Detailed information of immunity protein


Summary

Immunity protein ID IMU00332 experimental
Cognate effector EFF01495 experimental
Related T6SS (Type) -
Strain Pseudomonas putida B6-2
Replicon chromosome
Sequence Protein sequence (372 a.a.); Nucleotide sequence (1116 bp)
Description Heterologous expression of Tri1 from P. putida B6-2 also increased the competitiveness of E. coli grown in co-culture with S. proteamaculans to a similar degree as expression of Tri1-Sp
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) KKK_RS13285 (Tri1)
Coordinates (Strand) 2922386..2923501 (-)
NCBI ID 657294452
RefSeq NZ_CP015202
Uniprot ID UPI0002D342D6
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ADP_ribosyl_GH PF03747.16 1.1e-55 70..334
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-371 MIDLRSPDAILSDYAKRYAHLLPELSPQLQQRMAYMPEPDAPRLPLTKPAWLSDRSCTLTPGQAIDRAQGALLGLAIGDAVGTTLEFRRRDQGQVSDMVGGGPFRLAPGEWTDDTSMALCLADTYASQGKFDFATFADAMVRWYRQGENSANGRCFDIGNVTRRALEGWEAQGLAWMGNLEASTAGNGSLIRLAPTAIFRRHSLSATWWESVTQSSVTHRAIEVSECCKLFGAQLHLALNGADKEEALSPKVRPLQPRALIINAGEYKHKTREQIRSSGYVVDTLEAALWAVWNTDNFRDAILLAANLADDADSVAATAGQIAGALYGVSGMPPEWVAKVAWSRHICELASRLFELAPQGDEMDELLSGSE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9868 - -


  Protein sequence: 372 a.a.    .

>IMU00332 NZ_CP015202:c2923501-2922386 [Pseudomonas putida B6-2]
MIDLRSPDAILSDYAKRYAHLLPELSPQLQQRMAYMPEPDAPRLPLTKPAWLSDRSCTLTPGQAIDRAQGALLGLAIGDA
VGTTLEFRRRDQGQVSDMVGGGPFRLAPGEWTDDTSMALCLADTYASQGKFDFATFADAMVRWYRQGENSANGRCFDIGN
VTRRALEGWEAQGLAWMGNLEASTAGNGSLIRLAPTAIFRRHSLSATWWESVTQSSVTHRAIEVSECCKLFGAQLHLALN
GADKEEALSPKVRPLQPRALIINAGEYKHKTREQIRSSGYVVDTLEAALWAVWNTDNFRDAILLAANLADDADSVAATAG
QIAGALYGVSGMPPEWVAKVAWSRHICELASRLFELAPQGDEMDELLSGSE*

  Nucleotide sequence: 1116 bp    .

>IMU00332 NZ_CP015202:c2923501-2922386 [Pseudomonas putida B6-2]
ATGATCGACCTGAGAAGCCCGGATGCAATCCTGAGCGACTACGCAAAGCGTTACGCCCATTTGCTCCCCGAACTATCGCC
GCAGTTGCAACAGCGCATGGCCTACATGCCGGAGCCCGACGCACCAAGGCTGCCGCTGACAAAGCCCGCCTGGCTGAGCG
ACCGATCCTGCACGTTGACACCAGGCCAGGCGATAGACCGCGCGCAGGGTGCATTGCTGGGCCTGGCGATTGGTGATGCG
GTAGGCACCACCCTGGAGTTCCGGCGCCGCGATCAGGGGCAGGTCAGCGACATGGTCGGCGGTGGGCCGTTCCGCCTCGC
GCCAGGTGAGTGGACCGACGATACCAGCATGGCCCTGTGCCTGGCAGACACCTACGCAAGCCAAGGCAAGTTCGATTTCG
CGACCTTTGCCGATGCCATGGTGCGCTGGTACCGGCAGGGCGAAAACAGCGCCAATGGCCGCTGCTTCGACATCGGCAAT
GTCACCCGCCGAGCCCTGGAAGGGTGGGAGGCCCAAGGGCTTGCGTGGATGGGCAATCTCGAGGCCTCGACAGCGGGCAA
CGGTTCGTTGATACGGCTCGCGCCTACGGCCATTTTCCGACGCCACTCGCTGTCGGCAACCTGGTGGGAGAGTGTGACGC
AGAGCAGCGTGACCCATCGCGCGATTGAGGTCTCGGAGTGCTGCAAGTTGTTCGGTGCACAGCTGCATTTGGCGCTCAAT
GGCGCGGACAAGGAAGAGGCGTTGTCGCCGAAGGTGCGCCCCCTGCAGCCCCGGGCCTTGATCATCAATGCCGGGGAGTA
CAAGCACAAAACCCGTGAGCAGATTCGCTCGTCAGGGTATGTGGTCGATACCTTGGAAGCAGCGCTCTGGGCTGTGTGGA
ATACGGACAATTTCAGGGATGCGATTCTGTTGGCTGCCAACCTTGCCGATGATGCCGACAGCGTGGCGGCGACGGCAGGG
CAGATTGCGGGCGCCTTGTATGGGGTGTCGGGCATGCCGCCGGAATGGGTGGCCAAGGTTGCCTGGTCGCGGCACATTTG
TGAACTGGCAAGCCGGTTGTTTGAACTCGCGCCTCAGGGGGATGAAATGGACGAGCTGCTATCTGGCAGTGAGTGA