Detailed information of immunity protein


Summary

Immunity protein ID IMU00330 experimental
Cognate effector EFF01495 experimental
Related T6SS (Type) -
Strain Listeria monocytogenes EGD-e
Replicon chromosome
Sequence Protein sequence (328 a.a.); Nucleotide sequence (984 bp)
Description Eri1, but not a predicted catalytically inactive variant (Eri1D69N), significantly increased the viability of E. coli experiencing intoxication by Tre1tox-Sp
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) lmo2786 (Eri1)
Coordinates (Strand) 2872932..2873915 (-)
NCBI ID 16804823
RefSeq NC_003210
Uniprot ID Q8Y3P8_LISMO
KEGG ID lmo:lmo2786
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ADP_ribosyl_GH PF03747.16 7.9e-51 7..296
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-327 MKTRVFGVLAGGALGDAMGMPTECWSQEKIRAIYPNGVETLLPSDESDAFGRKLAAGSITDDTINVLMIAEMIKKNNGQIRVEDYVAELVEWNANSGVSEFVSGPSTLRALKQISEGVPITKTGISGTTNGASMKIAPIGIIANYEDMEDLVSKVHQICLPTHNAKIAIQGASAVAAAISYVARGGNIVDKIWELAYEAIEVASNYGYDFPSASLKFRMDKAREIIGEETGPATALKRLYEEIGSGMETIETIPCAFAIVELAKGDPVEAAKLSACIGWDTDTIGAISAGICGGMSPTIPENYIEMIERVNNINLTQVSEDLYIHVL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.7956 pos: 19-20. AMG-MP. MKTRVFGVLAGGALGDAMG


  Protein sequence: 328 a.a.    .

>IMU00330 NC_003210:c2873915-2872932 [Listeria monocytogenes EGD-e]
MKTRVFGVLAGGALGDAMGMPTECWSQEKIRAIYPNGVETLLPSDESDAFGRKLAAGSITDDTINVLMIAEMIKKNNGQI
RVEDYVAELVEWNANSGVSEFVSGPSTLRALKQISEGVPITKTGISGTTNGASMKIAPIGIIANYEDMEDLVSKVHQICL
PTHNAKIAIQGASAVAAAISYVARGGNIVDKIWELAYEAIEVASNYGYDFPSASLKFRMDKAREIIGEETGPATALKRLY
EEIGSGMETIETIPCAFAIVELAKGDPVEAAKLSACIGWDTDTIGAISAGICGGMSPTIPENYIEMIERVNNINLTQVSE
DLYIHVL*

  Nucleotide sequence: 984 bp    .

>IMU00330 NC_003210:c2873915-2872932 [Listeria monocytogenes EGD-e]
ATGAAAACAAGGGTTTTTGGAGTTTTAGCGGGTGGAGCACTCGGCGATGCAATGGGAATGCCGACAGAATGTTGGTCGCA
GGAAAAGATAAGAGCCATTTATCCTAATGGGGTTGAAACGCTCTTGCCTTCCGACGAAAGTGATGCATTTGGAAGAAAAC
TGGCGGCTGGGTCGATTACAGATGATACGATAAATGTGTTGATGATTGCTGAAATGATTAAAAAAAATAATGGGCAAATC
CGCGTGGAAGACTATGTCGCGGAATTAGTCGAATGGAACGCTAATTCAGGTGTTTCGGAGTTCGTCTCAGGTCCTTCCAC
ACTTAGAGCATTGAAACAAATCTCAGAAGGCGTGCCGATCACCAAAACAGGTATTTCTGGCACAACAAATGGAGCATCGA
TGAAAATAGCGCCAATCGGCATAATAGCTAATTATGAAGATATGGAAGACTTGGTTTCCAAAGTGCATCAAATTTGCCTG
CCTACACATAATGCCAAAATTGCTATTCAGGGCGCAAGTGCTGTCGCGGCTGCAATTAGTTATGTGGCTCGAGGTGGGAA
CATAGTGGATAAAATATGGGAATTAGCTTACGAAGCAATTGAGGTTGCTAGTAATTACGGATATGATTTCCCTTCAGCGT
CGTTAAAATTTAGAATGGACAAGGCTCGCGAAATTATTGGAGAGGAAACAGGACCAGCGACTGCACTGAAAAGACTATAT
GAAGAAATCGGCTCAGGAATGGAAACAATTGAAACTATTCCCTGCGCATTCGCTATTGTTGAATTAGCAAAAGGTGACCC
AGTAGAAGCGGCCAAATTGAGTGCATGCATTGGTTGGGATACCGATACAATTGGAGCTATCAGTGCGGGGATTTGCGGTG
GAATGTCCCCCACAATACCGGAAAATTATATCGAAATGATTGAGCGAGTAAATAACATTAATCTCACGCAAGTTAGTGAA
GATTTATATATACATGTTCTTTAA