Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00355  |
| Cognate effector | EFF01838 |
| Related T6SS (Type) | T6SS00014 (Type i4b) |
| Strain | Pseudomonas putida KT2440 |
| Replicon | chromosome |
| Sequence | Protein sequence (159 a.a.); Nucleotide sequence (477 bp) |
| Description | Tke2 is an effector of the K1-T6SS and its activity is antagonized by the Tki2 immunity protein |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PP_5576 (Tki2) |
| Coordinates (Strand) | 3515484..3515960 (+) |
| NCBI ID | 898330862 |
| RefSeq | NC_002947 |
| Uniprot ID | A0A140FWC7_PSEPK, A0A2V4G1S7_9PSED |
| KEGG ID | ppu:PP_5576 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-158 | MVINGGSLAENLVGSSAYRELLAELVDATGIEVESEYAEVFARLDSAKIVKAKVDIDDLMSILTNKMDLIKQYSICKFQALSDEEDEEEYPVGAEPSGDERSKTLSVGKYSQGFLLTNLIEFALAMSGRECLLEYIKLCRIPNAKKYTDQIIKLTGLG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9936 | - | - |
Protein sequence: 159 a.a. .
>IMU00355 NC_002947:3515484-3515960 [Pseudomonas putida KT2440]
MVINGGSLAENLVGSSAYRELLAELVDATGIEVESEYAEVFARLDSAKIVKAKVDIDDLMSILTNKMDLIKQYSICKFQA
LSDEEDEEEYPVGAEPSGDERSKTLSVGKYSQGFLLTNLIEFALAMSGRECLLEYIKLCRIPNAKKYTDQIIKLTGLG*
MVINGGSLAENLVGSSAYRELLAELVDATGIEVESEYAEVFARLDSAKIVKAKVDIDDLMSILTNKMDLIKQYSICKFQA
LSDEEDEEEYPVGAEPSGDERSKTLSVGKYSQGFLLTNLIEFALAMSGRECLLEYIKLCRIPNAKKYTDQIIKLTGLG*
Nucleotide sequence: 477 bp .
>IMU00355 NC_002947:3515484-3515960 [Pseudomonas putida KT2440]
ATGGTAATCAATGGCGGTTCATTGGCGGAGAATCTAGTTGGGTCGTCTGCTTATAGGGAGTTACTTGCGGAGCTAGTCGA
CGCTACAGGTATTGAGGTTGAGTCGGAATATGCAGAAGTATTCGCTCGTCTCGACTCTGCTAAGATTGTAAAGGCAAAGG
TTGATATTGATGACTTGATGTCCATATTGACAAACAAAATGGATCTTATAAAGCAGTATTCTATTTGTAAGTTCCAAGCG
CTTTCAGATGAGGAGGATGAGGAAGAGTATCCCGTTGGCGCAGAGCCTTCTGGCGATGAAAGAAGTAAAACTCTTTCGGT
TGGGAAATATTCACAAGGTTTTTTGTTGACCAATCTTATTGAGTTTGCATTGGCGATGTCTGGTCGTGAATGCCTGCTGG
AATATATTAAATTGTGTAGAATCCCAAATGCAAAAAAATATACTGATCAAATAATCAAGTTGACAGGTCTTGGGTAG
ATGGTAATCAATGGCGGTTCATTGGCGGAGAATCTAGTTGGGTCGTCTGCTTATAGGGAGTTACTTGCGGAGCTAGTCGA
CGCTACAGGTATTGAGGTTGAGTCGGAATATGCAGAAGTATTCGCTCGTCTCGACTCTGCTAAGATTGTAAAGGCAAAGG
TTGATATTGATGACTTGATGTCCATATTGACAAACAAAATGGATCTTATAAAGCAGTATTCTATTTGTAAGTTCCAAGCG
CTTTCAGATGAGGAGGATGAGGAAGAGTATCCCGTTGGCGCAGAGCCTTCTGGCGATGAAAGAAGTAAAACTCTTTCGGT
TGGGAAATATTCACAAGGTTTTTTGTTGACCAATCTTATTGAGTTTGCATTGGCGATGTCTGGTCGTGAATGCCTGCTGG
AATATATTAAATTGTGTAGAATCCCAAATGCAAAAAAATATACTGATCAAATAATCAAGTTGACAGGTCTTGGGTAG