAGCACATCTGT
ATCCAGCGTTTGAGAACTGGACTCCAACGACTGCCGCACTAGCAGTATTGCGGACCGAGTTTGAAGGAGCAGAGCACCGA
CCGCAGATCATCTCTTTATGGGACGGTGGCGACGATCAACGCACGGGAGCGGTATTAACCTATCTCGTTGCAGCCAAGGA
GCGACCGAAAAAGATCGAAGCCGACTTCAACGACGAGCGCGAATTTTCCGCCGAGCAAGTCGCTGCAATCGTCGATACTA
TCGCCCGACAGACTAACCCGGCATACGTGACCGTCGAGCCGCAGGGTTATGCCGACAAGCAGGTGTTCGACGACAAACCC
GGCGTCGGATGGATGCTGTACCTGCCAAAGCCAATCACGTCGCAACAGGTGCCCGAAGCACATGCCCTTTTGCCCGTTAC
CATAGACGGGAAGCAGATCGGTACAATCATCGTCAGCGTAGTAGACGCCCCATTCTCGATGGACAACTCCGAACATGTCG
AGACAGCGAACCGTATCGAAATTCGATTAGTTGACCAAGATCTTTTACCGAGATACACGGACATCTGA
ATGAAAATCACTTCCCGCTTTCGAGATCCGCTGCATGATGCATCGAACCTGCCAACCGTCTTCGAACAACTCGGAGCGAT
CGCCGAGGCGATACATGCTAGCGCCCCCCGGTTCGCTCAATGGTTCCTGACCGGCGACTCAAAAGAAGAAGCACATCTGT
ATCCAGCGTTTGAGAACTGGACTCCAACGACTGCCGCACTAGCAGTATTGCGGACCGAGTTTGAAGGAGCAGAGCACCGA
CCGCAGATCATCTCTTTATGGGACGGTGGCGACGATCAACGCACGGGAGCGGTATTAACCTATCTCGTTGCAGCCAAGGA
GCGACCGAAAAAGATCGAAGCCGACTTCAACGACGAGCGCGAATTTTCCGCCGAGCAAGTCGCTGCAATCGTCGATACTA
TCGCCCGACAGACTAACCCGGCATACGTGACCGTCGAGCCGCAGGGTTATGCCGACAAGCAGGTGTTCGACGACAAACCC
GGCGTCGGATGGATGCTGTACCTGCCAAAGCCAATCACGTCGCAACAGGTGCCCGAAGCACATGCCCTTTTGCCCGTTAC
CATAGACGGGAAGCAGATCGGTACAATCATCGTCAGCGTAGTAGACGCCCCATTCTCGATGGACAACTCCGAACATGTCG
AGACAGCGAACCGTATCGAAATTCGATTAGTTGACCAAGATCTTTTACCGAGATACACGGACATCTGA