Detailed information of immunity protein


Summary

Immunity protein ID IMU00350 experimental
Cognate effector EFF01868 experimental
Related T6SS (Type) T6SS01103 experimental (Type i5)
Strain Agrobacterium tumefaciens strain 1D1609
Replicon chromosome
Sequence Protein sequence (367 a.a.); Nucleotide sequence (1101 bp)
Description The plasmid degradation caused by nuclease activity of V2a is no longer detectable when the cells expressing the catalytic site (H385A and H386A) mutant or co-expression of v3a and bacterial growth is inhibited when v2a is induced as compared to the vector control whereas co-expression with v3a restores the growth
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) At1D1609_44750 (v3a)
Coordinates (Strand) 1638588..1639688 (+)
NCBI ID 1349686714
RefSeq NZ_CP026925
Uniprot ID A0A2L2LJL9_RHIRD
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-366 MKTKLPIDFTRAYARSGKTLAVVATRDNAEPFDPDGRFYRYDRDDVPPQDWTYSDFGFHIQSMTVYHSADHATPDGYFVLLSSEGDVYHTYWKENFQEKIQGAGSWSDDSKGYGRLTRIREIGQHLYACGEGGQIYVRKGNNDWRLLTDKLLLNPNAQEEAKPEKEPEFLSPEWQEWVTRSALNPASRDILFFDINGLSEEAIYLCGEEGPGTKPVLCFWDGHELHELKIPIPVAALTGIYIENPDSVWICGREGVLIHGSHARGFSPVALRQQHNLFHMITPYREKLVMAASVRPGGLYEYDPKTGRFGRFDPPLPRLSNRDDNESIDNGPFFAQVIENVLWVVASKDVFRFDGEAWERIEHPDL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8749 - -


  Protein sequence: 367 a.a.    .

>IMU00350 NZ_CP026925:1638588-1639688 [Agrobacterium tumefaciens]
MKTKLPIDFTRAYARSGKTLAVVATRDNAEPFDPDGRFYRYDRDDVPPQDWTYSDFGFHIQSMTVYHSADHATPDGYFVL
LSSEGDVYHTYWKENFQEKIQGAGSWSDDSKGYGRLTRIREIGQHLYACGEGGQIYVRKGNNDWRLLTDKLLLNPNAQEE
AKPEKEPEFLSPEWQEWVTRSALNPASRDILFFDINGLSEEAIYLCGEEGPGTKPVLCFWDGHELHELKIPIPVAALTGI
YIENPDSVWICGREGVLIHGSHARGFSPVALRQQHNLFHMITPYREKLVMAASVRPGGLYEYDPKTGRFGRFDPPLPRLS
NRDDNESIDNGPFFAQVIENVLWVVASKDVFRFDGEAWERIEHPDL*

  Nucleotide sequence: 1101 bp    .

>IMU00350 NZ_CP026925:1638588-1639688 [Agrobacterium tumefaciens]
ATGAAAACCAAGCTGCCTATCGATTTCACGAGAGCATATGCGAGGTCGGGCAAAACGCTCGCCGTTGTCGCAACGCGAGA
CAATGCTGAACCTTTCGATCCTGATGGCCGATTTTATCGGTATGATCGTGACGACGTGCCGCCGCAGGACTGGACGTATT
CCGACTTCGGTTTCCATATTCAAAGCATGACTGTGTATCATAGCGCTGACCACGCTACGCCAGATGGTTATTTCGTTTTG
CTGTCGTCAGAGGGTGATGTTTATCACACCTACTGGAAGGAGAATTTTCAAGAGAAAATTCAGGGAGCCGGTAGTTGGAG
TGACGATTCGAAAGGCTACGGGCGTCTCACTCGCATCCGCGAGATCGGGCAACACCTATATGCTTGCGGAGAGGGGGGGC
AAATCTATGTTCGCAAGGGGAACAATGATTGGCGTCTCCTGACCGATAAATTGTTGCTGAACCCAAATGCGCAGGAGGAA
GCAAAGCCTGAAAAAGAGCCGGAATTTCTTTCTCCGGAATGGCAGGAATGGGTCACTCGGTCGGCCCTCAATCCAGCCTC
CCGCGATATACTGTTCTTTGACATAAACGGGTTGTCGGAAGAAGCCATCTATCTTTGCGGGGAGGAGGGGCCCGGTACCA
AACCGGTCCTTTGTTTCTGGGATGGTCATGAGTTGCACGAACTGAAAATTCCAATTCCGGTAGCCGCTTTGACCGGAATT
TACATCGAGAATCCTGACAGTGTCTGGATATGTGGGAGAGAAGGTGTTCTCATACACGGAAGTCACGCGCGGGGATTTTC
GCCTGTCGCATTACGGCAGCAGCATAATCTCTTTCACATGATCACTCCGTATCGGGAAAAACTTGTCATGGCGGCGAGCG
TTCGTCCCGGCGGTCTTTATGAGTATGATCCCAAGACAGGACGTTTTGGCAGATTTGATCCTCCTCTTCCGCGCTTGAGC
AATCGTGATGATAACGAATCTATCGACAATGGTCCGTTTTTTGCGCAGGTGATCGAAAACGTGCTTTGGGTCGTCGCATC
GAAAGATGTTTTCCGCTTCGATGGTGAGGCTTGGGAGCGGATAGAACACCCAGATCTCTGA