Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00300  |
| Cognate effector | EFF01474 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (209 a.a.); Nucleotide sequence (627 bp) |
| Description | PFL_6097 confers immunity to the C-terminal domain of RhsA (RhsAtox)-mediated toxicity. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_6097 (rhsI) |
| Coordinates (Strand) | 6929889..6930515 (+) |
| NCBI ID | 70733380 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054E288 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-208 | MKADTKELRSNFEKALQLFLKSELSGYPGVKVTKNKVVVAQKESGAVATLHFEYLTNTRAYEILIFVEHPALQAEIDQINPPYPSNLANPKFIYSLSTVSEKAACPLLPVTEQGIENTCQVILRQLQDVHLPILFNLFNVSPALVSDVIDRPKYYSYPVPLIFIALKTNKIKPDDETLAKIMSEQTLGYTGHQDLKESFNKQLLAALN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9918 | - | - |
Protein sequence: 209 a.a. .
>IMU00300 NC_004129:6929889-6930515 [Pseudomonas protegens Pf-5]
MKADTKELRSNFEKALQLFLKSELSGYPGVKVTKNKVVVAQKESGAVATLHFEYLTNTRAYEILIFVEHPALQAEIDQIN
PPYPSNLANPKFIYSLSTVSEKAACPLLPVTEQGIENTCQVILRQLQDVHLPILFNLFNVSPALVSDVIDRPKYYSYPVP
LIFIALKTNKIKPDDETLAKIMSEQTLGYTGHQDLKESFNKQLLAALN*
MKADTKELRSNFEKALQLFLKSELSGYPGVKVTKNKVVVAQKESGAVATLHFEYLTNTRAYEILIFVEHPALQAEIDQIN
PPYPSNLANPKFIYSLSTVSEKAACPLLPVTEQGIENTCQVILRQLQDVHLPILFNLFNVSPALVSDVIDRPKYYSYPVP
LIFIALKTNKIKPDDETLAKIMSEQTLGYTGHQDLKESFNKQLLAALN*
Nucleotide sequence: 627 bp .
>IMU00300 NC_004129:6929889-6930515 [Pseudomonas protegens Pf-5]
ATGAAAGCAGACACCAAGGAATTGCGCAGCAACTTTGAAAAGGCACTCCAGCTTTTTTTGAAGAGCGAGCTCTCGGGTTA
CCCCGGAGTAAAAGTCACCAAGAACAAAGTGGTGGTTGCACAGAAAGAATCGGGCGCCGTGGCGACCCTGCATTTCGAAT
ACCTGACCAACACCCGCGCCTACGAAATCCTCATCTTCGTCGAACACCCGGCCCTGCAGGCCGAGATCGACCAGATAAAC
CCGCCCTACCCGTCCAACCTGGCCAATCCGAAATTTATCTACAGCTTGTCGACGGTCAGTGAAAAAGCTGCCTGCCCGCT
ACTGCCCGTGACAGAGCAAGGTATTGAAAATACCTGCCAGGTGATTCTGCGCCAGCTCCAGGATGTTCACCTGCCTATTC
TGTTCAACCTCTTCAACGTCAGCCCCGCGCTGGTGAGCGATGTCATAGACAGGCCAAAGTATTACTCCTATCCGGTCCCA
CTGATTTTTATCGCCTTGAAAACCAACAAAATCAAACCGGACGACGAAACGCTAGCGAAGATCATGAGCGAGCAGACCCT
GGGCTACACCGGTCATCAGGACCTGAAAGAGTCGTTCAACAAGCAACTACTGGCAGCACTGAACTGA
ATGAAAGCAGACACCAAGGAATTGCGCAGCAACTTTGAAAAGGCACTCCAGCTTTTTTTGAAGAGCGAGCTCTCGGGTTA
CCCCGGAGTAAAAGTCACCAAGAACAAAGTGGTGGTTGCACAGAAAGAATCGGGCGCCGTGGCGACCCTGCATTTCGAAT
ACCTGACCAACACCCGCGCCTACGAAATCCTCATCTTCGTCGAACACCCGGCCCTGCAGGCCGAGATCGACCAGATAAAC
CCGCCCTACCCGTCCAACCTGGCCAATCCGAAATTTATCTACAGCTTGTCGACGGTCAGTGAAAAAGCTGCCTGCCCGCT
ACTGCCCGTGACAGAGCAAGGTATTGAAAATACCTGCCAGGTGATTCTGCGCCAGCTCCAGGATGTTCACCTGCCTATTC
TGTTCAACCTCTTCAACGTCAGCCCCGCGCTGGTGAGCGATGTCATAGACAGGCCAAAGTATTACTCCTATCCGGTCCCA
CTGATTTTTATCGCCTTGAAAACCAACAAAATCAAACCGGACGACGAAACGCTAGCGAAGATCATGAGCGAGCAGACCCT
GGGCTACACCGGTCATCAGGACCTGAAAGAGTCGTTCAACAAGCAACTACTGGCAGCACTGAACTGA