Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00359  |
| Cognate effector | EFF01441  |
| Related T6SS (Type) | T6SS01224 (Type i1) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (217 a.a.); Nucleotide sequence (651 bp) |
| Description | Strains deleted for hcp1 or v12G01_02265 were no longer toxic toward the Δv12G01_02265-v12G01_02260 strain, indicating that self-intoxication was mediated by T6SS1, and specifically by v12G01_02265 |
| Reference |
Experimental investigation has been performed. Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | V12G01_RS26200 |
| Coordinates (Strand) | 308691..309341 (-) |
| NCBI ID | 91190980 |
| RefSeq | NZ_CH902589 |
| Uniprot ID | UPI0000D52D64 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-216 | MKYRFLIAVSMCFLLNGCFESKPVSVASIGNAGSLSGPHFIRDMWVSDIYGGGANFAYGSVQGCCASASIGIGIPSAIKGKWENGVGGGSSREKWQKRWKPEWGNFDENYDTKWFRIDDAIDSELAKKKIETMNHYYKNHHANPVMKLLVNNDRVLLLYAYGCTPEINDDCTVREGADPNGWVQVAPYSKHKNSTVVVLYEGRGEVSDKPFTDNTP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9914 | pos: 17-18. LNG-CF. | MKYRFLIAVSMCFLLNG |
Protein sequence: 217 a.a. .
>IMU00359 NZ_CH902589:c309341-308691 [Vibrio alginolyticus 12G01]
MKYRFLIAVSMCFLLNGCFESKPVSVASIGNAGSLSGPHFIRDMWVSDIYGGGANFAYGSVQGCCASASIGIGIPSAIKG
KWENGVGGGSSREKWQKRWKPEWGNFDENYDTKWFRIDDAIDSELAKKKIETMNHYYKNHHANPVMKLLVNNDRVLLLYA
YGCTPEINDDCTVREGADPNGWVQVAPYSKHKNSTVVVLYEGRGEVSDKPFTDNTP*
MKYRFLIAVSMCFLLNGCFESKPVSVASIGNAGSLSGPHFIRDMWVSDIYGGGANFAYGSVQGCCASASIGIGIPSAIKG
KWENGVGGGSSREKWQKRWKPEWGNFDENYDTKWFRIDDAIDSELAKKKIETMNHYYKNHHANPVMKLLVNNDRVLLLYA
YGCTPEINDDCTVREGADPNGWVQVAPYSKHKNSTVVVLYEGRGEVSDKPFTDNTP*
Nucleotide sequence: 651 bp .
>IMU00359 NZ_CH902589:c309341-308691 [Vibrio alginolyticus 12G01]
ATGAAGTATAGATTTTTAATTGCTGTAAGCATGTGCTTTTTGCTTAATGGTTGCTTTGAGAGTAAGCCAGTTTCAGTTGC
GAGCATTGGCAATGCAGGGTCTTTAAGCGGTCCTCATTTTATTCGTGATATGTGGGTCAGTGATATATATGGTGGAGGTG
CAAACTTTGCGTATGGGAGCGTTCAGGGCTGTTGTGCTAGCGCAAGCATTGGCATTGGTATCCCTAGCGCCATAAAAGGT
AAATGGGAAAATGGAGTTGGTGGCGGCTCATCCCGTGAAAAGTGGCAAAAGCGTTGGAAGCCCGAATGGGGTAATTTCGA
TGAGAATTATGATACTAAATGGTTTCGAATTGATGATGCGATAGATAGTGAGCTTGCCAAGAAAAAAATTGAAACAATGA
ACCATTACTACAAGAACCATCATGCAAACCCAGTCATGAAGCTTCTTGTAAATAATGATAGGGTCTTACTTTTGTACGCT
TACGGCTGTACTCCAGAAATAAACGATGACTGCACGGTCAGAGAGGGAGCAGACCCTAATGGATGGGTTCAAGTAGCACC
GTATAGCAAGCATAAGAACTCAACTGTGGTTGTTCTTTATGAAGGCAGAGGTGAGGTCTCAGACAAACCATTTACGGATA
ATACACCTTAG
ATGAAGTATAGATTTTTAATTGCTGTAAGCATGTGCTTTTTGCTTAATGGTTGCTTTGAGAGTAAGCCAGTTTCAGTTGC
GAGCATTGGCAATGCAGGGTCTTTAAGCGGTCCTCATTTTATTCGTGATATGTGGGTCAGTGATATATATGGTGGAGGTG
CAAACTTTGCGTATGGGAGCGTTCAGGGCTGTTGTGCTAGCGCAAGCATTGGCATTGGTATCCCTAGCGCCATAAAAGGT
AAATGGGAAAATGGAGTTGGTGGCGGCTCATCCCGTGAAAAGTGGCAAAAGCGTTGGAAGCCCGAATGGGGTAATTTCGA
TGAGAATTATGATACTAAATGGTTTCGAATTGATGATGCGATAGATAGTGAGCTTGCCAAGAAAAAAATTGAAACAATGA
ACCATTACTACAAGAACCATCATGCAAACCCAGTCATGAAGCTTCTTGTAAATAATGATAGGGTCTTACTTTTGTACGCT
TACGGCTGTACTCCAGAAATAAACGATGACTGCACGGTCAGAGAGGGAGCAGACCCTAATGGATGGGTTCAAGTAGCACC
GTATAGCAAGCATAAGAACTCAACTGTGGTTGTTCTTTATGAAGGCAGAGGTGAGGTCTCAGACAAACCATTTACGGATA
ATACACCTTAG