Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00301  |
| Cognate effector | EFF01475 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (154 a.a.); Nucleotide sequence (462 bp) |
| Description | PFL_6210, confers immunity to the activity of the C-terminal domain of PFL_6209 (PFL_6209tox). |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_6210 (Tni2) |
| Coordinates (Strand) | 7050414..7050875 (+) |
| NCBI ID | 70733493 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054E2F8 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-153 | VISDFERIREDGKVIDENMTVDQMIALGWSPCRVVEARWRWQEQLLSVVNSRGLLAIVVPDRQHLAILWNDDDTGVAATLYVVSGDRQQQIRIADQLLINGQLEAGIYSWFEQFPQVSPSIFTCMFSRQRDQAMFRVDIDASTGDIVSIQHSR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9951 | - | - |
Protein sequence: 154 a.a. .
>IMU00301 NC_004129:7050414-7050875 [Pseudomonas protegens Pf-5]
VISDFERIREDGKVIDENMTVDQMIALGWSPCRVVEARWRWQEQLLSVVNSRGLLAIVVPDRQHLAILWNDDDTGVAATL
YVVSGDRQQQIRIADQLLINGQLEAGIYSWFEQFPQVSPSIFTCMFSRQRDQAMFRVDIDASTGDIVSIQHSR*
VISDFERIREDGKVIDENMTVDQMIALGWSPCRVVEARWRWQEQLLSVVNSRGLLAIVVPDRQHLAILWNDDDTGVAATL
YVVSGDRQQQIRIADQLLINGQLEAGIYSWFEQFPQVSPSIFTCMFSRQRDQAMFRVDIDASTGDIVSIQHSR*
Nucleotide sequence: 462 bp .
>IMU00301 NC_004129:7050414-7050875 [Pseudomonas protegens Pf-5]
GTGATTAGTGATTTCGAGCGGATCCGCGAAGACGGCAAGGTCATCGACGAAAACATGACGGTGGACCAGATGATCGCCCT
GGGGTGGTCTCCTTGCCGGGTCGTCGAGGCTCGCTGGCGCTGGCAGGAACAGCTCCTGAGCGTGGTCAACAGCCGCGGCC
TGCTGGCGATCGTGGTGCCGGATCGGCAACACCTGGCCATCCTGTGGAACGATGACGATACGGGCGTGGCCGCAACCCTC
TACGTGGTCTCCGGCGACCGCCAGCAACAGATCAGGATTGCCGACCAGTTACTGATCAATGGCCAGCTCGAGGCAGGTAT
CTACAGCTGGTTCGAACAGTTCCCGCAGGTTTCGCCCAGCATCTTTACCTGCATGTTCAGCCGTCAGCGTGACCAGGCAA
TGTTCAGGGTGGATATCGACGCGTCCACTGGCGACATTGTTTCGATACAGCATTCGCGCTGA
GTGATTAGTGATTTCGAGCGGATCCGCGAAGACGGCAAGGTCATCGACGAAAACATGACGGTGGACCAGATGATCGCCCT
GGGGTGGTCTCCTTGCCGGGTCGTCGAGGCTCGCTGGCGCTGGCAGGAACAGCTCCTGAGCGTGGTCAACAGCCGCGGCC
TGCTGGCGATCGTGGTGCCGGATCGGCAACACCTGGCCATCCTGTGGAACGATGACGATACGGGCGTGGCCGCAACCCTC
TACGTGGTCTCCGGCGACCGCCAGCAACAGATCAGGATTGCCGACCAGTTACTGATCAATGGCCAGCTCGAGGCAGGTAT
CTACAGCTGGTTCGAACAGTTCCCGCAGGTTTCGCCCAGCATCTTTACCTGCATGTTCAGCCGTCAGCGTGACCAGGCAA
TGTTCAGGGTGGATATCGACGCGTCCACTGGCGACATTGTTTCGATACAGCATTCGCGCTGA