Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00074  |
| Cognate effector | EFF00434 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (157 a.a.); Nucleotide sequence (471 bp) |
| Description | Tgi2 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_3036 (Tgi2) |
| Coordinates (Strand) | 3502296..3502766 (-) |
| NCBI ID | 70730401 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054D6E3 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-156 | VLKLKSPSWVLGLSLACTTLVACATDSLQPARIKDSGLTREQAEQVLRVALKHQDYQLQRPGVFIDGDLQDENGKPPHPGYYDFSLGYNDPKAGATEYWGLFSVSLNTGDTWEINSCKRLDGAELRALQRRVMARTGKSLADEKSQREGLGCEDQQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9806 | pos: 22-23. LVA-CA. | VLKLKSPSWVLGLSLACTTLVA |
Protein sequence: 157 a.a. .
>IMU00074 NC_004129:c3502766-3502296 [Pseudomonas protegens Pf-5]
VLKLKSPSWVLGLSLACTTLVACATDSLQPARIKDSGLTREQAEQVLRVALKHQDYQLQRPGVFIDGDLQDENGKPPHPG
YYDFSLGYNDPKAGATEYWGLFSVSLNTGDTWEINSCKRLDGAELRALQRRVMARTGKSLADEKSQREGLGCEDQQ*
VLKLKSPSWVLGLSLACTTLVACATDSLQPARIKDSGLTREQAEQVLRVALKHQDYQLQRPGVFIDGDLQDENGKPPHPG
YYDFSLGYNDPKAGATEYWGLFSVSLNTGDTWEINSCKRLDGAELRALQRRVMARTGKSLADEKSQREGLGCEDQQ*
Nucleotide sequence: 471 bp .
>IMU00074 NC_004129:c3502766-3502296 [Pseudomonas protegens Pf-5]
GTGCTGAAACTCAAGAGTCCTTCCTGGGTGCTTGGCCTGTCCCTGGCCTGCACCACACTGGTGGCCTGCGCCACCGATAG
CCTGCAGCCGGCGCGGATCAAGGACAGCGGCCTGACCCGCGAACAGGCGGAGCAGGTCCTGCGGGTGGCCCTCAAGCACC
AGGACTATCAGTTGCAGCGCCCCGGGGTGTTCATCGACGGCGACCTGCAGGACGAAAACGGCAAGCCGCCCCATCCCGGG
TACTACGACTTCAGCCTCGGCTACAACGATCCCAAGGCCGGGGCCACCGAGTACTGGGGCCTGTTCTCGGTCAGCCTGAA
CACGGGCGATACCTGGGAAATCAACAGCTGCAAGCGCCTTGATGGCGCCGAGCTGCGAGCCTTGCAGCGGCGGGTGATGG
CCCGCACCGGCAAGAGCCTGGCGGATGAAAAAAGCCAGCGCGAGGGCCTGGGCTGCGAAGACCAACAATAA
GTGCTGAAACTCAAGAGTCCTTCCTGGGTGCTTGGCCTGTCCCTGGCCTGCACCACACTGGTGGCCTGCGCCACCGATAG
CCTGCAGCCGGCGCGGATCAAGGACAGCGGCCTGACCCGCGAACAGGCGGAGCAGGTCCTGCGGGTGGCCCTCAAGCACC
AGGACTATCAGTTGCAGCGCCCCGGGGTGTTCATCGACGGCGACCTGCAGGACGAAAACGGCAAGCCGCCCCATCCCGGG
TACTACGACTTCAGCCTCGGCTACAACGATCCCAAGGCCGGGGCCACCGAGTACTGGGGCCTGTTCTCGGTCAGCCTGAA
CACGGGCGATACCTGGGAAATCAACAGCTGCAAGCGCCTTGATGGCGCCGAGCTGCGAGCCTTGCAGCGGCGGGTGATGG
CCCGCACCGGCAAGAGCCTGGCGGATGAAAAAAGCCAGCGCGAGGGCCTGGGCTGCGAAGACCAACAATAA